Gene description for PLK1
Gene name polo-like kinase 1
Gene symbol PLK1
Other names/aliases PLK
STPK13
Species Homo sapiens
 Database cross references - PLK1
ExoCarta ExoCarta_5347
Vesiclepedia VP_5347
Entrez Gene 5347
HGNC 9077
MIM 602098
UniProt P53350  
 PLK1 identified in sEVs derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for PLK1
Molecular Function
    magnesium ion binding GO:0000287 IMP
    protein kinase activity GO:0004672 IDA
    protein kinase activity GO:0004672 IMP
    protein serine/threonine kinase activity GO:0004674 EXP
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 IMP
    protein serine/threonine kinase activity GO:0004674 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IMP
    microtubule binding GO:0008017 IDA
    anaphase-promoting complex binding GO:0010997 IPI
    protein kinase binding GO:0019901 IPI
    identical protein binding GO:0042802 IPI
    protein serine kinase activity GO:0106310 IDA
Biological Process
    mitotic sister chromatid segregation GO:0000070 IMP
    mitotic sister chromatid segregation GO:0000070 TAS
    G2/M transition of mitotic cell cycle GO:0000086 IDA
    G2/M transition of mitotic cell cycle GO:0000086 TAS
    negative regulation of transcription by RNA polymerase II GO:0000122 IMP
    establishment of mitotic spindle orientation GO:0000132 IDA
    mitotic cell cycle GO:0000278 IDA
    mitotic cell cycle GO:0000278 IMP
    mitotic cytokinesis GO:0000281 IDA
    mitotic cytokinesis GO:0000281 TAS
    microtubule bundle formation GO:0001578 IDA
    double-strand break repair GO:0006302 IDA
    protein phosphorylation GO:0006468 IDA
    protein phosphorylation GO:0006468 IMP
    mitotic spindle organization GO:0007052 IBA
    mitotic spindle organization GO:0007052 TAS
    sister chromatid cohesion GO:0007062 TAS
    mitotic chromosome condensation GO:0007076 TAS
    mitotic nuclear membrane disassembly GO:0007077 TAS
    metaphase/anaphase transition of mitotic cell cycle GO:0007091 TAS
    mitotic spindle assembly checkpoint signaling GO:0007094 IMP
    mitotic G2 DNA damage checkpoint signaling GO:0007095 IDA
    centrosome cycle GO:0007098 IMP
    regulation of mitotic cell cycle GO:0007346 IMP
    positive regulation of peptidyl-threonine phosphorylation GO:0010800 IMP
    female meiosis chromosome segregation GO:0016321 IEA
    protein ubiquitination GO:0016567 IDA
    peptidyl-serine phosphorylation GO:0018105 IDA
    regulation of mitotic metaphase/anaphase transition GO:0030071 IDA
    regulation of mitotic metaphase/anaphase transition GO:0030071 IMP
    protein destabilization GO:0031648 IDA
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 IMP
    regulation of cytokinesis GO:0032465 IDA
    regulation of cytokinesis GO:0032465 IMP
    negative regulation of apoptotic process GO:0043066 IMP
    regulation of protein binding GO:0043393 IMP
    homologous chromosome segregation GO:0045143 IEA
    establishment of protein localization GO:0045184 IMP
    negative regulation of cyclin-dependent protein serine/threonine kinase activity GO:0045736 IMP
    positive regulation of proteolysis GO:0045862 IDA
    Golgi inheritance GO:0048313 TAS
    nuclear membrane disassembly GO:0051081 IMP
    positive regulation of ubiquitin-protein transferase activity GO:0051443 IMP
    regulation of cell cycle GO:0051726 TAS
    synaptonemal complex disassembly GO:0070194 IEA
    protein localization to chromatin GO:0071168 IDA
    protein localization to nuclear envelope GO:0090435 IMP
    double-strand break repair via alternative nonhomologous end joining GO:0097681 IDA
    positive regulation of protein localization to nucleus GO:1900182 TAS
    regulation of mitotic spindle assembly GO:1901673 IDA
    regulation of mitotic cell cycle phase transition GO:1901990 TAS
    positive regulation of ubiquitin protein ligase activity GO:1904668 IDA
    regulation of protein localization to cell cortex GO:1904776 IDA
    regulation of anaphase-promoting complex-dependent catabolic process GO:1905784 TAS
    negative regulation of double-strand break repair via homologous recombination GO:2000042 IDA
