Gene description for PPCS
Gene name phosphopantothenoylcysteine synthetase
Gene symbol PPCS
Other names/aliases -
Species Homo sapiens
 Database cross references - PPCS
ExoCarta ExoCarta_79717
Vesiclepedia VP_79717
Entrez Gene 79717
HGNC 25686
MIM 609853
UniProt Q9HAB8  
 PPCS identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for PPCS
Molecular Function
    phosphopantothenate--cysteine ligase activity GO:0004632 IBA
    phosphopantothenate--cysteine ligase activity GO:0004632 IDA
    phosphopantothenate--cysteine ligase activity GO:0004632 IMP
    ATP binding GO:0005524 IEA
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IMP
Biological Process
    heart process GO:0003015 IMP
    acetyl-CoA biosynthetic process GO:0006085 IMP
    coenzyme A biosynthetic process GO:0015937 IBA
    coenzyme A biosynthetic process GO:0015937 IDA
    coenzyme A biosynthetic process GO:0015937 IEA
    coenzyme A biosynthetic process GO:0015937 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified PPCS in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PPCS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RIT1 6016
Negative Genetic Homo sapiens
2 GXYLT2  
Affinity Capture-MS Homo sapiens
3 PNPLA4  
Affinity Capture-MS Homo sapiens
4 SPRED2 200734
Affinity Capture-MS Homo sapiens
5 RPA2 6118
Proximity Label-MS Homo sapiens
6 CCR1  
Affinity Capture-MS Homo sapiens
7 IGHA2 3494
Affinity Capture-MS Homo sapiens
8 PDZRN3 23024
Affinity Capture-MS Homo sapiens
9 RPE  
Co-fractionation Homo sapiens
10 IDE 3416
Co-fractionation Homo sapiens
11 PPCS 79717
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
12 HP 3240
Affinity Capture-MS Homo sapiens
13 LACRT 90070
Affinity Capture-MS Homo sapiens
14 RPA3 6119
Proximity Label-MS Homo sapiens
15 BST1 683
Affinity Capture-MS Homo sapiens
16 BPIFB1 92747
Affinity Capture-MS Homo sapiens
17 TRMT44  
Affinity Capture-MS Homo sapiens
18 SCGB2A1 4246
Affinity Capture-MS Homo sapiens
19 SERBP1 26135
Affinity Capture-MS Homo sapiens
20 KRAS 3845
Negative Genetic Homo sapiens
21 C5orf46 389336
Affinity Capture-MS Homo sapiens
22 LTF 4057
Affinity Capture-MS Homo sapiens
23 MPI 4351
Affinity Capture-MS Homo sapiens
24 LMNA 4000
Proximity Label-MS Homo sapiens
25 DMBT1 1755
Affinity Capture-MS Homo sapiens
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