Experiment description of studies that identified TMEM200A in sEVs

1 Experiment ID 207 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DKO-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

2 Experiment ID 208 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Dks-8 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 209 MISEV standards ✘ Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DLD-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 1203 MISEV standards ✔ EM Biophysical techniques ✔ SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 34887515     Organism Homo sapiens Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." Journal name Nat Cell Biol Publication year 2021 Sample Colorectal cancer cells Sample name DiFi Isolation/purification methods Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Western blotting Mass spectrometry RNA sequencing

5 Experiment ID 488 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

Protein-protein interactions for TMEM200A

Protein Interactor ExoCarta ID Identification method PubMed Species 1 STX7 8417 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 2 AXL 558 Affinity Capture-MS Homo sapiens 3 PPP3CA 5530 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 4 MYADM 91663 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 5 PPP3R1 5534 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 6 PCDHGA6 56109 Affinity Capture-MS Homo sapiens 7 STX10 8677 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 8 ERBB2 2064 Affinity Capture-MS Homo sapiens 9 TGFBR2 7048 Affinity Capture-MS Homo sapiens 10 PLEKHH3   Affinity Capture-MS Homo sapiens 11 RHBDF2 79651 Affinity Capture-MS Homo sapiens 12 TP53 7157 Synthetic Growth Defect Homo sapiens 13 GOSR1 9527 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 14 BMPR1A 657 Affinity Capture-MS Homo sapiens 15 NR3C1 2908 Proximity Label-MS Homo sapiens 16 TMEM9B 56674 Affinity Capture-MS Homo sapiens 17 EMD 2010 Two-hybrid Homo sapiens 18 PPP3R2   Affinity Capture-MS Homo sapiens 19 IFNGR1 3459 Affinity Capture-MS Homo sapiens 20 FHOD1 29109 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 21 KCNE3   Affinity Capture-MS Homo sapiens 22 ACVR1B 91 Affinity Capture-MS Homo sapiens 23 KLHDC8A   Affinity Capture-MS Homo sapiens 24 INSRR 3645 Affinity Capture-MS Homo sapiens 25 DDR1 780 Affinity Capture-MS Homo sapiens 26 PPP3CB   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 27 BSND   Affinity Capture-MS Homo sapiens 28 STX6 10228 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 29 UNC119 9094 Two-hybrid Homo sapiens 30 ERC1 23085 Cross-Linking-MS (XL-MS) Homo sapiens 31 OSMR 9180 Affinity Capture-MS Homo sapiens 32 SLC31A1 1317 Affinity Capture-MS Homo sapiens 33 PIGH   Affinity Capture-MS Homo sapiens 34 C9orf91 203197 Affinity Capture-MS Homo sapiens 35 CAMK2G 818 Affinity Capture-MS Homo sapiens 36 BCL2L10   Two-hybrid Homo sapiens 37 PPP3CC 5533 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens View the network

Pathways in which TMEM200A is involved

No pathways found