Subcellular Localization
    kinetochore GO:0000776 IBA
    kinetochore GO:0000776 IDA
    chromatin GO:0000785 IEA
    synaptonemal complex GO:0000795 IEA
    spindle pole GO:0000922 IBA
    spindle pole GO:0000922 IDA
    outer kinetochore GO:0000940 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    centrosome GO:0005813 IBA
    centrosome GO:0005813 IDA
    centriole GO:0005814 IEA
    spindle GO:0005819 IDA
    cytosol GO:0005829 TAS
    spindle microtubule GO:0005876 IDA
    microtubule cytoskeleton GO:0015630 IDA
    midbody GO:0030496 IDA
    centriolar satellite GO:0034451 IEA
    spindle midzone GO:0051233 IDA
    mitotic spindle pole GO:0097431 IEA
 Experiment description of studies that identified PLK1 in sEVs
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PLK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KLHL9 55958
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 CFAP20 29105
Affinity Capture-MS Homo sapiens
3 PSMA6 5687
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
4 FABP3 2170
Affinity Capture-MS Homo sapiens
5 RIT1 6016
Negative Genetic Homo sapiens
6 MCM7 4176
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
7 CRBN  
Affinity Capture-Western Homo sapiens
8 RAP1GAP 5909
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
9 FAM131B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 SNCB 6620
Biochemical Activity Homo sapiens
11 BIRC6 57448
Affinity Capture-MS Homo sapiens
12 Mdm2  
Biochemical Activity Mus musculus
13 HDAC2 3066
Affinity Capture-MS Homo sapiens
14 RGCC 28984
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
15 GTF3C5 9328
Affinity Capture-MS Homo sapiens
16 BTN1A1 696
Affinity Capture-MS Homo sapiens
17 BUB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
18 TUBB3 10381
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
19 PSMA4 5685
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
20 TMF1 7110
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 FOXJ1  
Affinity Capture-MS Homo sapiens
22 PARD3 56288
Proximity Label-MS Homo sapiens
23 GLB1 2720
Two-hybrid Homo sapiens
24 DDX17 10521
Co-fractionation Homo sapiens
25 GFAP 2670
Proximity Label-MS Homo sapiens
26 BRCA1 672
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
27 STK39 27347
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
28 EMD 2010
Proximity Label-MS Homo sapiens
29 MTMR14 64419
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 BUB1B  
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
31 ODF2 4957
Affinity Capture-MS Homo sapiens
32 USP16  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
33 ZNF71  
Two-hybrid Homo sapiens
34 CHFR  
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
35 CHEK2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
36 CLU 1191
Affinity Capture-MS Homo sapiens
37 KLF4  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
38 PKMYT1  
Co-localization Homo sapiens
39 ANKRD28 23243
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 PRKDC 5591
Affinity Capture-MS Homo sapiens
41 APP 351
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
42 SATB2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 APEX1 328
Affinity Capture-RNA Homo sapiens
44 HSPA4 3308
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
45 NEDD1 121441
Affinity Capture-MS Homo sapiens
46 C9orf78 51759
Affinity Capture-MS Homo sapiens
47 CENPU  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
48 CHD1L 9557
Affinity Capture-MS Homo sapiens
49 FBXW7  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
50 CACNG3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 LRP5L  
Two-hybrid Homo sapiens
52 CTBP1 1487
Affinity Capture-Western Homo sapiens
53 MCM2 4171
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
54 BICD1 636
Affinity Capture-MS Homo sapiens
55 SPAG9 9043
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
56 TRIOBP 11078
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
57 COPS5 10987
Affinity Capture-MS Homo sapiens
58 BORA  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 PRC1 9055
Affinity Capture-MS Homo sapiens
