Gene description for ITCH
Gene name itchy E3 ubiquitin protein ligase
Gene symbol ITCH
Other names/aliases ADMFD
AIF4
AIP4
NAPP1
dJ468O1.1
Species Homo sapiens
 Database cross references - ITCH
ExoCarta ExoCarta_83737
Vesiclepedia VP_83737
Entrez Gene 83737
HGNC 13890
MIM 606409
UniProt Q96J02  
 ITCH identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Prostate cancer cells 25844599    
Thymus 23844026    
 Gene ontology annotations for ITCH
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 EXP
    protein binding GO:0005515 IPI
    ligase activity GO:0016874 IEA
    ubiquitin-like protein transferase activity GO:0019787 TAS
    ubiquitin-ubiquitin ligase activity GO:0034450 IDA
    ribonucleoprotein complex binding GO:0043021 IPI
    ubiquitin-like protein ligase binding GO:0044389 IPI
    CXCR chemokine receptor binding GO:0045236 IPI
    ubiquitin protein ligase activity GO:0061630 IBA
    ubiquitin protein ligase activity GO:0061630 IDA
    ubiquitin protein ligase activity GO:0061630 IMP
    ubiquitin protein ligase activity GO:0061630 NAS
    ubiquitin protein ligase activity GO:0061630 TAS
    arrestin family protein binding GO:1990763 IPI
Biological Process
    regulation of cell growth GO:0001558 NAS
    positive regulation of T cell anergy GO:0002669 IEA
    T cell anergy GO:0002870 IEA
    ubiquitin-dependent protein catabolic process GO:0006511 IDA
    ubiquitin-dependent protein catabolic process GO:0006511 IMP
    ubiquitin-dependent protein catabolic process GO:0006511 NAS
    protein monoubiquitination GO:0006513 IDA
    apoptotic process GO:0006915 IEA
    inflammatory response GO:0006954 NAS
    protein ubiquitination GO:0016567 IDA
    protein ubiquitination GO:0016567 IMP
    receptor internalization GO:0031623 IMP
    negative regulation of NF-kappaB transcription factor activity GO:0032088 ISS
    negative regulation of type I interferon production GO:0032480 TAS
    protein K29-linked ubiquitination GO:0035519 IDA
    CD4-positive, alpha-beta T cell proliferation GO:0035739 IEA
    nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway GO:0035872 TAS
    negative regulation of cytoplasmic pattern recognition receptor signaling pathway GO:0039532 IDA
    negative regulation of apoptotic process GO:0043066 IMP
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IDA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IMP
    innate immune response GO:0045087 IEA
    positive regulation of protein catabolic process GO:0045732 IEA
    negative regulation of JNK cascade GO:0046329 ISS
    symbiont entry into host cell GO:0046718 TAS
    negative regulation of defense response to virus GO:0050687 IMP
    defense response to virus GO:0051607 IEA
    protein autoubiquitination GO:0051865 IMP
    regulation of necroptotic process GO:0060544 TAS
    protein K63-linked ubiquitination GO:0070534 IDA
    protein K48-linked ubiquitination GO:0070936 IDA
    regulation of protein deubiquitination GO:0090085 ISS
    protein branched polyubiquitination GO:0141198 IDA
    regulation of hematopoietic stem cell differentiation GO:1902036 TAS
    negative regulation of CD4-positive, alpha-beta T cell proliferation GO:2000562 IEA
    positive regulation of receptor catabolic process GO:2000646 IMP
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 IC
    early endosome GO:0005769 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IDA
    cell cortex GO:0005938 IEA
    membrane GO:0016020 HDA
    cytoplasmic vesicle GO:0031410 IDA
    early endosome membrane GO:0031901 IEA
    protein-containing complex GO:0032991 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified ITCH in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 498
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 207
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 208
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 1203
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
13
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
16
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
17
Experiment ID 1275
MISEV standards
Biophysical techniques
✔ Enriched markers
✔ Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 1280
MISEV standards
Biophysical techniques
✔ Enriched markers
✔ Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
19
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
20
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
21
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
22
Experiment ID 255
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
23
Experiment ID 256
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
24
Experiment ID 260
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
25
Experiment ID 488
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
26
Experiment ID 224
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
27
Experiment ID 211
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
28
Experiment ID 212
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
29
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
30
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
31
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
32
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
33
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
34
Experiment ID 274
