Gene description for Gja1
Gene name gap junction protein, alpha 1
Gene symbol Gja1
Other names/aliases AU042049
AW546267
Cnx43
Cx43
Cx43alpha1
Gja-1
Npm1
connexin43
Species Mus musculus
 Database cross references - Gja1
ExoCarta ExoCarta_14609
Vesiclepedia VP_14609
Entrez Gene 14609
UniProt P23242  
 Gja1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Gja1
Molecular Function
    signaling receptor binding GO:0005102 IPI
    gap junction channel activity GO:0005243 IBA
    gap junction channel activity GO:0005243 IDA
    gap junction channel activity GO:0005243 IMP
    gap junction channel activity GO:0005243 ISO
    protein binding GO:0005515 IPI
    beta-catenin binding GO:0008013 IEA
    beta-catenin binding GO:0008013 ISO
    monoatomic ion transmembrane transporter activity GO:0015075 IEA
    monoatomic ion transmembrane transporter activity GO:0015075 ISO
    efflux transmembrane transporter activity GO:0015562 IMP
    tubulin binding GO:0015631 ISO
    tubulin binding GO:0015631 ISS
    SH3 domain binding GO:0017124 IEA
    SH3 domain binding GO:0017124 ISO
    protein domain specific binding GO:0019904 ISO
    PDZ domain binding GO:0030165 IEA
    PDZ domain binding GO:0030165 ISO
    glutathione transmembrane transporter activity GO:0034634 IMP
    alpha-tubulin binding GO:0043014 ISO
    beta-tubulin binding GO:0048487 IDA
    gap junction hemi-channel activity GO:0055077 ISO
    gap junction hemi-channel activity GO:0055077 ISS
    connexin binding GO:0071253 IEA
    connexin binding GO:0071253 ISO
    gap junction channel activity involved in cardiac conduction electrical coupling GO:0086075 TAS
    scaffold protein binding GO:0097110 IPI
    disordered domain specific binding GO:0097718 IEA
    disordered domain specific binding GO:0097718 ISO
    gap junction channel activity involved in cell communication by electrical coupling GO:1903763 ISO
    protein tyrosine kinase binding GO:1990782 IPI
Biological Process
    establishment of mitotic spindle orientation GO:0000132 IEA
    establishment of mitotic spindle orientation GO:0000132 ISO
    osteoblast differentiation GO:0001649 IMP
    in utero embryonic development GO:0001701 IMP
    neuron migration GO:0001764 IMP
    negative regulation of endothelial cell proliferation GO:0001937 IEA
    negative regulation of endothelial cell proliferation GO:0001937 ISO
    heart looping GO:0001947 IMP
    columnar/cuboidal epithelial cell maturation GO:0002069 IMP
    lens development in camera-type eye GO:0002088 IMP
    chronic inflammatory response GO:0002544 IEA
    chronic inflammatory response GO:0002544 ISO
    response to ischemia GO:0002931 IEA
    positive regulation of glomerular filtration GO:0003104 IEA
    positive regulation of glomerular filtration GO:0003104 ISO
    endothelium development GO:0003158 IEA
    atrial ventricular junction remodeling GO:0003294 IGI
    epicardial cell to mesenchymal cell transition GO:0003347 TAS
    signal transduction GO:0007165 IEA
    signal transduction GO:0007165 ISO
    positive regulation of cytosolic calcium ion concentration GO:0007204 IEA
    positive regulation of cytosolic calcium ion concentration GO:0007204 ISO
    cell-cell signaling GO:0007267 IBA
    cell-cell signaling GO:0007267 IDA
    cell-cell signaling GO:0007267 ISO
    spermatogenesis GO:0007283 IMP
    spermatogenesis GO:0007283 ISO
    heart development GO:0007507 IBA
    heart development GO:0007507 IMP
    heart development GO:0007507 IMP
    adult heart development GO:0007512 IMP
    regulation of heart contraction GO:0008016 IMP
    protein localization GO:0008104 ISO
    negative regulation of cell population proliferation GO:0008285 ISO
    male gonad development GO:0008584 IMP
    response to pH GO:0009268 ISO
    response to glucose GO:0009749 IEA
    vascular transport GO:0010232 IEA
    vascular transport GO:0010232 ISO
    positive regulation of gene expression GO:0010628 IMP
    positive regulation of gene expression GO:0010628 ISO
    negative regulation of gene expression GO:0010629 IMP
    cell communication by chemical coupling GO:0010643 IDA
    cell communication by electrical coupling GO:0010644 IBA
