Gene description for WDFY1
Gene name WD repeat and FYVE domain containing 1
Gene symbol WDFY1
Other names/aliases FENS-1
WDF1
ZFYVE17
Species Homo sapiens
 Database cross references - WDFY1
ExoCarta ExoCarta_57590
Vesiclepedia VP_57590
Entrez Gene 57590
HGNC 20451
UniProt Q8IWB7  
 WDFY1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Endothelial cells 26027894    
Thymus 23844026    
 Gene ontology annotations for WDFY1
Molecular Function
    protein binding GO:0005515 IPI
    1-phosphatidylinositol binding GO:0005545 IDA
    zinc ion binding GO:0008270 NAS
Biological Process
    positive regulation of toll-like receptor 3 signaling pathway GO:0034141 IBA
    positive regulation of toll-like receptor 3 signaling pathway GO:0034141 IMP
    positive regulation of toll-like receptor 4 signaling pathway GO:0034145 IBA
    positive regulation of toll-like receptor 4 signaling pathway GO:0034145 IMP
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleolus GO:0005730 IDA
    early endosome GO:0005769 IBA
    early endosome GO:0005769 IDA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IDA
    cell junction GO:0030054 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified WDFY1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 226
MISEV standards
EM
Biophysical techniques
GAPDH|CD9|FLOT1
Enriched markers
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
6
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for WDFY1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 COX5B 1329
Co-fractionation Homo sapiens
2 SNX1 6642
Co-fractionation Homo sapiens
3 EHD1 10938
Co-fractionation Homo sapiens
4 HACL1 26061
Co-fractionation Homo sapiens
5 GNG2 54331
Affinity Capture-MS Homo sapiens
6 ALDOC 230
Co-fractionation Homo sapiens
7 MYLIP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 SCAMP2 10066
Affinity Capture-MS Homo sapiens
9 LHX6  
Affinity Capture-MS Homo sapiens
10 VPS28 51160
Affinity Capture-MS Homo sapiens
11 EGR1  
Affinity Capture-MS Homo sapiens
12 L3HYPDH 112849
Co-fractionation Homo sapiens
13 VAMP8 8673
Affinity Capture-MS Homo sapiens
14 ATG9A 79065
Proximity Label-MS Homo sapiens
15 TPD52L2 7165
Co-fractionation Homo sapiens
16 S1PR2 9294
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 SCAMP1 9522
Affinity Capture-MS Homo sapiens
18 SNX2 6643
Co-fractionation Homo sapiens
19 C19orf66  
Affinity Capture-MS Homo sapiens
20 VAMP3 9341
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 SQSTM1 8878
Proximity Label-MS Homo sapiens
22 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
23 TMEM123 114908
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 NARFL 64428
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 OSGEP 55644
Affinity Capture-MS Homo sapiens
26 CCDC88C 440193
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 ATG14  
Affinity Capture-MS Homo sapiens
28 SIRT5 23408
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 SEPT2 4735
Affinity Capture-MS Homo sapiens
30 VAMP2 6844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 HMMR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 FAM219A 203259
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 PARK2  
Affinity Capture-MS Homo sapiens
34 NUDT1  
Affinity Capture-MS Homo sapiens
35 RPA3 6119
Proximity Label-MS Homo sapiens
36 SEC31A 22872
Co-fractionation Homo sapiens
37 CCNB2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 SEPT11 55752
Affinity Capture-MS Homo sapiens
39 TLR3  
Affinity Capture-Western Homo sapiens
40 TSPAN6 7105
Affinity Capture-MS Homo sapiens
41 RAB11A 8766
Affinity Capture-MS Homo sapiens
42 C16orf45  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 RAB7A 7879
Affinity Capture-MS Homo sapiens
44 FH 2271
Co-fractionation Homo sapiens
45 GDI1 2664
Co-fractionation Homo sapiens
46 STX7 8417
Affinity Capture-MS Homo sapiens
47 STBD1 8987
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 KCTD21 283219
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 GPR37  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 UBQLN1 29979
Co-fractionation Homo sapiens
51 TXNL4A 10907
Co-fractionation Homo sapiens
52 FAHD2A 51011
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which WDFY1 is involved
No pathways found





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