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
35
Experiment ID 217
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ITCH
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LRP3  
Affinity Capture-MS Homo sapiens
2 POLR2B 5431
Reconstituted Complex Homo sapiens
3 GRAP2 9402
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 CDC34 997
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
5 SLC1A5 6510
Affinity Capture-MS Homo sapiens
6 OCLN 100506658
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Proximity Label-MS Homo sapiens
7 SCNN1A  
Far Western Homo sapiens
8 UBE2K 3093
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
9 TNFAIP3 7128
Affinity Capture-Western Homo sapiens
10 C11orf52 91894
Proximity Label-MS Homo sapiens
11 USP13  
Affinity Capture-MS Homo sapiens
12 UBE2M 9040
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
13 RPA2 6118
Co-fractionation Homo sapiens
14 USP5 8078
Affinity Capture-MS Homo sapiens
15 ADRB2  
Affinity Capture-Western Homo sapiens
16 UBA1 7317
Affinity Capture-MS Homo sapiens
17 UBE2C 11065
Reconstituted Complex Homo sapiens
18 SMARCE1 6605
Reconstituted Complex Homo sapiens
19 USP9X 8239
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
20 TAB1 10454
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
21 UBC 7316
Biochemical Activity Homo sapiens
Protein-peptide Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
22 CSNK2A1 1457
Biochemical Activity Homo sapiens
23 KPNA2 3838
Affinity Capture-MS Homo sapiens
24 DVL3 1857
Affinity Capture-Western Homo sapiens
25 RIPK1 8737
Affinity Capture-Western Homo sapiens
26 ATN1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
27 KPNB1 3837
Reconstituted Complex Homo sapiens
28 SOX2  
Affinity Capture-MS Homo sapiens
29 MARCH8  
Affinity Capture-MS Homo sapiens
30 URI1  
Reconstituted Complex Homo sapiens
31 TGFBR1 7046
Affinity Capture-Western Homo sapiens
32 CAPZB 832
Affinity Capture-MS Homo sapiens
33 LAMTOR1 55004
Proximity Label-MS Homo sapiens
34 WDR48 57599
Affinity Capture-Western Homo sapiens
35 JAG1 182
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
36 UBE2E2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
37 UBE2O 63893
Reconstituted Complex Homo sapiens
38 UBE2J2 118424
Reconstituted Complex Homo sapiens
39 Mlkl  
Reconstituted Complex Mus musculus
Biochemical Activity Mus musculus
40 DAZAP1 26528
Reconstituted Complex Homo sapiens
41 COPB2 9276
Affinity Capture-MS Homo sapiens
42 IMPDH2 3615
Co-fractionation Homo sapiens
43 HGS 9146
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
44 CPSF7 79869
Reconstituted Complex Homo sapiens
45 POU5F1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
46 TNFRSF19  
Affinity Capture-MS Homo sapiens
47 DTX1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
48 BAX 581
Affinity Capture-Western Homo sapiens
49 ARID1A 8289
Reconstituted Complex Homo sapiens
50 GEMIN8 54960
Affinity Capture-Western Homo sapiens
51 RASSF5 83593
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
52 UBE2Q1 55585
Reconstituted Complex Homo sapiens
53 USP27X  
Affinity Capture-Western Homo sapiens
54 POLR2C 5432
Reconstituted Complex Homo sapiens
55 SFN 2810
Affinity Capture-MS Homo sapiens
56 LDLRAD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 MAVS 57506
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
58 WBP2 23558
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
59 CFLAR  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
60 UBE2E3 10477
Reconstituted Complex Homo sapiens
61 DVL2 1856
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
62 RNF11 26994
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
63 DDX58 23586
Affinity Capture-RNA Homo sapiens
64 EWSR1 2130
Reconstituted Complex Homo sapiens
65 TMEM255A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 WWP1 11059
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 POLR2E 5434
Reconstituted Complex Homo sapiens
68 AGO2 27161
Protein-RNA Homo sapiens
69 NDFIP1 80762
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
70 NEDD9 4739
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
71 TOLLIP 54472
Affinity Capture-MS Homo sapiens
72 USP12 219333
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
73 ARRDC1 92714
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
74 UBAP2 55833
Reconstituted Complex Homo sapiens
75 WBP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 LAPTM5 7805
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
77 UBE2D4  
Reconstituted Complex Homo sapiens
78 ERBB3 2065
Affinity Capture-Western Homo sapiens
79 LMP2A  
Reconstituted Complex
Affinity Capture-Western
Reconstituted Complex
Co-crystal Structure
Protein-peptide
Co-crystal Structure
80 UBAP2L 9898
Reconstituted Complex Homo sapiens
81 CBL 867
Affinity Capture-Western Homo sapiens
82 HTT 3064
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
83 PLEKHB2 55041
Affinity Capture-MS Homo sapiens
84 TIGIT  
Affinity Capture-MS Homo sapiens
85 DNAJC5 80331
Proximity Label-MS Homo sapiens
86 DDX20 11218
Affinity Capture-Western Homo sapiens
87 UBA6 55236
Affinity Capture-MS Homo sapiens
88 PABPC1 26986
Reconstituted Complex Homo sapiens
89 FYN 2534
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
90 YAP1 10413
Affinity Capture-Western Homo sapiens
91 ARRDC2  
Affinity Capture-MS Homo sapiens