60 Sugt1 67955
Biochemical Activity Mus musculus
61 MST1R 4486
Affinity Capture-MS Homo sapiens
62 PSMB6 5694
Affinity Capture-Western Homo sapiens
63 MPP2 4355
Two-hybrid Homo sapiens
64 CIRBP 1153
Affinity Capture-MS Homo sapiens
65 C11orf52 91894
Proximity Label-MS Homo sapiens
66 MCM6 4175
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
67 EPS15L1 58513
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 C6orf136  
Two-hybrid Homo sapiens
69 RHOB 388
Proximity Label-MS Homo sapiens
70 C2orf44  
Affinity Capture-MS Homo sapiens
71 COIL  
Proximity Label-MS Homo sapiens
72 RGS20 8601
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 CENPQ  
Biochemical Activity Homo sapiens
74 ACTL6B  
Two-hybrid Homo sapiens
75 EED  
Affinity Capture-MS Homo sapiens
76 SLC25A46 91137
Proximity Label-MS Homo sapiens
77 KIF2C 11004
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
78 ARHGAP5 394
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 CCDC115 84317
Two-hybrid Homo sapiens
80 TP53I3  
Co-fractionation Homo sapiens
81 MECP2 4204
Affinity Capture-MS Homo sapiens
82 MAVS 57506
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 RAB35 11021
Proximity Label-MS Homo sapiens
84 RABAC1 10567
Two-hybrid Homo sapiens
85 PSMA1 5682
Affinity Capture-Western Homo sapiens
86 OCLN 100506658
Proximity Label-MS Homo sapiens
87 NEDD4 4734
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
88 GORASP1 64689
Biochemical Activity Homo sapiens
89 PSMB3 5691
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
90 DVL2 1856
Affinity Capture-MS Homo sapiens
91 USP37  
Affinity Capture-Western Homo sapiens
92 Haus4  
Affinity Capture-MS Mus musculus
93 RBM25 58517
Affinity Capture-MS Homo sapiens
94 NINL  
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
95 LTBR 4055
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 MED1 5469
Affinity Capture-MS Homo sapiens
97 LPL 4023
Affinity Capture-MS Homo sapiens
98 AURKA 6790
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
99 TNFSF11  
Two-hybrid Homo sapiens
100 VRK3 51231
Two-hybrid Homo sapiens
101 EIF6 3692
Two-hybrid Homo sapiens
102 FBXO5  
Affinity Capture-Western Homo sapiens
103 DRICH1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 MIB1 57534
Proximity Label-MS Homo sapiens
105 DSG1 1828
Affinity Capture-MS Homo sapiens
106 TP53 7157
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
107 ENTHD2  
Affinity Capture-MS Homo sapiens
108 RNF126  
Two-hybrid Homo sapiens
109 PARK2  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
110 PPP6R2 9701
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
111 CXADR 1525
Proximity Label-MS Homo sapiens
112 RAI14 26064
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 KRBA1  
Two-hybrid Homo sapiens
114 SIMC1  
Two-hybrid Homo sapiens
115 TUBA1A 7846
Affinity Capture-Western Homo sapiens
116 MAPRE1 22919
Proximity Label-MS Homo sapiens
117 PON1 5444
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Biochemical Activity Homo sapiens
118 BAG6 7917
Two-hybrid Homo sapiens
119 TUFM 7284
Affinity Capture-MS Homo sapiens
120 OFD1 8481
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
121 WAC  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
122 BRCA2 675
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
123 TRIM47 91107
Affinity Capture-MS Homo sapiens
124 ITSN1 6453
Two-hybrid Homo sapiens
125 CCNB1 891
Co-localization Homo sapiens
126 NUDC 10726
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
127 SNCA 6622
Biochemical Activity Homo sapiens
128 VCP 7415
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
129 NTRK1 4914
Affinity Capture-MS Homo sapiens
130 DCAF7 10238
Affinity Capture-MS Homo sapiens
131 RRM2 6241
Co-fractionation Homo sapiens
132 DNAJB9 4189
Two-hybrid Homo sapiens
133 PLK1 5347
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
134 DCTN1 1639
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
135 FLRT1  
Affinity Capture-MS Homo sapiens
136 BICD2 23299
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
137 PRRC1 133619
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
