Gene description for NEK7
Gene name NIMA-related kinase 7
Gene symbol NEK7
Other names/aliases -
Species Homo sapiens
 Database cross references - NEK7
ExoCarta ExoCarta_140609
Vesiclepedia VP_140609
Entrez Gene 140609
HGNC 13386
MIM 606848
UniProt Q8TDX7  
 NEK7 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Endothelial cells 26027894    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for NEK7
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    metal ion binding GO:0046872 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    molecular function activator activity GO:0140677 IDA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    regulation of mitotic cell cycle GO:0007346 IDA
    regulation of mitotic cell cycle GO:0007346 TAS
    positive regulation of telomere maintenance GO:0032206 IMP
    positive regulation of telomere maintenance via telomerase GO:0032212 IBA
    cellular response to potassium ion GO:0035865 ISS
    spindle assembly GO:0051225 TAS
    positive regulation of NLRP3 inflammasome complex assembly GO:1900227 IBA
    positive regulation of NLRP3 inflammasome complex assembly GO:1900227 IDA
Subcellular Localization
    spindle pole GO:0000922 IEA
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 ISS
    centrosome GO:0005813 IEA
    microtubule organizing center GO:0005815 IDA
    microtubule GO:0005874 IEA
 Experiment description of studies that identified NEK7 in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 226
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
6
Experiment ID 488
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 217
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NEK7
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CBS 875
Affinity Capture-MS Homo sapiens
2 DPH3  
Affinity Capture-MS Homo sapiens
3 LLGL2 3993
Affinity Capture-MS Homo sapiens
4 SH3PXD2B 285590
Co-fractionation Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 PCDHGB1  
Affinity Capture-MS Homo sapiens
7 TMEM9 252839
Affinity Capture-MS Homo sapiens
8 LAMP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 EML4 27436
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
10 NEK6 10783
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 SCYL1 57410
Affinity Capture-MS Homo sapiens
12 ZNF669  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 PYCARD 29108
Affinity Capture-Western Homo sapiens
14 P4HA2 8974
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 NFATC1 4772
Affinity Capture-MS Homo sapiens
16 CASP1  
Affinity Capture-Western Homo sapiens
17 TNF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 CMPK1 51727
Co-fractionation Homo sapiens
19 SLC9A3R1 9368
Co-fractionation Homo sapiens
20 CUL3 8452
Affinity Capture-MS Homo sapiens
21 PDCD2L 84306
Affinity Capture-MS Homo sapiens
22 GPR107 57720
Affinity Capture-MS Homo sapiens
23 SLC18A2  
Affinity Capture-MS Homo sapiens
24 WDR35 57539
Affinity Capture-MS Homo sapiens
25 FILIP1  
Affinity Capture-MS Homo sapiens
26 PLK1 5347
Affinity Capture-MS Homo sapiens
27 EHD2 30846
Affinity Capture-MS Homo sapiens
28 PALB2  
Affinity Capture-MS Homo sapiens
29 SLC39A9 55334
Affinity Capture-MS Homo sapiens
30 EML3 256364
Affinity Capture-MS Homo sapiens
31 ANKS6 203286
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 RAET1L  
Affinity Capture-MS Homo sapiens
33 ZNF556 80032
Affinity Capture-MS Homo sapiens
34 SULF2 55959
Affinity Capture-MS Homo sapiens
35 IL13RA2 3598
Affinity Capture-MS Homo sapiens
36 RPA3 6119
Proximity Label-MS Homo sapiens
37 NLRP3  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
38 PTPN21 11099
Affinity Capture-MS Homo sapiens
39 ZNF214  
Affinity Capture-MS Homo sapiens
40 NEK8  
Affinity Capture-MS Homo sapiens
41 ANKS3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 RREB1 6239
Affinity Capture-MS Homo sapiens
43 APLNR  
Affinity Capture-MS Homo sapiens
44 P4HB 5034
Affinity Capture-MS Homo sapiens
45 IFT122 55764
Affinity Capture-MS Homo sapiens
46 NEK9 91754
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 ZNF331  
Affinity Capture-MS Homo sapiens
48 WDR19 57728
Affinity Capture-MS Homo sapiens
49 DYNLL1 8655
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 Nek9  
Affinity Capture-MS Mus musculus
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