92 SARAF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 RORA  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
94 SMAD3 4088
Reconstituted Complex Homo sapiens
95 SMAD2 4087
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
96 ARRDC3 57561
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 KLF10  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
98 RAF1 5894
Affinity Capture-Western Homo sapiens
99 UBE2D3 7323
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
100 GNAI2 2771
Reconstituted Complex Homo sapiens
101 HNRNPL 3191
Reconstituted Complex Homo sapiens
102 MAPK8 5599
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
103 ITCH 83737
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
104 TMEM171  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
105 CEBPA  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
106 BIN1 274
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
107 CYLD  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
108 MLANA 2315
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
109 YOD1  
Affinity Capture-Western Homo sapiens
110 SGK3 23678
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
111 STAM 8027
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
112 SMARCC2 6601
Reconstituted Complex Homo sapiens
113 PCBP2 5094
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
114 PRRG4  
Reconstituted Complex Homo sapiens
115 FAM189B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
116 MARCH3 115123
Affinity Capture-MS Homo sapiens
117 MARVELD2 153562
Co-localization Homo sapiens
118 SNX18 112574
Affinity Capture-Western Homo sapiens
119 IPO5 3843
Co-fractionation Homo sapiens
120 CXCR4 7852
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
121 SF1 7536
Reconstituted Complex Homo sapiens
122 TAX1BP1 8887
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
123 ARRDC4  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
124 NDFIP2 54602
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
125 Ndfip1  
Affinity Capture-Western Mus musculus
126 RNF128  
Affinity Capture-MS Homo sapiens
127 UBE2L3 7332
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
128 NUMB 8650
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
129 UBE2L6 9246
Affinity Capture-MS Homo sapiens
130 CD274 29126
Affinity Capture-Western Homo sapiens
131 TRIM66  
Affinity Capture-MS Homo sapiens
132 TNFRSF1A 7132
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
133 TRAF6 7189
Affinity Capture-Western Homo sapiens
134 CUL3 8452
Affinity Capture-MS Homo sapiens
135 ERBB4 2066
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
136 RASSF1  
Affinity Capture-Western Homo sapiens
137 EGFR 1956
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
138 GLI1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
139 HNRNPUL1 11100
Reconstituted Complex Homo sapiens
140 DVL1 1855
Affinity Capture-Western Homo sapiens
141 POLR2A 5430
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
142 UBE2R2 54926
Reconstituted Complex Homo sapiens
143 SNX9 51429
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
144 NFE2  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Far Western Homo sapiens
145 MGRN1 23295
Affinity Capture-Western Homo sapiens
146 JUN 3725
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
147 PRKACA 5566
Biochemical Activity Homo sapiens
148 LDLRAD3  
Affinity Capture-Western Homo sapiens
149 SIK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
150 SLC3A2 6520
Affinity Capture-MS Homo sapiens
151 UBE2E1  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
152 RAB9A 9367
Proximity Label-MS Homo sapiens
153 TP63  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
154 PACSIN1  
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
Reconstituted Complex Homo sapiens
155 RPAP3 79657
Reconstituted Complex Homo sapiens
156 JUNB  
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
157 MLKL 197259
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
158 AMOT 154796
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
159 SMN1 6606
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
160 SMAD6  
Affinity Capture-MS Homo sapiens
161 UBE2D2 7322
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
162 ITSN1 6453
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
163 TRPV4 59341
Affinity Capture-Western Homo sapiens
164 MAP3K7  
Affinity Capture-Western Homo sapiens
165 ARAF 369
Affinity Capture-Western Homo sapiens
166 TP73  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
167 SH3GL2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
Reconstituted Complex Homo sapiens
168 MDM2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
169 TMEM92  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
170 NAA10 8260
Co-fractionation Homo sapiens
171 UBE2D1 7321
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
172 CBLC  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
173 GSN 2934
Affinity Capture-Western Homo sapiens
174 ARHGEF7 8874
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
175 BRAF  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
176 ARSH  
Affinity Capture-MS Homo sapiens
177 FBXL15  
Affinity Capture-Western Homo sapiens
178 NUDT21 11051
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
179 GGA3 23163
Affinity Capture-Western Homo sapiens