138 SPAG5 10615
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
139 LAMTOR1 55004
Proximity Label-MS Homo sapiens
140 SMC1A 8243
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
141 HECTD1 25831
Affinity Capture-MS Homo sapiens
142 CSN1S1 1446
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
143 PAK7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
144 SLX4IP  
Affinity Capture-MS Homo sapiens
145 RFX3 5991
Affinity Capture-MS Homo sapiens
146 REST 5978
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
147 FZR1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
148 CYLD  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
149 PTPN14 5784
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
150 MPRIP 23164
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
151 CALML5 51806
Affinity Capture-Western Homo sapiens
152 CDC14A  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
153 PHLPP1  
Proximity Label-MS Homo sapiens
154 RECQL5  
Two-hybrid Homo sapiens
155 STK3 6788
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
156 SLX4  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
157 GTF3C3 9330
Affinity Capture-MS Homo sapiens
158 PSMA3 5684
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
159 HSF1  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
160 FTL 2512
Affinity Capture-MS Homo sapiens
161 BSND  
Affinity Capture-MS Homo sapiens
162 MUS81  
Affinity Capture-Western Homo sapiens
163 TP53BP1 7158
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
164 ANGPT1 284
Affinity Capture-MS Homo sapiens
165 RICTOR 253260
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
166 USP15 9958
Affinity Capture-MS Homo sapiens
167 CSN2 1447
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
168 RELA 5970
Reconstituted Complex Homo sapiens
169 WEE1 7465
Biochemical Activity Homo sapiens
170 C11orf85  
Affinity Capture-MS Homo sapiens
171 JADE1  
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
172 EPB41L4A 64097
Proximity Label-MS Homo sapiens
173 CTNNB1 1499
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
174 C1orf226  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
175 SREBF1 6720
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
176 PPP6C 5537
Affinity Capture-MS Homo sapiens
177 FEZ1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
178 PSMA2 5683
Affinity Capture-MS Homo sapiens
179 DAB2IP 153090
Affinity Capture-Western Homo sapiens
180 SGOL1  
Affinity Capture-Western Homo sapiens
181 STIM1 6786
Proximity Label-MS Homo sapiens
182 DNM2 1785
Affinity Capture-MS Homo sapiens
183 ERCC4  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
184 EGFR 1956
Affinity Capture-MS Homo sapiens
185 ZC3HC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
186 ARF6 382
Proximity Label-MS Homo sapiens
187 FBXO6 26270
Affinity Capture-MS Homo sapiens
188 NUAK1  
Affinity Capture-Western Homo sapiens
189 SNX27 81609
Affinity Capture-MS Homo sapiens
190 TP73  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
191 GTF3C4 9329
Affinity Capture-MS Homo sapiens
192 FLJ20712  
Two-hybrid Homo sapiens
193 CLSPN  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
194 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
195 PPID 5481
Two-hybrid Homo sapiens
196 PITPNA 5306
Affinity Capture-MS Homo sapiens
197 HMCES  
Affinity Capture-MS Homo sapiens
198 MAPRE3  
Proximity Label-MS Homo sapiens
199 KCNE3  
Affinity Capture-MS Homo sapiens
200 MAPK6  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
201 ANKRD52 283373
Affinity Capture-MS Homo sapiens
202 NOS1AP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
203 MCM4 4173
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
204 MYC  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
205 LDLR 3949
Negative Genetic Homo sapiens
206 ZCCHC6 79670
Affinity Capture-MS Homo sapiens
207 MRPL55  
Affinity Capture-MS Homo sapiens
208 MPLKIP  
Affinity Capture-MS Homo sapiens
209 PSMB5 5693
Affinity Capture-Western Homo sapiens
210 NDC80 10403
Co-fractionation Homo sapiens
211 VRK2 7444
Biochemical Activity Homo sapiens