    cell communication by electrical coupling GO:0010644 IDA
    cell communication by electrical coupling GO:0010644 ISO
    regulation of cell communication by electrical coupling GO:0010649 IEA
    regulation of cell communication by electrical coupling GO:0010649 ISO
    positive regulation of cell communication by chemical coupling GO:0010652 IEA
    positive regulation of cell communication by chemical coupling GO:0010652 ISO
    glutamate secretion GO:0014047 IMP
    ATP transport GO:0015867 IEA
    ATP transport GO:0015867 ISO
    negative regulation of cell growth GO:0030308 IMP
    negative regulation of cell growth GO:0030308 ISO
    regulation of bone mineralization GO:0030500 IMP
    positive regulation of insulin secretion GO:0032024 IEA
    positive regulation of insulin secretion GO:0032024 ISO
    negative regulation of gonadotropin secretion GO:0032277 IEA
    negative regulation of gonadotropin secretion GO:0032277 ISO
    response to estradiol GO:0032355 IEA
    response to lipopolysaccharide GO:0032496 IEA
    response to retinoic acid GO:0032526 IEA
    monoatomic ion transmembrane transport GO:0034220 IEA
    monoatomic ion transmembrane transport GO:0034220 ISO
    response to fluid shear stress GO:0034405 IEA
    glutathione transmembrane transport GO:0034775 IEA
    embryonic heart tube development GO:0035050 IMP
    maintenance of protein localization in endoplasmic reticulum GO:0035437 IEA
    maintenance of protein localization in endoplasmic reticulum GO:0035437 ISO
    T cell proliferation GO:0042098 IMP
    T cell activation GO:0042110 IDA
    vasodilation GO:0042311 IEA
    vasodilation GO:0042311 ISO
    embryonic digit morphogenesis GO:0042733 IMP
    xenobiotic transport GO:0042908 IMP
    regulation of apoptotic process GO:0042981 ISO
    regulation of apoptotic process GO:0042981 ISS
    skeletal muscle tissue regeneration GO:0043403 IDA
    response to peptide hormone GO:0043434 IEA
    cell-cell junction organization GO:0045216 IMP
    positive regulation of protein catabolic process GO:0045732 IEA
    positive regulation of protein catabolic process GO:0045732 ISO
    positive regulation of meiotic nuclear division GO:0045836 IEA
    positive regulation of meiotic nuclear division GO:0045836 ISO
    positive regulation of striated muscle tissue development GO:0045844 IMP
    positive regulation of vasoconstriction GO:0045907 IEA
    positive regulation of vasoconstriction GO:0045907 ISO
    decidualization GO:0046697 IEA
    bone remodeling GO:0046849 IMP
    regulation of bone remodeling GO:0046850 IMP
    blood vessel morphogenesis GO:0048514 IMP
    neuron projection morphogenesis GO:0048812 IEA
    neuron projection morphogenesis GO:0048812 ISO
    regulation of calcium ion transport GO:0051924 IEA
    regulation of calcium ion transport GO:0051924 ISO
    transmembrane transport GO:0055085 ISO
    negative regulation of cardiac muscle cell proliferation GO:0060044 IEA
    negative regulation of cardiac muscle cell proliferation GO:0060044 ISO
    milk ejection reflex GO:0060156 IMP
    regulation of ventricular cardiac muscle cell membrane repolarization GO:0060307 IGI
    regulation of ventricular cardiac muscle cell membrane repolarization GO:0060307 IMP
    regulation of blood vessel remodeling GO:0060312 TAS
    bone development GO:0060348 IMP
    regulation of atrial cardiac muscle cell membrane depolarization GO:0060371 IMP
    regulation of ventricular cardiac muscle cell membrane depolarization GO:0060373 IGI
    regulation of ventricular cardiac muscle cell membrane depolarization GO:0060373 IMP
    cardiac conduction GO:0061337 IMP
    cellular response to mechanical stimulus GO:0071260 IEA
    cellular response to parathyroid hormone stimulus GO:0071374 IEA
    cellular response to pH GO:0071467 IDA
    cell communication by electrical coupling involved in cardiac conduction GO:0086064 IEA
    establishment of epithelial cell polarity GO:0090162 TAS
    neuroblast migration GO:0097402 IMP
    microtubule-based transport GO:0099111 ISO
    microtubule-based transport GO:0099111 ISS
    regulation of actin filament organization GO:0110053 IEA
    regulation of actin filament organization GO:0110053 ISO
