Gene description for IFT140
Gene name intraflagellar transport 140
Gene symbol IFT140
Other names/aliases MZSDS
SRTD9
WDTC2
c305C8.4
c380F5.1
gs114
Species Homo sapiens
 Database cross references - IFT140
ExoCarta ExoCarta_9742
Vesiclepedia VP_9742
Entrez Gene 9742
HGNC 29077
MIM 614620
UniProt Q96RY7  
 IFT140 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for IFT140
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    determination of left/right symmetry GO:0007368 IEA
    heart development GO:0007507 IEA
    regulation of smoothened signaling pathway GO:0008589 IEA
    neural tube patterning GO:0021532 IEA
    embryonic camera-type eye development GO:0031076 IEA
    intraciliary retrograde transport GO:0035721 IBA
    intraciliary retrograde transport GO:0035721 IMP
    intraciliary retrograde transport GO:0035721 NAS
    photoreceptor cell outer segment organization GO:0035845 IEA
    embryonic digit morphogenesis GO:0042733 IEA
    embryonic cranial skeleton morphogenesis GO:0048701 IEA
    cilium assembly GO:0060271 IMP
    cilium assembly GO:0060271 NAS
    protein localization to cilium GO:0061512 IMP
    protein localization to cilium GO:0061512 IMP
    regulation of cilium assembly GO:1902017 IMP
    non-motile cilium assembly GO:1905515 IEA
    embryonic brain development GO:1990403 IEA
Subcellular Localization
    centrosome GO:0005813 IDA
    centriole GO:0005814 IEA
    cilium GO:0005929 IDA
    cilium GO:0005929 NAS
    cilium GO:0005929 TAS
    axoneme GO:0005930 IBA
    axoneme GO:0005930 ISS
    intraciliary transport particle A GO:0030991 IBA
    intraciliary transport particle A GO:0030991 IDA
    intraciliary transport particle A GO:0030991 IPI
    photoreceptor connecting cilium GO:0032391 IEA
    ciliary basal body GO:0036064 IBA
    ciliary basal body GO:0036064 IDA
    ciliary tip GO:0097542 TAS
    cone photoreceptor outer segment GO:0120199 IEA
 Experiment description of studies that identified IFT140 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for IFT140
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DNAJC7 7266
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 HDHD1  
Affinity Capture-MS Homo sapiens
3 FEZ1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 DHFR 1719
Affinity Capture-MS Homo sapiens
5 PFDN1 5201
Two-hybrid Homo sapiens
6 FAM167A  
Affinity Capture-MS Homo sapiens
7 FBXW11  
Affinity Capture-MS Homo sapiens
8 NPAS1  
Affinity Capture-MS Homo sapiens
9 NSUN5P1  
Affinity Capture-MS Homo sapiens
10 PRKY  
Affinity Capture-MS Homo sapiens
11 PPM1M  
Affinity Capture-MS Homo sapiens
12 FBXW5  
Affinity Capture-MS Homo sapiens
13 C9orf163  
Affinity Capture-MS Homo sapiens
14 CIB2  
Affinity Capture-MS Homo sapiens
15 TRIM10  
Affinity Capture-MS Homo sapiens
16 CTAG1B  
Affinity Capture-MS Homo sapiens
17 CRYL1 51084
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 TRIM66  
Affinity Capture-MS Homo sapiens
19 FAM96A  
Affinity Capture-MS Homo sapiens
20 SULT1C4  
Affinity Capture-MS Homo sapiens
21 PISD  
Affinity Capture-MS Homo sapiens
22 IFT122 55764
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 CUL3 8452
Affinity Capture-MS Homo sapiens
24 TFPT  
Affinity Capture-MS Homo sapiens
25 WDR35 57539
Affinity Capture-MS Homo sapiens
26 METTL21B  
Affinity Capture-MS Homo sapiens
27 DUSP16  
Affinity Capture-MS Homo sapiens
28 NTPCR 84284
Affinity Capture-MS Homo sapiens
29 PPP4R1L  
Affinity Capture-MS Homo sapiens
30 NEK2  
Two-hybrid Homo sapiens
31 ACSL3 2181
Affinity Capture-MS Homo sapiens
32 INF2 64423
Affinity Capture-MS Homo sapiens
33 SOHLH1  
Affinity Capture-MS Homo sapiens
34 C12orf74  
Affinity Capture-MS Homo sapiens
35 NT5C 30833
Affinity Capture-MS Homo sapiens
36 D2HGDH 728294
Affinity Capture-MS Homo sapiens
37 IFT43  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 PPARD  
Affinity Capture-MS Homo sapiens
39 PTGES3 10728
Affinity Capture-MS Homo sapiens
40 NUP210P1  
Affinity Capture-MS Homo sapiens
41 TULP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 WDR19 57728
Affinity Capture-MS Homo sapiens
43 C11orf74  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 TRMU  
Affinity Capture-MS Homo sapiens
45 NIPSNAP1 8508
Affinity Capture-MS Homo sapiens
46 HSPB8 26353
Affinity Capture-MS Homo sapiens
47 TTC21B 79809
Affinity Capture-MS Homo sapiens
48 RANBP2 5903
Two-hybrid Homo sapiens
49 TULP3 7289
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 UBXN6 80700
Affinity Capture-MS Homo sapiens
51 ATG7 10533
Affinity Capture-MS Homo sapiens
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