Gene description for Ap2a1
Gene name adaptor-related protein complex 2, alpha 1 subunit
Gene symbol Ap2a1
Other names/aliases Adtaa
Species Mus musculus
 Database cross references - Ap2a1
ExoCarta ExoCarta_11771
Vesiclepedia VP_11771
Entrez Gene 11771
UniProt P17426  
 Ap2a1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ap2a1
Molecular Function
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IPI
    clathrin adaptor activity GO:0035615 IBA
    clathrin adaptor activity GO:0035615 IEA
    protein-containing complex binding GO:0044877 IEA
    protein-containing complex binding GO:0044877 ISO
    low-density lipoprotein particle receptor binding GO:0050750 IEA
    low-density lipoprotein particle receptor binding GO:0050750 ISO
Biological Process
    intracellular protein transport GO:0006886 TAS
    positive regulation of neuron projection development GO:0010976 IEA
    positive regulation of neuron projection development GO:0010976 ISO
    vesicle-mediated transport GO:0016192 NAS
    vesicle-mediated transport GO:0016192 TAS
    positive regulation of receptor-mediated endocytosis GO:0048260 IEA
    positive regulation of receptor-mediated endocytosis GO:0048260 ISO
    synaptic vesicle endocytosis GO:0048488 IDA
    synaptic vesicle endocytosis GO:0048488 IMP
    synaptic vesicle endocytosis GO:0048488 NAS
    clathrin-dependent endocytosis GO:0072583 IBA
    clathrin-dependent endocytosis GO:0072583 IDA
    clathrin-dependent endocytosis GO:0072583 ISO
    clathrin-dependent endocytosis GO:0072583 NAS
    postsynaptic neurotransmitter receptor internalization GO:0098884 NAS
    negative regulation of hyaluronan biosynthetic process GO:1900126 ISO
    negative regulation of hyaluronan biosynthetic process GO:1900126 ISS
Subcellular Localization
    synaptic vesicle GO:0008021 IEA
    cytoplasmic side of plasma membrane GO:0009898 NAS
    basolateral plasma membrane GO:0016323 IEA
    basolateral plasma membrane GO:0016323 ISO
    apical plasma membrane GO:0016324 IEA
    apical plasma membrane GO:0016324 ISO
    membrane coat GO:0030117 IDA
    AP-2 adaptor complex GO:0030122 IBA
    AP-2 adaptor complex GO:0030122 IDA
    AP-2 adaptor complex GO:0030122 ISO
    AP-2 adaptor complex GO:0030122 NAS
    clathrin-coated vesicle GO:0030136 ISO
    secretory granule GO:0030141 TAS
    filopodium tip GO:0032433 IDA
    protein-containing complex GO:0032991 ISO
    postsynaptic endocytic zone GO:0098843 IDA
    glutamatergic synapse GO:0098978 IDA
 Experiment description of studies that identified Ap2a1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ap2a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Pex5l  
Affinity Capture-MS Mus musculus
2 Nckap1 50884
Affinity Capture-MS Mus musculus
3 Cyfip2 76884
Affinity Capture-MS Mus musculus
4 Fxr1 14359
Affinity Capture-MS Mus musculus
5 Ubc  
Reconstituted Complex Mus musculus
6 SNCA 6622
Affinity Capture-MS Homo sapiens
7 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
8 Foxp3  
Affinity Capture-MS Mus musculus
9 Eed  
Affinity Capture-MS Mus musculus
10 Atg5  
Affinity Capture-MS Mus musculus
11 Eps15 13858
Co-fractionation Mus musculus
12 Mapt  
Affinity Capture-MS Mus musculus
13 Agap2  
Affinity Capture-MS Mus musculus
14 Snap25 20614
Affinity Capture-MS Mus musculus
15 Syt7  
Co-fractionation Mus musculus
16 Snap29  
Co-fractionation Mus musculus
17 Synj1 104015
Co-fractionation Mus musculus
18 Tia1  
Affinity Capture-MS Mus musculus
19 Cyfip1 20430
Affinity Capture-MS Mus musculus
20 Kctd13  
Affinity Capture-MS Mus musculus
21 Tnik  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Ap2a1 is involved
PathwayEvidenceSource
Adaptive Immune System IEA Reactome
Axon guidance IEA Reactome
Beta-catenin independent WNT signaling IEA Reactome
Cargo recognition for clathrin-mediated endocytosis IEA Reactome
Clathrin-mediated endocytosis IEA Reactome
Developmental Biology IEA Reactome
Glutamate binding, activation of AMPA receptors and synaptic plasticity IEA Reactome
Immune System IEA Reactome
L1CAM interactions IEA Reactome
LDL clearance IEA Reactome
Membrane Trafficking IEA Reactome
MHC class II antigen presentation IEA Reactome
Nervous system development IEA Reactome
Neuronal System IEA Reactome
Neurotransmitter receptors and postsynaptic signal transmission IEA Reactome
PCP/CE pathway IEA Reactome
Plasma lipoprotein assembly, remodeling, and clearance IEA Reactome
Plasma lipoprotein clearance IEA Reactome
Recycling pathway of L1 IEA Reactome
Retrograde neurotrophin signalling IEA Reactome
Signal Transduction IEA Reactome
Signaling by NTRK1 (TRKA) IEA Reactome
Signaling by NTRKs IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by WNT IEA Reactome
Trafficking of AMPA receptors IEA Reactome
Trafficking of GluR2-containing AMPA receptors IEA Reactome
Transmission across Chemical Synapses IEA Reactome
Transport of small molecules IEA Reactome
Vesicle-mediated transport IEA Reactome
VLDLR internalisation and degradation IEA Reactome
WNT5A-dependent internalization of FZD2, FZD5 and ROR2 IEA Reactome
WNT5A-dependent internalization of FZD4 IEA Reactome





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