    positive regulation of cold-induced thermogenesis GO:0120162 IMP
    export across plasma membrane GO:0140115 IMP
    negative regulation of trophoblast cell migration GO:1901164 IEA
    negative regulation of trophoblast cell migration GO:1901164 ISO
    positive regulation of establishment of Sertoli cell barrier GO:1904446 IEA
    positive regulation of establishment of Sertoli cell barrier GO:1904446 ISO
    cellular response to amyloid-beta GO:1904646 IMP
    positive regulation of vascular associated smooth muscle cell proliferation GO:1904707 IEA
    positive regulation of vascular associated smooth muscle cell proliferation GO:1904707 ISO
    positive regulation of morphogenesis of an epithelium GO:1905332 IEA
    positive regulation of morphogenesis of an epithelium GO:1905332 ISO
    positive regulation of mesodermal cell differentiation GO:1905772 IEA
    positive regulation of mesodermal cell differentiation GO:1905772 ISO
    epididymis development GO:1905867 IEA
    negative regulation of DNA biosynthetic process GO:2000279 IEA
    negative regulation of DNA biosynthetic process GO:2000279 ISO
    positive regulation of stem cell proliferation GO:2000648 IEA
    positive regulation of stem cell proliferation GO:2000648 ISO
    regulation of bicellular tight junction assembly GO:2000810 IEA
    regulation of bicellular tight junction assembly GO:2000810 ISO
    positive regulation of behavioral fear response GO:2000987 IEA
    positive regulation of behavioral fear response GO:2000987 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 ISO
    mitochondrion GO:0005739 ISS
    mitochondrial outer membrane GO:0005741 IEA
    mitochondrial outer membrane GO:0005741 ISO
    endosome GO:0005768 ISO
    early endosome GO:0005769 IEA
    early endosome GO:0005769 ISO
    late endosome GO:0005770 ISO
    multivesicular body GO:0005771 IEA
    multivesicular body GO:0005771 ISO
    endoplasmic reticulum GO:0005783 IEA
    Golgi apparatus GO:0005794 IDA
    Golgi apparatus GO:0005794 ISO
    cytosol GO:0005829 IDA
    intermediate filament GO:0005882 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    cell-cell junction GO:0005911 IDA
    fascia adherens GO:0005916 IDA
    fascia adherens GO:0005916 ISO
    gap junction GO:0005921 IDA
    gap junction GO:0005921 ISO
    connexin complex GO:0005922 IBA
    connexin complex GO:0005922 ISO
    connexin complex GO:0005922 ISS
    intercalated disc GO:0014704 IDA
    intercalated disc GO:0014704 ISO
    membrane GO:0016020 IDA
    membrane GO:0016020 ISO
    apical plasma membrane GO:0016324 IDA
    lateral plasma membrane GO:0016328 IDA
    cell junction GO:0030054 IDA
    cell junction GO:0030054 ISO
    protein-containing complex GO:0032991 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    contractile muscle fiber GO:0043292 IDA
    cell-cell contact zone GO:0044291 ISO
    membrane raft GO:0045121 IEA
    membrane raft GO:0045121 ISO
    tight junction GO:0070160 IEA
    tight junction GO:0070160 ISO
 Experiment description of studies that identified Gja1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Gja1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Usp2  
Biochemical Activity Rattus norvegicus
2 Nfkbia  
Affinity Capture-Western Rattus norvegicus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Prkca 18750
Co-fractionation Mus musculus
5 Was  
Affinity Capture-Western Rattus norvegicus
6 Cdh2 12558
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
7 Jup 16480
Affinity Capture-Western Mus musculus
8 Eed  
Affinity Capture-MS Mus musculus
9 Map1lc3a  
Affinity Capture-Western Mus musculus
10 Prkcb  
Co-fractionation Mus musculus
11 Kalrn  
Affinity Capture-MS Mus musculus
12 Sqstm1  
Affinity Capture-Western Mus musculus
13 Tsg101 22088
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
14 Ctnnb1 12387
Affinity Capture-Western Mus musculus
15 Nbr1  
Affinity Capture-Western Mus musculus
16 Tjp1  
Co-fractionation Mus musculus
17 Eps15 13858
Affinity Capture-Western Mus musculus
18 Usp48  
Biochemical Activity Rattus norvegicus
19 Prkce  
Co-fractionation Mus musculus
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