Gene description for CHKA
Gene name choline kinase alpha
Gene symbol CHKA
Other names/aliases CHK
CK
CKI
EK
Species Homo sapiens
 Database cross references - CHKA
ExoCarta ExoCarta_1119
Entrez Gene 1119
HGNC 1937
MIM 118491
UniProt P35790  
 CHKA identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for CHKA
Molecular Function
    choline kinase activity GO:0004103 IDA
    choline binding GO:0033265 IEA
    ATP binding GO:0005524 IEA
    signal transducer activity GO:0004871 TAS
    cholinesterase activity GO:0004104 IEA
    protein homodimerization activity GO:0042803 IEA
    ethanolamine kinase activity GO:0004305 IDA
    drug binding GO:0008144 IDA
Biological Process
    signal transduction GO:0007165 TAS
    phosphatidylethanolamine biosynthetic process GO:0006646 TAS
    lipid metabolic process GO:0006629 TAS
    CDP-choline pathway GO:0006657 TAS
    phosphorylation GO:0016310 IEA
    phosphatidylcholine biosynthetic process GO:0006656 TAS
    lipid transport GO:0006869 TAS
    glycerophospholipid biosynthetic process GO:0046474 TAS
    phospholipid metabolic process GO:0006644 TAS
    choline metabolic process GO:0019695 IEA
    small molecule metabolic process GO:0044281 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
 Experiment description of studies that identified CHKA in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for CHKA
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which CHKA is involved
PathwayEvidenceSource
Synthesis of PC TAS Reactome
Synthesis of PE TAS Reactome





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