Gene description for DUSP6
Gene name dual specificity phosphatase 6
Gene symbol DUSP6
Other names/aliases HH19
MKP3
PYST1
Species Homo sapiens
 Database cross references - DUSP6
ExoCarta ExoCarta_1848
Entrez Gene 1848
HGNC 3072
MIM 602748
UniProt Q16828  
 DUSP6 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for DUSP6
Molecular Function
    protein tyrosine phosphatase activity GO:0004725 IEA
    MAP kinase tyrosine/serine/threonine phosphatase activity GO:0017017 IDA
Biological Process
    cell differentiation GO:0030154 IEA
    toll-like receptor TLR6:TLR2 signaling pathway GO:0038124 TAS
    response to nitrosative stress GO:0051409 IEP
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 TAS
    toll-like receptor 10 signaling pathway GO:0034166 TAS
    dorsal/ventral pattern formation GO:0009953 IBA
    peptidyl-tyrosine dephosphorylation GO:0035335 IDA
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    positive regulation of apoptotic process GO:0043065 IDA
    regulation of fibroblast growth factor receptor signaling pathway GO:0040036 IBA
    negative regulation of ERK1 and ERK2 cascade GO:0070373 IMP
    inactivation of MAPK activity GO:0000188 IDA
    toll-like receptor signaling pathway GO:0002224 TAS
    regulation of heart growth GO:0060420 IBA
    toll-like receptor 5 signaling pathway GO:0034146 TAS
    stress-activated MAPK cascade GO:0051403 TAS
    regulation of endodermal cell fate specification GO:0042663 IBA
    innate immune response GO:0045087 TAS
    toll-like receptor 3 signaling pathway GO:0034138 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    toll-like receptor 2 signaling pathway GO:0034134 TAS
    response to drug GO:0042493 IEA
    response to organic cyclic compound GO:0014070 IEA
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    MyD88-independent toll-like receptor signaling pathway GO:0002756 TAS
    toll-like receptor 9 signaling pathway GO:0034162 TAS
    toll-like receptor TLR1:TLR2 signaling pathway GO:0038123 TAS
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 IEA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified DUSP6 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for DUSP6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAPK1 5594
Invitro Homo sapiens
2 TEX11  
Two-hybrid Homo sapiens
3 MAPK14 1432
Affinity Capture-Western Homo sapiens
4 MAPK3 5595
Invitro Homo sapiens
5 MDFI  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which DUSP6 is involved
PathwayEvidenceSource
ERKs are inactivated TAS Reactome
ERKs are inactivated TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here