180 TMEM51 55092
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
181 ARRB2 409
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
182 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
183 TP53BP2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
184 SPOP  
Affinity Capture-Western Homo sapiens
185 ERGIC2 51290
Proximity Label-MS Homo sapiens
186 MAP2K4 6416
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
187 DTX3L 151636
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
188 PDE2A  
Affinity Capture-MS Homo sapiens
189 GRB2 2885
Co-fractionation Homo sapiens
190 SMAD7  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
191 RPAP2  
Reconstituted Complex Homo sapiens
192 CPSF6 11052
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
193 TP53 7157
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
194 WWOX 51741
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
195 PDCD6IP 10015
Affinity Capture-MS Homo sapiens
196 HDAC6 10013
Affinity Capture-MS Homo sapiens
197 SMARCC1 6599
Reconstituted Complex Homo sapiens
198 Pi4k2a  
Affinity Capture-Western Mus musculus
Biochemical Activity Mus musculus
Reconstituted Complex Mus musculus
199 NCOA1  
Affinity Capture-MS Homo sapiens
200 GLIS3  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
201 LITAF 9516
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
202 BID  
Affinity Capture-Western Homo sapiens
203 ILDR1 286676
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
204 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
205 COL18A1 80781
Affinity Capture-MS Homo sapiens
206 GBA 2629
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
207 SCAMP3 10067
Affinity Capture-MS Homo sapiens
208 SCNN1B 6338
Far Western Homo sapiens
209 ELOVL5 60481
Proximity Label-MS Homo sapiens
210 LATS1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
211 MTSS1 9788
Affinity Capture-Western Homo sapiens
212 PCNA 5111
Affinity Capture-MS Homo sapiens
213 WWP2 11060
Affinity Capture-MS Homo sapiens
214 TNFRSF10B 8795
Affinity Capture-Western Homo sapiens
215 NOTCH1 4851
Affinity Capture-Western Homo sapiens
216 BECN1 8678
Two-hybrid Homo sapiens
217 CGRRF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
218 LRRK2 120892
Affinity Capture-MS Homo sapiens
219 ARRB1 408
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
220 SH3GLB1 51100
Affinity Capture-Western Homo sapiens
221 GRAMD4 23151
Affinity Capture-Western Homo sapiens
222 RAB7A 7879
Proximity Label-MS Homo sapiens
223 STAM2 10254
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
224 SPG20 23111
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
225 FAM189A2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
226 EPHA2 1969
Proximity Label-MS Homo sapiens
227 TXNIP 10628
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
228 KRAS 3845
Proximity Label-MS Homo sapiens
229 UBE2Q2 92912
Reconstituted Complex Homo sapiens
230 ATXN3 4287
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
231 CUEDC1 404093
Affinity Capture-MS Homo sapiens
232 UBE2G1 7326
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
233 PTCH1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
234 IKBKB 3551
Affinity Capture-Western Homo sapiens
235 RPA3 6119
Proximity Label-MS Homo sapiens
236 CAV1 857
Affinity Capture-Western Homo sapiens
237 PRRG1 5638
Affinity Capture-MS Homo sapiens
238 N4BP1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which ITCH is involved
PathwayEvidenceSource
Activated NOTCH1 Transmits Signal to the Nucleus TAS Reactome
Activated NOTCH1 Transmits Signal to the Nucleus IEA Reactome
Adaptive Immune System TAS Reactome
Antigen processing: Ubiquitination & Proteasome degradation TAS Reactome
Class I MHC mediated antigen processing & presentation TAS Reactome
DDX58/IFIH1-mediated induction of interferon-alpha/beta TAS Reactome
Degradation of GLI1 by the proteasome TAS Reactome
Disease TAS Reactome
Downregulation of ERBB4 signaling TAS Reactome
Gene expression (Transcription) TAS Reactome
Generic Transcription Pathway TAS Reactome
Hedgehog 'off' state TAS Reactome
Hedgehog 'on' state TAS Reactome
Immune System TAS Reactome
Infectious disease TAS Reactome
Innate Immune System TAS Reactome
Negative regulators of DDX58/IFIH1 signaling TAS Reactome
NOD1/2 Signaling Pathway TAS Reactome
Nucleotide-binding domain, leucine rich repeat containing receptor (NLR) signaling pathways TAS Reactome
Programmed Cell Death TAS Reactome
Regulated Necrosis TAS Reactome
Regulation of necroptotic cell death TAS Reactome
RIPK1-mediated regulated necrosis TAS Reactome
RNA Polymerase II Transcription TAS Reactome
RUNX1 regulates transcription of genes involved in differentiation of HSCs TAS Reactome
SARS-CoV Infections TAS Reactome
SARS-CoV-1 activates/modulates innate immune responses TAS Reactome
SARS-CoV-1 Infection TAS Reactome
SARS-CoV-1-host interactions TAS Reactome
Signal Transduction TAS Reactome
Signal Transduction IEA Reactome
Signaling by ERBB4 TAS Reactome
Signaling by Hedgehog TAS Reactome
Signaling by NOTCH TAS Reactome
Signaling by NOTCH IEA Reactome
Signaling by NOTCH1 TAS Reactome
Signaling by NOTCH1 IEA Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Transcriptional regulation by RUNX1 TAS Reactome
Viral Infection Pathways TAS Reactome





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