Gene description for NOD1
Gene name nucleotide-binding oligomerization domain containing 1
Gene symbol NOD1
Other names/aliases CARD4
CLR7.1
NLRC1
Species Homo sapiens
 Database cross references - NOD1
ExoCarta ExoCarta_10392
Vesiclepedia VP_10392
Entrez Gene 10392
HGNC 16390
MIM 605980
UniProt Q9Y239  
 NOD1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for NOD1
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    cysteine-type endopeptidase activator activity involved in apoptotic process GO:0008656 TAS
    pattern recognition receptor activity GO:0038187 IDA
    identical protein binding GO:0042802 IDA
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IPI
    peptidoglycan binding GO:0042834 IDA
    peptidoglycan binding GO:0042834 TAS
    ubiquitin binding GO:0043130 IDA
    protein-containing complex binding GO:0044877 IPI
    CARD domain binding GO:0050700 IDA
Biological Process
    pattern recognition receptor signaling pathway GO:0002221 IDA
    positive regulation of dendritic cell antigen processing and presentation GO:0002606 ISS
    apoptotic process GO:0006915 IEA
    defense response GO:0006952 TAS
    inflammatory response GO:0006954 TAS
    signal transduction GO:0007165 TAS
    JNK cascade GO:0007254 IEA
    detection of biotic stimulus GO:0009595 TAS
    detection of bacterium GO:0016045 IDA
    positive regulation of interleukin-1 beta production GO:0032731 IEA
    positive regulation of interleukin-6 production GO:0032755 IEA
    positive regulation of interleukin-8 production GO:0032757 IDA
    positive regulation of tumor necrosis factor production GO:0032760 IEA
    positive regulation of stress-activated MAPK cascade GO:0032874 IEA
    response to endoplasmic reticulum stress GO:0034976 IEA
    intracellular signal transduction GO:0035556 IBA
    intracellular signal transduction GO:0035556 IDA
    defense response to bacterium GO:0042742 IDA
    positive regulation of apoptotic process GO:0043065 IEA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IDA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IDA
    innate immune response GO:0045087 IEA
    positive regulation of JNK cascade GO:0046330 IEA
    defense response to Gram-negative bacterium GO:0050829 IDA
    defense response to Gram-positive bacterium GO:0050830 IEA
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IDA
    stress-activated MAPK cascade GO:0051403 IEA
    positive regulation of macrophage cytokine production GO:0060907 IEA
    ERK1 and ERK2 cascade GO:0070371 IEA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IEA
    nucleotide-binding oligomerization domain containing 1 signaling pathway GO:0070427 IDA
    cellular response to muramyl dipeptide GO:0071225 IMP
    xenophagy GO:0098792 IEA
    positive regulation of non-canonical NF-kappaB signal transduction GO:1901224 IMP
    positive regulation of xenophagy GO:1904417 IEA
    positive regulation of cysteine-type endopeptidase activity GO:2001056 IDA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 TAS
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IMP
    basolateral plasma membrane GO:0016323 IDA
    apical plasma membrane GO:0016324 IEA
    phagocytic vesicle GO:0045335 IEA
 Experiment description of studies that identified NOD1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NOD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATG16L1 55054
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
2 CASP4  
Affinity Capture-Western Homo sapiens
3 RIPK2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 NOD1 10392
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
5 NLRC4  
Affinity Capture-Western Homo sapiens
6 SOX8  
Two-hybrid Homo sapiens
7 GPS1 2873
Affinity Capture-Western Homo sapiens
8 XIAP  
Reconstituted Complex Homo sapiens
9 IRGM 345611
Affinity Capture-Western Homo sapiens
10 RNF34  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
11 TRAF4 9618
Affinity Capture-Western Homo sapiens
12 SUGT1 10910
Affinity Capture-Western Homo sapiens
13 SREBF1 6720
Negative Genetic Homo sapiens
14 CASP8 841
Affinity Capture-Western Homo sapiens
15 CASP2  
Affinity Capture-Western Homo sapiens
16 UBC 7316
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
17 HSP90AA1 3320
Affinity Capture-Western Homo sapiens
18 SHMT2 6472
Affinity Capture-RNA Homo sapiens
19 CARD6  
Affinity Capture-Western Homo sapiens
20 NOD2 64127
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 CASP1  
Affinity Capture-Western Homo sapiens
22 MOV10 4343
Affinity Capture-RNA Homo sapiens
23 PSMA7 5688
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
24 COPS6 10980
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 CASP9 842
Affinity Capture-Western Homo sapiens
26 CFLAR  
Affinity Capture-Western Homo sapiens
27 GABRB1  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which NOD1 is involved
PathwayEvidenceSource
activated TAK1 mediates p38 MAPK activation TAS Reactome
Cytokine Signaling in Immune system TAS Reactome
Deubiquitination TAS Reactome
Disease TAS Reactome
Immune System TAS Reactome
Immune System IEA Reactome
Infectious disease TAS Reactome
Innate Immune System TAS Reactome
Innate Immune System IEA Reactome
Interleukin-1 family signaling TAS Reactome
Interleukin-1 signaling TAS Reactome
Interleukin-17 signaling TAS Reactome
JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1 TAS Reactome
MAP kinase activation TAS Reactome
Metabolism of proteins TAS Reactome
MyD88 cascade initiated on plasma membrane TAS Reactome
MyD88 dependent cascade initiated on endosome TAS Reactome
MyD88-independent TLR4 cascade TAS Reactome
MyD88:MAL(TIRAP) cascade initiated on plasma membrane TAS Reactome
NOD1/2 Signaling Pathway TAS Reactome
NOD1/2 Signaling Pathway IEA Reactome
Nucleotide-binding domain, leucine rich repeat containing receptor (NLR) signaling pathways TAS Reactome
Nucleotide-binding domain, leucine rich repeat containing receptor (NLR) signaling pathways IEA Reactome
Ovarian tumor domain proteases TAS Reactome
Post-translational protein modification TAS Reactome
SARS-CoV Infections TAS Reactome
SARS-CoV-2 activates/modulates innate and adaptive immune responses TAS Reactome
SARS-CoV-2 Infection TAS Reactome
SARS-CoV-2-host interactions TAS Reactome
Signaling by Interleukins TAS Reactome
TAK1-dependent IKK and NF-kappa-B activation TAS Reactome
Toll Like Receptor 10 (TLR10) Cascade TAS Reactome
Toll Like Receptor 2 (TLR2) Cascade TAS Reactome
Toll Like Receptor 3 (TLR3) Cascade TAS Reactome
Toll Like Receptor 4 (TLR4) Cascade TAS Reactome
Toll Like Receptor 5 (TLR5) Cascade TAS Reactome
Toll Like Receptor 7/8 (TLR7/8) Cascade TAS Reactome
Toll Like Receptor 9 (TLR9) Cascade TAS Reactome
Toll Like Receptor TLR1:TLR2 Cascade TAS Reactome
Toll Like Receptor TLR6:TLR2 Cascade TAS Reactome
Toll-like Receptor Cascades TAS Reactome
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation TAS Reactome
TRIF (TICAM1)-mediated TLR4 signaling TAS Reactome
Viral Infection Pathways TAS Reactome





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