Gene description for NDUFB3
Gene name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 3, 12kDa
Gene symbol NDUFB3
Other names/aliases B12
CI-B12
Species Homo sapiens
 Database cross references - NDUFB3
ExoCarta ExoCarta_4709
Vesiclepedia VP_4709
Entrez Gene 4709
HGNC 7698
MIM 603839
UniProt O43676  
 NDUFB3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for NDUFB3
Molecular Function
    NADH dehydrogenase (ubiquinone) activity GO:0008137 NAS
Biological Process
    mitochondrial electron transport, NADH to ubiquinone GO:0006120 NAS
    aerobic respiration GO:0009060 NAS
    mitochondrial respiratory chain complex I assembly GO:0032981 IBA
    mitochondrial respiratory chain complex I assembly GO:0032981 IMP
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 NAS
    proton transmembrane transport GO:1902600 IEA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial inner membrane GO:0005743 TAS
    respiratory chain complex I GO:0045271 IBA
    respiratory chain complex I GO:0045271 IDA
    respiratory chain complex I GO:0045271 NAS
 Experiment description of studies that identified NDUFB3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NDUFB3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CSGALNACT2 55454
Affinity Capture-MS Homo sapiens
2 NDUFS1 4719
Affinity Capture-MS Homo sapiens
3 NDUFB5 4711
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 NDUFA12 55967
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 DPEP1 1800
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 PRRT2  
Affinity Capture-MS Homo sapiens
7 NDUFA4 4697
Affinity Capture-MS Homo sapiens
8 SLC22A9  
Affinity Capture-MS Homo sapiens
9 APP 351
Reconstituted Complex Homo sapiens
10 NDUFB9 4715
Affinity Capture-MS Homo sapiens
11 TCTEX1D2  
Affinity Capture-MS Homo sapiens
12 HEATR3 55027
Affinity Capture-MS Homo sapiens
13 NDUFS3 4722
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 C11orf87  
Affinity Capture-MS Homo sapiens
15 NDUFB8 4714
Affinity Capture-MS Homo sapiens
16 GPR34  
Affinity Capture-MS Homo sapiens
17 NDUFA7 4701
Affinity Capture-MS Homo sapiens
18 OPRM1 4988
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 NDUFA5 4698
Affinity Capture-MS Homo sapiens
20 YIPF3 25844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 NDUFA3  
Affinity Capture-MS Homo sapiens
22 NDUFV1 4723
Affinity Capture-MS Homo sapiens
23 NDUFV3 4731
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 NDUFA11 126328
Affinity Capture-MS Homo sapiens
25 NDUFA6  
Affinity Capture-MS Homo sapiens
26 NDUFS5 4725
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 GPR17 2840
Affinity Capture-MS Homo sapiens
28 NDUFB1 4707
Affinity Capture-MS Homo sapiens
29 ENPP6 133121
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 EXTL3 2137
Affinity Capture-MS Homo sapiens
31 NDUFS4 4724
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
32 NDUFB7 4713
Affinity Capture-MS Homo sapiens
33 ND2 4536
Affinity Capture-MS Homo sapiens
34 EMP3 2014
Affinity Capture-MS Homo sapiens
35 NDUFA8 4702
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 KDSR 2531
Affinity Capture-MS Homo sapiens
37 CYB5A 1528
Co-fractionation Homo sapiens
38 MYH9 4627
Affinity Capture-MS Homo sapiens
39 ND1  
Affinity Capture-MS Homo sapiens
40 IPPK  
Affinity Capture-MS Homo sapiens
41 BTNL3  
Affinity Capture-MS Homo sapiens
42 DNAJC30  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 COX4I1 1327
Co-fractionation Homo sapiens
44 GJA8  
Affinity Capture-MS Homo sapiens
45 C15orf48  
Affinity Capture-MS Homo sapiens
46 SV2A 9900
Affinity Capture-MS Homo sapiens
47 NDUFAB1 4706
Affinity Capture-MS Homo sapiens
48 VAPB 9217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 NDUFB10 4716
Affinity Capture-MS Homo sapiens
50 NDUFA13 51079
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 NDUFA9 4704
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 ND5 4540
Affinity Capture-MS Homo sapiens
53 GALNT6 11226
Affinity Capture-MS Homo sapiens
54 CRELD1 78987
Affinity Capture-MS Homo sapiens
55 MGARP  
Affinity Capture-MS Homo sapiens
56 GPR12  
Affinity Capture-MS Homo sapiens
57 BCAP31 10134
Co-fractionation Homo sapiens
58 AQP3  
Affinity Capture-MS Homo sapiens
59 OPRL1 4987
Affinity Capture-MS Homo sapiens
60 LPAR1 1902
Affinity Capture-MS Homo sapiens
61 CASD1  
Affinity Capture-MS Homo sapiens
62 COX6C 1345
Co-fractionation Homo sapiens
63 CYP2J2  
Affinity Capture-MS Homo sapiens
64 NDUFB6 4712
Affinity Capture-MS Homo sapiens
65 PRSS8 5652
Affinity Capture-MS Homo sapiens
66 P2RY10  
Affinity Capture-MS Homo sapiens
67 NDUFA10 4705
Affinity Capture-MS Homo sapiens
68 NDUFV2 4729
Affinity Capture-MS Homo sapiens
69 NDUFC2 4718
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 ADGRE5 976
Affinity Capture-MS Homo sapiens
71 NDUFS2 4720
Affinity Capture-MS Homo sapiens
72 FUT1  
Affinity Capture-MS Homo sapiens
73 PHGDH 26227
Affinity Capture-MS Homo sapiens
74 SAYSD1  
Affinity Capture-MS Homo sapiens
75 NDUFA1 4694
Affinity Capture-MS Homo sapiens
76 FNDC4 64838
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
78 TACR1  
Affinity Capture-MS Homo sapiens
79 SIDT2 51092
Affinity Capture-MS Homo sapiens
80 C3AR1  
Affinity Capture-MS Homo sapiens
81 NDUFS7 374291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 WIF1 11197
Affinity Capture-MS Homo sapiens
83 NDUFS8 4728
Affinity Capture-MS Homo sapiens
84 NDUFA2 4695
Affinity Capture-MS Homo sapiens
85 COX7A2 1347
Cross-Linking-MS (XL-MS) Homo sapiens
86 NDUFAF4 29078
Affinity Capture-MS Homo sapiens
87 ATP5C1 509
Two-hybrid Homo sapiens
88 NDUFB11 54539
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 CCR6  
Affinity Capture-MS Homo sapiens
90 ND4 4538
Affinity Capture-MS Homo sapiens
91 NDUFS6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 NDUFB4 4710
Affinity Capture-MS Homo sapiens
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