Gene description for Snrpd3
Gene name small nuclear ribonucleoprotein D3
Gene symbol Snrpd3
Other names/aliases 1700043E15Rik
2310009E13Rik
AW046420
SMD3
Species Mus musculus
 Database cross references - Snrpd3
ExoCarta ExoCarta_67332
Vesiclepedia VP_67332
Entrez Gene 67332
UniProt P62320  
 Snrpd3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Neural stem cells 25242146    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Snrpd3
Molecular Function
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 ISO
    enzyme binding GO:0019899 IEA
    enzyme binding GO:0019899 ISO
    telomerase RNA binding GO:0070034 IEA
    telomerase RNA binding GO:0070034 ISO
    histone pre-mRNA DCP binding GO:0071208 IDA
    U7 snRNA binding GO:0071209 IEA
    U7 snRNA binding GO:0071209 ISO
    U1 snRNP binding GO:1990446 ISO
Biological Process
    spliceosomal snRNP assembly GO:0000387 IBA
    spliceosomal snRNP assembly GO:0000387 ISO
    spliceosomal snRNP assembly GO:0000387 ISS
    mRNA splicing, via spliceosome GO:0000398 ISO
    mRNA splicing, via spliceosome GO:0000398 ISS
    protein methylation GO:0006479 ISO
Subcellular Localization
    commitment complex GO:0000243 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    spliceosomal complex GO:0005681 ISO
    U5 snRNP GO:0005682 IBA
    U7 snRNP GO:0005683 ISO
    U7 snRNP GO:0005683 ISS
    U2-type spliceosomal complex GO:0005684 ISO
    U2-type spliceosomal complex GO:0005684 ISS
    U1 snRNP GO:0005685 IBA
    U1 snRNP GO:0005685 ISO
    U1 snRNP GO:0005685 ISS
    U2 snRNP GO:0005686 IBA
    U4 snRNP GO:0005687 IBA
    U4 snRNP GO:0005687 ISO
    U4 snRNP GO:0005687 ISS
    U12-type spliceosomal complex GO:0005689 IEA
    U12-type spliceosomal complex GO:0005689 ISO
    telomerase holoenzyme complex GO:0005697 IEA
    telomerase holoenzyme complex GO:0005697 ISO
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    nuclear body GO:0016604 IEA
    nuclear body GO:0016604 ISO
    methylosome GO:0034709 ISO
    methylosome GO:0034709 ISS
    pICln-Sm protein complex GO:0034715 ISO
    pICln-Sm protein complex GO:0034715 ISS
    SMN-Sm protein complex GO:0034719 IBA
    SMN-Sm protein complex GO:0034719 ISO
    SMN-Sm protein complex GO:0034719 ISS
    U4/U6 x U5 tri-snRNP complex GO:0046540 ISO
    U4/U6 x U5 tri-snRNP complex GO:0046540 ISS
    U2-type precatalytic spliceosome GO:0071005 ISO
    U2-type precatalytic spliceosome GO:0071005 ISS
    U2-type catalytic step 2 spliceosome GO:0071007 ISO
    U2-type catalytic step 2 spliceosome GO:0071007 ISS
    precatalytic spliceosome GO:0071011 IBA
    catalytic step 2 spliceosome GO:0071013 IBA
    catalytic step 2 spliceosome GO:0071013 ISO
    spliceosomal tri-snRNP complex GO:0097526 IBA
 Experiment description of studies that identified Snrpd3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 263
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Snrpd3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sf3a3 75062
Co-fractionation Mus musculus
2 Stxbp3 20912
Co-fractionation Mus musculus
3 Banf1 23825
Co-fractionation Mus musculus
4 Stxbp2 20911
Co-fractionation Mus musculus
5 Snrpd1 20641
Co-fractionation Mus musculus
6 Srrm1 51796
Co-fractionation Mus musculus
7 Strap 20901
Co-fractionation Mus musculus
8 Plrg1  
Co-fractionation Mus musculus
9 Alyref  
Co-fractionation Mus musculus
10 Snrnp200 320632
Co-fractionation Mus musculus
11 Myoz2  
Cross-Linking-MS (XL-MS) Mus musculus
12 Snrpd2 107686
Co-fractionation Mus musculus
13 Mecp2  
Affinity Capture-MS Mus musculus
14 Sf3b3 101943
Co-fractionation Mus musculus
15 Mettl21e  
Affinity Capture-MS Mus musculus
16 Hnrnph1 59013
Co-fractionation Mus musculus
17 Ybx1 22608
Co-fractionation Mus musculus
18 Pabpc1 18458
Co-fractionation Mus musculus
19 Srrm2  
Co-fractionation Mus musculus
20 Puf60 67959
Co-fractionation Mus musculus
21 Syncrip 56403
Co-fractionation Mus musculus
22 Ppwd1  
Co-fractionation Mus musculus
23 Sfpq 71514
Co-fractionation Mus musculus
24 Dhx15 13204
Co-fractionation Mus musculus
25 Fancd2  
Affinity Capture-MS Mus musculus
26 Hnrnpk 15387
Co-fractionation Mus musculus
27 Ppil3  
Co-fractionation Mus musculus
28 Eed  
Affinity Capture-MS Mus musculus
29 U2af2 22185
Co-fractionation Mus musculus
30 Skiv2l2  
Co-fractionation Mus musculus
31 Prpf31  
Co-fractionation Mus musculus
32 Atg12  
Positive Genetic Mus musculus
33 Lgals1 16852
Co-fractionation Mus musculus
34 Eftud2 20624
Co-fractionation Mus musculus
35 Hnrnpa2b1 53379
Co-fractionation Mus musculus
View the network image/svg+xml



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