Gene description for ARHGAP26
Gene name Rho GTPase activating protein 26
Gene symbol ARHGAP26
Other names/aliases GRAF
GRAF1
OPHN1L
OPHN1L1
Species Homo sapiens
 Database cross references - ARHGAP26
ExoCarta ExoCarta_23092
Vesiclepedia VP_23092
Entrez Gene 23092
HGNC 17073
MIM 605370
UniProt Q9UNA1  
 ARHGAP26 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Thymus 23844026    
 Gene ontology annotations for ARHGAP26
Molecular Function
    GTPase activator activity GO:0005096 IBA
    GTPase activator activity GO:0005096 NAS
    GTPase activator activity GO:0005096 TAS
    protein binding GO:0005515 IPI
    phospholipid binding GO:0005543 IDA
    protein-macromolecule adaptor activity GO:0030674 IDA
Biological Process
    mitophagy GO:0000423 IDA
    signal transduction GO:0007165 IEA
    actin cytoskeleton organization GO:0030036 NAS
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
Subcellular Localization
    mitochondrion GO:0005739 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IEA
    focal adhesion GO:0005925 IEA
    endosome membrane GO:0010008 IDA
 Experiment description of studies that identified ARHGAP26 in exosomes
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ARHGAP26
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CBS 875
Affinity Capture-MS Homo sapiens
2 THOC7 80145
Affinity Capture-MS Homo sapiens
3 ANKS1B 56899
Affinity Capture-MS Homo sapiens
4 MYO16 23026
Affinity Capture-Western Homo sapiens
5 MRPL14 64928
Affinity Capture-MS Homo sapiens
6 PKN3 29941
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
7 CSNK1D 1453
Affinity Capture-MS Homo sapiens
8 COLEC12 81035
Affinity Capture-MS Homo sapiens
9 CTR9 9646
Affinity Capture-MS Homo sapiens
10 TULP1  
Affinity Capture-MS Homo sapiens
11 NYAP2  
Affinity Capture-Western Homo sapiens
12 ARHGAP10 79658
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 RBM25 58517
Affinity Capture-MS Homo sapiens
14 SMURF1 57154
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
15 CDC42EP2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
16 NR3C1 2908
Proximity Label-MS Homo sapiens
17 ENPP7 339221
Affinity Capture-MS Homo sapiens
18 LSM4 25804
Affinity Capture-MS Homo sapiens
19 JPH1 56704
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 EMG1 10436
Affinity Capture-MS Homo sapiens
21 ARHGAP42  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 NUDT21 11051
Affinity Capture-MS Homo sapiens
23 MIB1 57534
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
24 PTEN 5728
Affinity Capture-MS Homo sapiens
25 ACADS 35
Co-fractionation Homo sapiens
26 NHLRC2 374354
Affinity Capture-MS Homo sapiens
27 C1orf35  
Affinity Capture-MS Homo sapiens
28 PYCR1 5831
Affinity Capture-MS Homo sapiens
29 YY1 7528
Affinity Capture-MS Homo sapiens
30 PTK2 5747
Reconstituted Complex Homo sapiens
31 THOC6 79228
Affinity Capture-MS Homo sapiens
32 PLG 5340
Affinity Capture-MS Homo sapiens
33 SOX2  
Affinity Capture-MS Homo sapiens
34 CDC42 998
Biochemical Activity Homo sapiens
35 TCEB3 6924
Affinity Capture-MS Homo sapiens
36 NRXN1  
Affinity Capture-MS Homo sapiens
37 TACO1  
Affinity Capture-MS Homo sapiens
38 PSPC1 55269
Affinity Capture-MS Homo sapiens
39 REPS1 85021
Affinity Capture-MS Homo sapiens
40 CEMIP 57214
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
41 ACTL6A 86
Affinity Capture-MS Homo sapiens
42 CPSF4  
Affinity Capture-MS Homo sapiens
43 TOX4  
Affinity Capture-MS Homo sapiens
44 FIP1L1 81608
Affinity Capture-MS Homo sapiens
45 MAPRE2 10982
Affinity Capture-MS Homo sapiens
46 SF3B6 51639
Affinity Capture-MS Homo sapiens
47 NYAP1  
Affinity Capture-Western Homo sapiens
48 MCTS1 28985
Affinity Capture-MS Homo sapiens
49 QPCTL 54814
Affinity Capture-MS Homo sapiens
50 CSNK1E 1454
Affinity Capture-MS Homo sapiens
51 MICAL1 64780
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 RAB11FIP5 26056
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 RHOA 387
FRET Homo sapiens
Biochemical Activity Homo sapiens
54 CA2 760
Affinity Capture-MS Homo sapiens
55 C2CD2 25966
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 SRPK1 6732
Biochemical Activity Homo sapiens
57 KIAA1522 57648
Affinity Capture-MS Homo sapiens
58 OPHN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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