Experiment description of studies that identified LAPTM5 in sEVs

1 Experiment ID 231 MISEV standards ✘ Biophysical techniques ✔ Alix|CD63|CD9 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Lipids Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 232 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 233 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for LAPTM5

Protein Interactor ExoCarta ID Identification method PubMed Species 1 BAG3 9531 Affinity Capture-MS Homo sapiens Affinity Capture-Luminescence Homo sapiens 2 TNFAIP3 7128 Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens Co-localization Homo sapiens Two-hybrid Homo sapiens 3 GYPA   Two-hybrid Homo sapiens 4 GGA3 23163 Affinity Capture-Western Homo sapiens 5 NEDD4 4734 Affinity Capture-Western Homo sapiens Biochemical Activity Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 6 REEP5 7905 Affinity Capture-MS Homo sapiens 7 TP53 7157 Synthetic Growth Defect Homo sapiens 8 WWP1 11059 Two-hybrid Homo sapiens 9 USP13   Two-hybrid Homo sapiens 10 LDLRAD1   Two-hybrid Homo sapiens 11 INSIG2   Two-hybrid Homo sapiens 12 KIR3DL3   Two-hybrid Homo sapiens 13 HEYL   Two-hybrid Homo sapiens 14 HMOX2 3163 Two-hybrid Homo sapiens 15 LAMP1 3916 Co-localization Homo sapiens 16 UBA52 7311 Two-hybrid Homo sapiens 17 EPS15 2060 Two-hybrid Homo sapiens 18 RTP2   Two-hybrid Homo sapiens 19 AIG1   Two-hybrid Homo sapiens 20 UBC 7316 Reconstituted Complex Homo sapiens 21 MAGI1   Affinity Capture-MS Homo sapiens 22 SELO 83642 Affinity Capture-MS Homo sapiens 23 CDC42 998 Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-MS Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens 24 YAP1 10413 Affinity Capture-MS Homo sapiens 25 WWP2 11060 Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 26 DCUN1D1 54165 Two-hybrid Homo sapiens 27 STXBP4 252983 Affinity Capture-MS Homo sapiens 28 STXBP1 6812 Affinity Capture-MS Homo sapiens 29 RNF168   Two-hybrid Homo sapiens 30 NEDD4L 23327 Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens 31 EPN3 55040 Two-hybrid Homo sapiens 32 EPN1 29924 Two-hybrid Homo sapiens 33 RPRM   Two-hybrid Homo sapiens 34 C4orf3   Two-hybrid Homo sapiens 35 HEY1   Two-hybrid Homo sapiens 36 ANKRD13D   Affinity Capture-MS Homo sapiens 37 VAMP1 6843 Two-hybrid Homo sapiens 38 SMURF2 64750 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 39 DISP1 84976 Two-hybrid Homo sapiens 40 CD63 967 Affinity Capture-MS Homo sapiens 41 CLDN19   Two-hybrid Homo sapiens 42 ITCH 83737 Affinity Capture-Western Homo sapiens Biochemical Activity Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 43 ANKRD13A 88455 Two-hybrid Homo sapiens 44 MRPL18 29074 Two-hybrid Homo sapiens 45 TOM1L2 146691 Two-hybrid Homo sapiens 46 UBE2J1 51465 Two-hybrid Homo sapiens 47 STXBP3 6814 Affinity Capture-MS Homo sapiens View the network

Pathways in which LAPTM5 is involved

No pathways found