Gene description for CISD1
Gene name CDGSH iron sulfur domain 1
Gene symbol CISD1
Other names/aliases C10orf70
MDS029
ZCD1
mitoNEET
Species Homo sapiens
 Database cross references - CISD1
ExoCarta ExoCarta_55847
Vesiclepedia VP_55847
Entrez Gene 55847
HGNC 30880
MIM 611932
UniProt Q9NZ45  
 CISD1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for CISD1
Molecular Function
    pyridoxal phosphate binding GO:0030170 IDA
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IDA
    metal ion binding GO:0046872 IEA
    L-cysteine transaminase activity GO:0047801 IBA
    L-cysteine transaminase activity GO:0047801 IDA
    2 iron, 2 sulfur cluster binding GO:0051537 IBA
    2 iron, 2 sulfur cluster binding GO:0051537 IDA
Biological Process
    intracellular iron ion homeostasis GO:0006879 IBA
    regulation of autophagy GO:0010506 IEA
    regulation of cellular respiration GO:0043457 IEA
    protein maturation by [2Fe-2S] cluster transfer GO:0106034 IDA
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 ISS
    mitochondrial outer membrane GO:0005741 IBA
 Experiment description of studies that identified CISD1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CISD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STX12 23673
Co-fractionation Homo sapiens
2 TOMM40 10452
Co-fractionation Homo sapiens
3 CLTC 1213
Co-fractionation Homo sapiens
4 ATP5H 10476
Co-fractionation Homo sapiens
5 PDLIM7 9260
Affinity Capture-MS Homo sapiens
6 MAVS 57506
Proximity Label-MS Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 SPRTN  
Affinity Capture-MS Homo sapiens
9 MTCH1 23787
Proximity Label-MS Homo sapiens
10 WDR45  
Affinity Capture-MS Homo sapiens
11 BCAP31 10134
Co-fractionation Homo sapiens
12 AIFM1 9131
Proximity Label-MS Homo sapiens
13 RAB7A 7879
Proximity Label-MS Homo sapiens
14 KIF14 9928
Affinity Capture-MS Homo sapiens
15 MARCKS 4082
Proximity Label-MS Homo sapiens
16 MTX1 4580
Co-fractionation Homo sapiens
17 RPL6 6128
Co-fractionation Homo sapiens
18 PXMP2  
Proximity Label-MS Homo sapiens
19 ATP6V1C1 528
Co-fractionation Homo sapiens
20 RAB11A 8766
Affinity Capture-MS Homo sapiens
21 PPIB 5479
Co-fractionation Homo sapiens
22 MTCH2 23788
Proximity Label-MS Homo sapiens
23 SNRPA 6626
Two-hybrid Homo sapiens
24 MGARP  
Affinity Capture-MS Homo sapiens
25 CHCHD2  
Affinity Capture-MS Homo sapiens
26 NDUFA12 55967
Co-fractionation Homo sapiens
27 FKBP8 23770
Proximity Label-MS Homo sapiens
28 PARK2  
Affinity Capture-MS Homo sapiens
29 NDUFA4 4697
Affinity Capture-MS Homo sapiens
30 TSPO 706
Co-fractionation Homo sapiens
31 PSMD7 5713
Co-fractionation Homo sapiens
32 TTC30B  
Affinity Capture-MS Homo sapiens
33 OCIAD1 54940
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
34 PDE2A  
Affinity Capture-MS Homo sapiens
35 VSIG4  
Affinity Capture-MS Homo sapiens
36 KIAA1429 25962
Affinity Capture-MS Homo sapiens
37 ANXA11 311
Co-fractionation Homo sapiens
38 FIS1 51024
Proximity Label-MS Homo sapiens
39 NDUFA2 4695
Co-fractionation Homo sapiens
40 RPA3 6119
Proximity Label-MS Homo sapiens
41 COQ9  
Affinity Capture-MS Homo sapiens
42 SDHB 6390
Co-fractionation Homo sapiens
43 PTPMT1 114971
Affinity Capture-MS Homo sapiens
44 OPA1 4976
Proximity Label-MS Homo sapiens
45 CISD2 493856
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 STX7 8417
Co-fractionation Homo sapiens
47 RNF2  
Affinity Capture-MS Homo sapiens
48 C9orf72  
Affinity Capture-MS Homo sapiens
49 VDAC1 7416
Co-fractionation Homo sapiens
50 TOMM7 54543
Co-fractionation Homo sapiens
51 VDAC3 7419
Co-fractionation Homo sapiens
52 TECR 9524
Co-fractionation Homo sapiens
53 ABCE1 6059
Affinity Capture-MS Homo sapiens
54 RHOT2 89941
Proximity Label-MS Homo sapiens
55 ATP2A3 489
Affinity Capture-MS Homo sapiens
56 VDAC2 7417
Co-fractionation Homo sapiens
57 AKAP1 8165
Proximity Label-MS Homo sapiens
58 EXD2  
Proximity Label-MS Homo sapiens
59 RMDN3 55177
Proximity Label-MS Homo sapiens
60 SLC25A46 91137
Proximity Label-MS Homo sapiens
61 COX4I1 1327
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which CISD1 is involved
No pathways found





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