212 Haus3  
Affinity Capture-MS Mus musculus
213 CUL7 9820
Affinity Capture-MS Homo sapiens
214 PTEN 5728
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
215 KAT8  
Affinity Capture-MS Homo sapiens
216 SF3B3 23450
Affinity Capture-MS Homo sapiens
217 NDUFA10 4705
Affinity Capture-MS Homo sapiens
218 PCM1 5108
Proximity Label-MS Homo sapiens
219 CDC20B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
220 MIER2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
221 HSPA9 3313
Affinity Capture-MS Homo sapiens
222 CPSF3L 54973
Two-hybrid Homo sapiens
223 PEX3 8504
Proximity Label-MS Homo sapiens
224 NFATC2  
Affinity Capture-MS Homo sapiens
225 ATP7A 538
Affinity Capture-MS Homo sapiens
226 PSMB1 5689
Affinity Capture-MS Homo sapiens
227 AKAP1 8165
Proximity Label-MS Homo sapiens
228 MDM2  
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
229 Plk1  
Affinity Capture-MS Mus musculus
230 DCTPP1 79077
Affinity Capture-MS Homo sapiens
231 RBBP8  
Affinity Capture-MS Homo sapiens
232 LMO4  
Two-hybrid Homo sapiens
233 SACS 26278
Affinity Capture-MS Homo sapiens
234 CEP97 79598
Affinity Capture-MS Homo sapiens
235 AHSA1 10598
Affinity Capture-MS Homo sapiens
236 SUGT1 10910
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
237 MAPKAPK2 9261
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
238 KIF24  
Affinity Capture-MS Homo sapiens
239 TRAK2 66008
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
240 TANK 10010
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
241 TP53BP2  
Two-hybrid Homo sapiens
242 FOXO3  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
243 CDC20 991
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
244 Ercc6l  
Affinity Capture-MS Mus musculus
245 FAM53C 51307
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
246 TRAK1 22906
Affinity Capture-MS Homo sapiens
247 CUL2 8453
Affinity Capture-Western Homo sapiens
248 TOPBP1 11073
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
249 PTPRD 5789
Reconstituted Complex Homo sapiens
250 VAMP4 8674
Affinity Capture-MS Homo sapiens
251 USP1 7398
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
252 MTMR3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
253 C9orf114  
Two-hybrid Homo sapiens
254 EVI5  
Affinity Capture-MS Homo sapiens
255 SLX1B  
Affinity Capture-MS Homo sapiens
256 PIN1 5300
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
257 PSMB7 5695
Affinity Capture-Western Homo sapiens
258 RPP25L  
Affinity Capture-MS Homo sapiens
259 MEN1 4221
Affinity Capture-MS Homo sapiens
260 TUBG1 7283
Affinity Capture-Western Homo sapiens
261 IKBKG 8517
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
262 NEK7 140609
Affinity Capture-MS Homo sapiens
263 RGPD4  
Affinity Capture-MS Homo sapiens
264 DNAAF5 54919
Affinity Capture-MS Homo sapiens
265 FBXL5  
Two-hybrid Homo sapiens
266 SQSTM1 8878
Proximity Label-MS Homo sapiens
267 DSC1 1823
Affinity Capture-MS Homo sapiens
268 ANKRD44 91526
Affinity Capture-MS Homo sapiens
269 SMC3 9126
Co-fractionation Homo sapiens
270 DCAF11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
271 CDC25C  
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
272 NUP155 9631
Proximity Label-MS Homo sapiens
273 C11orf74  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
274 GET4 51608
Two-hybrid Homo sapiens
275 NHSL2  
Two-hybrid Homo sapiens
276 TIMELESS  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
277 TOP2A 7153
Biochemical Activity Homo sapiens
278 SRPX 8406
Affinity Capture-MS Homo sapiens
279 BABAM1 29086
Affinity Capture-MS Homo sapiens
280 TBK1 29110
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
281 MCM3 4172
Affinity Capture-Western Homo sapiens
282 CEBPA  
Protein-peptide Homo sapiens
283 MCM5 4174
Affinity Capture-Western Homo sapiens
284 MAGED1 9500
Two-hybrid Homo sapiens
285 STXBP4 252983
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
286 PSMA5 5686
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
287 KRT8 3856
Proximity Label-MS Homo sapiens
288 LRRK2 120892
Reconstituted Complex Homo sapiens
289 PSMA7 5688
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
