Gene description for RAPH1
Gene name Ras association (RalGDS/AF-6) and pleckstrin homology domains 1
Gene symbol RAPH1
Other names/aliases ALS2CR18
ALS2CR9
LPD
PREL-2
PREL2
RMO1
RalGDS/AF-6
Species Homo sapiens
 Database cross references - RAPH1
ExoCarta ExoCarta_65059
Entrez Gene 65059
HGNC 14436
MIM 609035
UniProt Q70E73  
 RAPH1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for RAPH1
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    cell-matrix adhesion GO:0007160 IEA
    axon extension GO:0048675 IEA
    signal transduction GO:0007165 IEA
Subcellular Localization
    lamellipodium GO:0030027 IEA
    cytoplasm GO:0005737 IEA
    cytoskeleton GO:0005856 IEA
    plasma membrane GO:0005886 IEA
    filopodium GO:0030175 IEA
 Experiment description of studies that identified RAPH1 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for RAPH1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ENAH 55740
Affinity Capture-Western Homo sapiens
2 VASP 7408
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which RAPH1 is involved
No pathways found





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