Gene description for LEMD2
Gene name LEM domain containing 2
Gene symbol LEMD2
Other names/aliases NET25
dJ482C21.1
Species Homo sapiens
 Database cross references - LEMD2
ExoCarta ExoCarta_221496
Vesiclepedia VP_221496
Entrez Gene 221496
HGNC 21244
MIM 616312
UniProt Q8NC56  
 LEMD2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for LEMD2
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    nuclear envelope organization GO:0006998 IMP
    neurogenesis GO:0022008 IEA
    skeletal muscle cell differentiation GO:0035914 IGI
    negative regulation of MAPK cascade GO:0043409 IEA
    negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051898 IEA
    heart formation GO:0060914 IEA
    protein localization to chromatin GO:0071168 IMP
    nuclear membrane organization GO:0071763 IBA
Subcellular Localization
    chromatin GO:0000785 IDA
    nuclear envelope GO:0005635 IDA
    nuclear envelope GO:0005635 TAS
    nuclear inner membrane GO:0005637 IBA
    nuclear inner membrane GO:0005637 IDA
    nuclear inner membrane GO:0005637 TAS
    endoplasmic reticulum GO:0005783 IDA
    spindle GO:0005819 IEA
    membrane GO:0016020 HDA
    membrane GO:0016020 TAS
    nuclear membrane GO:0031965 IDA
    nuclear periphery GO:0034399 IBA
 Experiment description of studies that identified LEMD2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for LEMD2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLC39A12  
Affinity Capture-MS Homo sapiens
2 ANLN 54443
Affinity Capture-MS Homo sapiens
3 SGCA  
Affinity Capture-MS Homo sapiens
4 HTR3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 TMEM169  
Affinity Capture-MS Homo sapiens
6 Tmed2 56334
Affinity Capture-MS Mus musculus
7 BACE2 25825
Affinity Capture-MS Homo sapiens
8 TSPAN5 10098
Affinity Capture-MS Homo sapiens
9 CSNK2B 1460
Affinity Capture-MS Homo sapiens
10 CHRNB3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 HMGN4  
Affinity Capture-MS Homo sapiens
12 CHRNA4  
Affinity Capture-MS Homo sapiens
13 TTYH1  
Affinity Capture-MS Homo sapiens
14 TSPAN15 23555
Affinity Capture-MS Homo sapiens
15 KIF14 9928
Affinity Capture-MS Homo sapiens
16 SLC39A4 55630
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 VTN 7448
Affinity Capture-MS Homo sapiens
18 SOX2  
Affinity Capture-MS Homo sapiens
19 MME 4311
Affinity Capture-MS Homo sapiens
20 NF2 4771
Affinity Capture-MS Homo sapiens
21 KCNK1  
Affinity Capture-MS Homo sapiens
22 KCNC4  
Affinity Capture-MS Homo sapiens
23 SLC39A8 64116
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 NOP56 10528
Affinity Capture-MS Homo sapiens
25 CRELD1 78987
Affinity Capture-MS Homo sapiens
26 FOXA1  
Affinity Capture-MS Homo sapiens
27 SYNE4  
Affinity Capture-MS Homo sapiens
28 RPL10 6134
Affinity Capture-MS Homo sapiens
29 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
30 NHLRC2 374354
Affinity Capture-MS Homo sapiens
31 IFI6  
Affinity Capture-MS Homo sapiens
32 B4GAT1 11041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 ADCY8  
Affinity Capture-MS Homo sapiens
34 PARK2  
Affinity Capture-MS Homo sapiens
35 DLK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 Ktn1  
Affinity Capture-MS Mus musculus
37 LGR5 8549
Affinity Capture-MS Homo sapiens
38 TMEM74  
Affinity Capture-MS Homo sapiens
39 ATP2A3 489
Affinity Capture-MS Homo sapiens
40 ZDHHC12  
Affinity Capture-MS Homo sapiens
41 ANO6 196527
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 SAAL1 113174
Affinity Capture-MS Homo sapiens
43 PDE3A  
Affinity Capture-MS Homo sapiens
44 PLD3 23646
Affinity Capture-MS Homo sapiens
45 CHRNA9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 CHRNB4  
Affinity Capture-MS Homo sapiens
47 PBXIP1 57326
Affinity Capture-MS Homo sapiens
48 BTF3 689
Affinity Capture-MS Homo sapiens
49 KCNA10  
Affinity Capture-MS Homo sapiens
50 GDPD5 81544
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 OGT 8473
Reconstituted Complex Homo sapiens
52 AIM2  
Affinity Capture-MS Homo sapiens
53 SORT1 6272
Affinity Capture-MS Homo sapiens
54 ATP2B2 491
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 NUP35 129401
Proximity Label-MS Homo sapiens
56 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
57 ASPH 444
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 PRC1 9055
Affinity Capture-MS Homo sapiens
59 SGSM3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
61 CMKLR1 1240
Affinity Capture-MS Homo sapiens
62 UPK1A 11045
Affinity Capture-MS Homo sapiens
63 KCNA2  
Affinity Capture-MS Homo sapiens
64 CHRNB1  
Affinity Capture-MS Homo sapiens
65 LMNA 4000
Proximity Label-MS Homo sapiens
66 RPA3 6119
Proximity Label-MS Homo sapiens
67 UQCR11  
Affinity Capture-MS Homo sapiens
68 HAUS7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 ADGRE5 976
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 C3orf52  
Affinity Capture-MS Homo sapiens
71 CHRNA3 1136
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 KCNA5  
Affinity Capture-MS Homo sapiens
73 DGCR2 9993
Affinity Capture-MS Homo sapiens
74 SLC7A1 6541
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 ATP2A1 487
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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