290 STAG2 10735
Biochemical Activity Homo sapiens
291 RXRA 6256
Two-hybrid Homo sapiens
292 CIC 23152
Two-hybrid Homo sapiens
293 IFT43  
Affinity Capture-MS Homo sapiens
294 RANBP2 5903
Affinity Capture-MS Homo sapiens
295 HNRNPF 3185
Affinity Capture-MS Homo sapiens
296 KLHL22 84861
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
297 TOPORS  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
298 SNW1 22938
Affinity Capture-MS Homo sapiens
299 KRAS 3845
Proximity Label-MS Homo sapiens
Negative Genetic Homo sapiens
300 C9orf72  
Affinity Capture-MS Homo sapiens
301 ERCC6L 54821
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
302 PHC2  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
303 NEFM 4741
Proximity Label-MS Homo sapiens
304 OSBPL6  
Affinity Capture-MS Homo sapiens
305 IVNS1ABP  
Affinity Capture-MS Homo sapiens
306 Nme7  
Affinity Capture-MS Mus musculus
307 IKBKB 3551
Biochemical Activity Homo sapiens
308 RPA3 6119
Proximity Label-MS Homo sapiens
309 PTHLH  
Affinity Capture-MS Homo sapiens
310 KLHL21 9903
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
311 DNHD1  
Biochemical Activity Homo sapiens
312 ORC3  
Affinity Capture-MS Homo sapiens
313 USP20  
Affinity Capture-MS Homo sapiens
314 CDC5L 988
Affinity Capture-MS Homo sapiens
315 ATP5A1 498
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PLK1 is involved
PathwayEvidenceSource
Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins TAS Reactome
Activation of NIMA Kinases NEK9, NEK6, NEK7 TAS Reactome
Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal TAS Reactome
Amplification of signal from the kinetochores TAS Reactome
Anchoring of the basal body to the plasma membrane TAS Reactome
APC/C-mediated degradation of cell cycle proteins TAS Reactome
APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 TAS Reactome
AURKA Activation by TPX2 TAS Reactome
Cell Cycle TAS Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints TAS Reactome
Cell Cycle, Mitotic TAS Reactome
Cell Cycle, Mitotic IEA Reactome
Centrosome maturation TAS Reactome
Cilium Assembly TAS Reactome
Condensation of Prophase Chromosomes TAS Reactome
Cyclin A/B1/B2 associated events during G2/M transition TAS Reactome
Developmental Biology TAS Reactome
EML4 and NUDC in mitotic spindle formation TAS Reactome
G2/M Transition TAS Reactome
Golgi Cisternae Pericentriolar Stack Reorganization TAS Reactome
Golgi Cisternae Pericentriolar Stack Reorganization IEA Reactome
Loss of Nlp from mitotic centrosomes TAS Reactome
Loss of proteins required for interphase microtubule organization from the centrosome TAS Reactome
M Phase TAS Reactome
M Phase IEA Reactome
MITF-M-dependent gene expression TAS Reactome
MITF-M-regulated melanocyte development TAS Reactome
Mitotic Anaphase TAS Reactome
Mitotic G2-G2/M phases TAS Reactome
Mitotic Metaphase and Anaphase TAS Reactome
Mitotic Metaphase/Anaphase Transition TAS Reactome
Mitotic Prometaphase TAS Reactome
Mitotic Prophase TAS Reactome
Mitotic Prophase IEA Reactome
Mitotic Spindle Checkpoint TAS Reactome
Mitotic Telophase/Cytokinesis TAS Reactome
Nuclear Envelope Breakdown TAS Reactome
Organelle biogenesis and maintenance TAS Reactome
Phosphorylation of Emi1 TAS Reactome
Phosphorylation of the APC/C TAS Reactome
Polo-like kinase mediated events TAS Reactome
Recruitment of mitotic centrosome proteins and complexes TAS Reactome
Recruitment of NuMA to mitotic centrosomes TAS Reactome
Regulation of APC/C activators between G1/S and early anaphase TAS Reactome
Regulation of MITF-M-dependent genes involved in cell cycle and proliferation TAS Reactome
Regulation of mitotic cell cycle TAS Reactome
Regulation of PLK1 Activity at G2/M Transition TAS Reactome
Resolution of Sister Chromatid Cohesion TAS Reactome
RHO GTPase Effectors TAS Reactome
RHO GTPases Activate Formins TAS Reactome
Separation of Sister Chromatids TAS Reactome
Signal Transduction TAS Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
The role of GTSE1 in G2/M progression after G2 checkpoint TAS Reactome





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