Gene description for VPS41
Gene name vacuolar protein sorting 41 homolog (S. cerevisiae)
Gene symbol VPS41
Other names/aliases HVPS41
HVSP41
hVps41p
Species Homo sapiens
 Database cross references - VPS41
ExoCarta ExoCarta_27072
Vesiclepedia VP_27072
Entrez Gene 27072
HGNC 12713
MIM 605485
UniProt P49754  
 VPS41 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for VPS41
Molecular Function
    protein binding GO:0005515 IPI
    microtubule binding GO:0008017 IEA
    identical protein binding GO:0042802 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    protein targeting to vacuole GO:0006623 IBA
    endosome to lysosome transport GO:0008333 IMP
    cellular response to starvation GO:0009267 IBA
    vesicle-mediated transport GO:0016192 IMP
    macroautophagy GO:0016236 IBA
    endosomal vesicle fusion GO:0034058 IBA
    endosomal vesicle fusion GO:0034058 IMP
    endosomal vesicle fusion GO:0034058 NAS
    regulation of SNARE complex assembly GO:0035542 NAS
    Golgi vesicle transport GO:0048193 IMP
    late endosome to lysosome transport GO:1902774 IMP
Subcellular Localization
    lysosome GO:0005764 IDA
    lysosomal membrane GO:0005765 HDA
    lysosomal membrane GO:0005765 IDA
    late endosome GO:0005770 IBA
    late endosome GO:0005770 IDA
    Golgi apparatus GO:0005794 IEA
    Golgi-associated vesicle GO:0005798 IMP
    cytosol GO:0005829 IDA
    cytosol GO:0005829 IEA
    endosome membrane GO:0010008 IDA
    endosome membrane GO:0010008 TAS
    microtubule cytoskeleton GO:0015630 IEA
    membrane GO:0016020 IDA
    AP-3 adaptor complex GO:0030123 IDA
    clathrin-coated vesicle GO:0030136 IDA
    HOPS complex GO:0030897 IBA
    HOPS complex GO:0030897 IDA
    HOPS complex GO:0030897 IMP
    HOPS complex GO:0030897 NAS
    early endosome membrane GO:0031901 IEA
    late endosome membrane GO:0031902 IDA
    clathrin complex GO:0071439 IDA
    lysosomal HOPS complex GO:1902501 IDA
 Experiment description of studies that identified VPS41 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for VPS41
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UTP20 27340
Co-fractionation Homo sapiens
2 VPS16 64601
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 SNX27 81609
Affinity Capture-MS Homo sapiens
4 AKTIP  
Affinity Capture-Western Homo sapiens
5 HOOK1  
Affinity Capture-Western Homo sapiens
6 RILP 83547
Reconstituted Complex Homo sapiens
7 VIPAS39 63894
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
8 PLBD1 79887
Affinity Capture-MS Homo sapiens
9 TGFBRAP1 9392
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 MON1B 22879
Reconstituted Complex Homo sapiens
11 TRIM35 23087
Affinity Capture-MS Homo sapiens
12 FAM96A  
Affinity Capture-MS Homo sapiens
13 RBM25 58517
Affinity Capture-MS Homo sapiens
14 SULT1C4  
Affinity Capture-MS Homo sapiens
15 NABP2  
Affinity Capture-MS Homo sapiens
16 PRPF40A 55660
Affinity Capture-MS Homo sapiens
17 HOOK3 84376
Affinity Capture-Western Homo sapiens
18 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
19 HOOK2  
Affinity Capture-Western Homo sapiens
20 CLTB 1212
Affinity Capture-Western Homo sapiens
21 APP 351
Reconstituted Complex Homo sapiens
22 TRMU  
Affinity Capture-MS Homo sapiens
23 Max  
Affinity Capture-MS Mus musculus
24 VPS33A 65082
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 STX17 55014
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
26 XRCC4  
Co-fractionation Homo sapiens
27 TECPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 C12orf49  
Negative Genetic Homo sapiens
29 CTDSP2 10106
Affinity Capture-MS Homo sapiens
30 C12orf74  
Affinity Capture-MS Homo sapiens
31 PRC1 9055
Affinity Capture-MS Homo sapiens
32 RAB7A 7879
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
33 D2HGDH 728294
Affinity Capture-MS Homo sapiens
34 RFXANK  
Affinity Capture-MS Homo sapiens
35 UBE2U  
Two-hybrid Homo sapiens
36 CT55  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 ZUFSP 221302
Affinity Capture-MS Homo sapiens
38 UBXN6 80700
Affinity Capture-MS Homo sapiens
39 BICD2 23299
Affinity Capture-MS Homo sapiens
40 GGTLC1 92086
Affinity Capture-MS Homo sapiens
41 ZNF512B  
Two-hybrid Homo sapiens
42 VPS39 23339
Affinity Capture-Western Homo sapiens
43 AP3D1 8943
Affinity Capture-Western Homo sapiens
44 VPS33B 26276
Affinity Capture-Western Homo sapiens
45 INF2 64423
Affinity Capture-MS Homo sapiens
46 VPS18 57617
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
47 TCEAL4  
Affinity Capture-MS Homo sapiens
48 KDM5C  
Affinity Capture-MS Homo sapiens
49 METTL21B  
Affinity Capture-MS Homo sapiens
50 VPS11 55823
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 PTAR1 375743
Synthetic Lethality Homo sapiens
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