Gene description for Ap2a2
Gene name adaptor-related protein complex 2, alpha 2 subunit
Gene symbol Ap2a2
Other names/aliases 2410074K14Rik
AF006990
AW146353
Adtab
C78001
L25
Species Mus musculus
 Database cross references - Ap2a2
ExoCarta ExoCarta_11772
Vesiclepedia VP_11772
Entrez Gene 11772
UniProt P17427  
 Ap2a2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ap2a2
Molecular Function
    protein binding GO:0005515 IPI
    lipid binding GO:0008289 IEA
    kinase binding GO:0019900 IPI
    protein kinase binding GO:0019901 IPI
    protein domain specific binding GO:0019904 IPI
    phosphatidylinositol binding GO:0035091 ISO
    clathrin adaptor activity GO:0035615 IBA
    clathrin adaptor activity GO:0035615 IEA
    protein-containing complex binding GO:0044877 ISO
    disordered domain specific binding GO:0097718 IPI
    protein serine/threonine kinase binding GO:0120283 ISO
Biological Process
    intracellular protein transport GO:0006886 TAS
    vesicle-mediated transport GO:0016192 NAS
    vesicle-mediated transport GO:0016192 TAS
    synaptic vesicle endocytosis GO:0048488 IDA
    synaptic vesicle endocytosis GO:0048488 IMP
    synaptic vesicle endocytosis GO:0048488 NAS
    clathrin-dependent endocytosis GO:0072583 IBA
    clathrin-dependent endocytosis GO:0072583 IDA
    clathrin-dependent endocytosis GO:0072583 ISO
    clathrin-dependent endocytosis GO:0072583 NAS
    postsynaptic neurotransmitter receptor internalization GO:0098884 NAS
    regulation of hematopoietic stem cell differentiation GO:1902036 NAS
Subcellular Localization
    synaptic vesicle GO:0008021 ISO
    cytoplasmic side of plasma membrane GO:0009898 NAS
    membrane coat GO:0030117 IDA
    AP-2 adaptor complex GO:0030122 IBA
    AP-2 adaptor complex GO:0030122 IDA
    AP-2 adaptor complex GO:0030122 ISO
    AP-2 adaptor complex GO:0030122 NAS
    secretory granule GO:0030141 TAS
    cytoplasmic vesicle GO:0031410 IDA
    presynapse GO:0098793 IEA
    postsynapse GO:0098794 IEA
 Experiment description of studies that identified Ap2a2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ap2a2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Epn1  
Reconstituted Complex Rattus norvegicus
2 Fxr1 14359
Affinity Capture-MS Mus musculus
3 Ubc  
Reconstituted Complex Mus musculus
4 Eps15 313474
Reconstituted Complex Rattus norvegicus
5 Bin1  
Reconstituted Complex Rattus norvegicus
6 Ap2b1 71770
Co-fractionation Mus musculus
7 Tnik  
Affinity Capture-MS Mus musculus
8 Numb  
Co-fractionation Mus musculus
9 Atxn1  
Affinity Capture-MS Mus musculus
10 Kctd13  
Affinity Capture-MS Mus musculus
11 Amph  
Reconstituted Complex Rattus norvegicus
12 Snap91  
Co-fractionation Mus musculus
13 Pex5l  
Affinity Capture-MS Mus musculus
14 Epn1  
Co-fractionation Mus musculus
15 Snap91  
Reconstituted Complex Rattus norvegicus
16 Dnm1 140694
Reconstituted Complex Rattus norvegicus
17 Fzr1  
Affinity Capture-MS Mus musculus
18 Atg5  
Affinity Capture-MS Mus musculus
19 Eps15 13858
Co-fractionation Mus musculus
20 Sptbn2  
Two-hybrid Rattus norvegicus
21 Aagab  
Two-hybrid Rattus norvegicus
22 TXNIP 10628
Affinity Capture-Western Homo sapiens
23 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
24 Eed  
Affinity Capture-MS Mus musculus
25 Agap2  
Affinity Capture-MS Mus musculus
26 Tia1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Ap2a2 is involved
PathwayEvidenceSource
Adaptive Immune System IEA Reactome
Axon guidance IEA Reactome
Beta-catenin independent WNT signaling IEA Reactome
Cargo recognition for clathrin-mediated endocytosis IEA Reactome
Clathrin-mediated endocytosis IEA Reactome
Developmental Biology IEA Reactome
Glutamate binding, activation of AMPA receptors and synaptic plasticity IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
L1CAM interactions IEA Reactome
LDL clearance IEA Reactome
Membrane Trafficking IEA Reactome
MHC class II antigen presentation IEA Reactome
Nervous system development IEA Reactome
Neuronal System IEA Reactome
Neurotransmitter receptors and postsynaptic signal transmission IEA Reactome
Neutrophil degranulation IEA Reactome
PCP/CE pathway IEA Reactome
Plasma lipoprotein assembly, remodeling, and clearance IEA Reactome
Plasma lipoprotein clearance IEA Reactome
Recycling pathway of L1 IEA Reactome
Retrograde neurotrophin signalling IEA Reactome
Signal Transduction IEA Reactome
Signaling by NTRK1 (TRKA) IEA Reactome
Signaling by NTRKs IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by WNT IEA Reactome
Trafficking of AMPA receptors IEA Reactome
Trafficking of GluR2-containing AMPA receptors IEA Reactome
Transmission across Chemical Synapses IEA Reactome
Transport of small molecules IEA Reactome
Vesicle-mediated transport IEA Reactome
VLDLR internalisation and degradation IEA Reactome
WNT5A-dependent internalization of FZD2, FZD5 and ROR2 IEA Reactome
WNT5A-dependent internalization of FZD4 IEA Reactome





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