Gene description for MTHFR
Gene name methylenetetrahydrofolate reductase (NAD(P)H)
Gene symbol MTHFR
Other names/aliases -
Species Homo sapiens
 Database cross references - MTHFR
ExoCarta ExoCarta_4524
Vesiclepedia VP_4524
Entrez Gene 4524
HGNC 7436
MIM 607093
UniProt P42898  
 MTHFR identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for MTHFR
Molecular Function
    methylenetetrahydrofolate reductase (NAD(P)H) activity GO:0004489 IBA
    methylenetetrahydrofolate reductase (NAD(P)H) activity GO:0004489 IDA
    methylenetetrahydrofolate reductase (NAD(P)H) activity GO:0004489 IGI
    methylenetetrahydrofolate reductase (NAD(P)H) activity GO:0004489 IMP
    protein binding GO:0005515 IPI
    protein-containing complex binding GO:0044877 IPI
    flavin adenine dinucleotide binding GO:0050660 IDA
    NADP binding GO:0050661 IEA
    FAD binding GO:0071949 IBA
    modified amino acid binding GO:0072341 IDA
    methylenetetrahydrofolate reductase (NADPH) activity GO:0106313 IEA
Biological Process
    response to hypoxia GO:0001666 IEA
    neural tube closure GO:0001843 IMP
    neural tube closure GO:0001843 NAS
    methionine metabolic process GO:0006555 IGI
    methionine biosynthetic process GO:0009086 IBA
    response to xenobiotic stimulus GO:0009410 IEA
    response to vitamin B2 GO:0033274 IEA
    tetrahydrofolate interconversion GO:0035999 IBA
    tetrahydrofolate interconversion GO:0035999 IDA
    tetrahydrofolate interconversion GO:0035999 IEA
    tetrahydrofolate interconversion GO:0035999 IGI
    tetrahydrofolate interconversion GO:0035999 IMP
    response to amino acid GO:0043200 IEA
    S-adenosylmethionine metabolic process GO:0046500 IEA
    homocysteine metabolic process GO:0050667 IDA
    response to folic acid GO:0051593 IEA
    response to interleukin-1 GO:0070555 IEA
    heterochromatin organization GO:0070828 IDA
Subcellular Localization
    cytosol GO:0005829 TAS
 Experiment description of studies that identified MTHFR in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MTHFR
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 C6orf222  
Affinity Capture-MS Homo sapiens
2 HSD3B2 3284
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 TLR10  
Affinity Capture-MS Homo sapiens
4 CDCA8 55143
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 GNAZ 2781
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 G0S2  
Affinity Capture-MS Homo sapiens
7 ANKLE2 23141
Affinity Capture-MS Homo sapiens
8 LSM8 51691
Two-hybrid Homo sapiens
9 SPATA19  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 COX4I2 84701
Affinity Capture-MS Homo sapiens
11 CXorf66  
Affinity Capture-MS Homo sapiens
12 TYROBP  
Affinity Capture-MS Homo sapiens
13 TMEM223  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 UBA52 7311
Affinity Capture-MS Homo sapiens
15 KPNA6 23633
Affinity Capture-MS Homo sapiens
16 TIGD6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 RASSF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 BCL2L11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 DCLRE1C  
Affinity Capture-MS Homo sapiens
20 KCNF1  
Affinity Capture-MS Homo sapiens
21 SIRT1  
Affinity Capture-MS Homo sapiens
22 KCNV2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 TLR9  
Affinity Capture-MS Homo sapiens
24 BTN3A1 11119
Affinity Capture-MS Homo sapiens
25 ZNF556 80032
Affinity Capture-MS Homo sapiens
26 NOX5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 BCL2  
Affinity Capture-MS Homo sapiens
28 SP110  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 C18orf25 147339
Affinity Capture-MS Homo sapiens
30 CUL4A 8451
Affinity Capture-MS Homo sapiens
31 SMPD2 6610
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 TULP3 7289
Affinity Capture-MS Homo sapiens
33 PCDHB16  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 GDF10 2662
Affinity Capture-MS Homo sapiens
35 SYT2  
Affinity Capture-MS Homo sapiens
36 PRPF31 26121
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 EPHA1 2041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 ZNF263  
Affinity Capture-MS Homo sapiens
39 GNAI1 2770
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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