Gene description for AFAP1L1
Gene name actin filament associated protein 1-like 1
Gene symbol AFAP1L1
Other names/aliases -
Species Homo sapiens
 Database cross references - AFAP1L1
ExoCarta ExoCarta_134265
Vesiclepedia VP_134265
Entrez Gene 134265
HGNC 26714
MIM 614410
UniProt Q8TED9  
 AFAP1L1 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for AFAP1L1
Molecular Function
    protein binding GO:0005515 IPI
Subcellular Localization
    stress fiber GO:0001725 IEA
    podosome GO:0002102 IEA
    cytosol GO:0005829 IBA
    cell projection GO:0042995 IEA
    anchoring junction GO:0070161 IEA
 Experiment description of studies that identified AFAP1L1 in sEVs
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for AFAP1L1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GADD45G  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
2 DECR1 1666
Affinity Capture-MS Homo sapiens
3 YWHAE 7531
Affinity Capture-MS Homo sapiens
4 KLHL42  
Affinity Capture-MS Homo sapiens
5 TRIP6 7205
Affinity Capture-MS Homo sapiens
6 PTOV1  
Affinity Capture-MS Homo sapiens
7 YWHAG 7532
Affinity Capture-MS Homo sapiens
8 DDX3Y 8653
Affinity Capture-MS Homo sapiens
9 KLHDC2  
Affinity Capture-MS Homo sapiens
10 KDM4A  
Affinity Capture-MS Homo sapiens
11 FEM1B 10116
Affinity Capture-MS Homo sapiens
12 YWHAB 7529
Affinity Capture-MS Homo sapiens
13 HSD17B1  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which AFAP1L1 is involved
No pathways found





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