Gene description for SNCB
Gene name synuclein, beta
Gene symbol SNCB
Other names/aliases -
Species Homo sapiens
 Database cross references - SNCB
ExoCarta ExoCarta_6620
Vesiclepedia VP_6620
Entrez Gene 6620
HGNC 11140
MIM 602569
UniProt Q16143  
 SNCB identified in sEVs derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for SNCB
Molecular Function
    phospholipase inhibitor activity GO:0004859 TAS
    calcium ion binding GO:0005509 IDA
    protein binding GO:0005515 IPI
    transition metal ion binding GO:0046914 IMP
    cuprous ion binding GO:1903136 IBA
    cuprous ion binding GO:1903136 IMP
Biological Process
    chemical synaptic transmission GO:0007268 IBA
    dopamine metabolic process GO:0042417 IEA
    negative regulation of neuron apoptotic process GO:0043524 IEA
    synaptic vesicle endocytosis GO:0048488 IBA
    synapse organization GO:0050808 IBA
    neuron apoptotic process GO:0051402 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    inclusion body GO:0016234 IDA
    neuronal cell body GO:0043025 IBA
    axon terminus GO:0043679 IBA
 Experiment description of studies that identified SNCB in sEVs
1
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SNCB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APP 351
Reconstituted Complex Homo sapiens
2 ARMC1 55156
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 Tubb3 22152
Co-purification Mus musculus
4 GRK5 2869
Biochemical Activity Homo sapiens
5 ADRBK1 282682
Biochemical Activity Bos taurus
6 Tuba1a 22142
Co-purification Mus musculus
7 DNAAF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 SNCA 6622
Affinity Capture-Western Homo sapiens
9 KIF20A 10112
Affinity Capture-MS Homo sapiens
10 GRK6 2870
Biochemical Activity Homo sapiens
11 PLK3  
Biochemical Activity Homo sapiens
12 LNX1  
Reconstituted Complex Homo sapiens
13 HSPA12A 259217
Affinity Capture-MS Homo sapiens
14 NUFIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 PLK1 5347
Biochemical Activity Homo sapiens
16 GRK1  
Biochemical Activity Homo sapiens
17 DGUOK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 HSPA1A 3303
FRET Homo sapiens
View the network image/svg+xml
 Pathways in which SNCB is involved
PathwayEvidenceSource
Cellular responses to stimuli TAS Reactome
MTF1 activates gene expression TAS Reactome
Response to metal ions TAS Reactome





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