Gene description for PFKFB2
Gene name 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
Gene symbol PFKFB2
Other names/aliases PFK-2/FBPase-2
Species Homo sapiens
 Database cross references - PFKFB2
ExoCarta ExoCarta_5208
Vesiclepedia VP_5208
Entrez Gene 5208
HGNC 8873
MIM 171835
UniProt O60825  
 PFKFB2 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for PFKFB2
Molecular Function
    6-phosphofructo-2-kinase activity GO:0003873 IBA
    6-phosphofructo-2-kinase activity GO:0003873 IDA
    fructose-2,6-bisphosphate 2-phosphatase activity GO:0004331 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    protein kinase binding GO:0019901 IPI
Biological Process
    fructose metabolic process GO:0006000 IEA
    fructose 2,6-bisphosphate metabolic process GO:0006003 IBA
    glucose catabolic process GO:0006007 IEA
    lactate metabolic process GO:0006089 IEA
    glycolytic process GO:0006096 IDA
    response to glucose GO:0009749 IEA
    positive regulation of insulin secretion GO:0032024 IEA
    carbohydrate phosphorylation GO:0046835 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified PFKFB2 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PFKFB2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
3 PFKFB4 5210
Affinity Capture-MS Homo sapiens
4 PFKFB3 5209
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 POLDIP3 84271
Affinity Capture-MS Homo sapiens
6 FAM83B  
Affinity Capture-MS Homo sapiens
7 COPS2 9318
Co-fractionation Homo sapiens
8 DOCK8 81704
Affinity Capture-MS Homo sapiens
9 ESR1  
Affinity Capture-MS Homo sapiens
10 CPSF7 79869
Affinity Capture-MS Homo sapiens
11 SMURF1 57154
Affinity Capture-MS Homo sapiens
12 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 SH2D3A  
Affinity Capture-MS Homo sapiens
14 YWHAE 7531
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 SPSB4  
Affinity Capture-MS Homo sapiens
16 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 GCK  
Two-hybrid Homo sapiens
18 PDE4D  
Affinity Capture-MS Homo sapiens
19 UBC 7316
Affinity Capture-MS Homo sapiens
20 RBM15 64783
Affinity Capture-MS Homo sapiens
21 WHSC1 7468
Affinity Capture-MS Homo sapiens
22 SNX27 81609
Affinity Capture-MS Homo sapiens
23 ZC3H11A  
Affinity Capture-MS Homo sapiens
24 PSMD14 10213
Affinity Capture-MS Homo sapiens
25 SCYL2 55681
Affinity Capture-MS Homo sapiens
26 UFL1 23376
Affinity Capture-MS Homo sapiens
27 KIAA1429 25962
Affinity Capture-MS Homo sapiens
28 HIST1H3E 8353
Affinity Capture-MS Homo sapiens
29 CUL9  
Affinity Capture-MS Homo sapiens
30 ZCCHC17  
Affinity Capture-MS Homo sapiens
31 CBY1  
Affinity Capture-MS Homo sapiens
32 RPA3 6119
Proximity Label-MS Homo sapiens
33 TRIM21 6737
Affinity Capture-MS Homo sapiens
34 MOB2 81532
Affinity Capture-MS Homo sapiens
35 RAB3IP  
Affinity Capture-MS Homo sapiens
36 PGAP2  
Affinity Capture-MS Homo sapiens
37 PARVB 29780
Co-fractionation Homo sapiens
38 YWHAQ 10971
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
39 RPA4  
Proximity Label-MS Homo sapiens
40 CUL7 9820
Affinity Capture-MS Homo sapiens
41 SRRM2 23524
Affinity Capture-MS Homo sapiens
42 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 SARNP 84324
Affinity Capture-MS Homo sapiens
44 PRMT1 3276
Affinity Capture-MS Homo sapiens
45 CYLD  
Affinity Capture-MS Homo sapiens
46 RMDN3 55177
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here