Gene description for Smarca5
Gene name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5
Gene symbol Smarca5
Other names/aliases 4933427E24Rik
D030040M08Rik
D330027N15Rik
MommeD4
Snf2h
Species Mus musculus
 Database cross references - Smarca5
ExoCarta ExoCarta_93762
Vesiclepedia VP_93762
Entrez Gene 93762
UniProt Q91ZW3  
 Smarca5 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Smarca5
Molecular Function
    DNA binding GO:0003677 IBA
    DNA binding GO:0003677 IEA
    DNA binding GO:0003677 ISO
    chromatin binding GO:0003682 IBA
    helicase activity GO:0004386 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    ATP hydrolysis activity GO:0016887 IEA
    ATP hydrolysis activity GO:0016887 ISO
    nucleosome binding GO:0031491 IEA
    histone binding GO:0042393 IEA
    histone binding GO:0042393 ISO
    ATP-dependent chromatin remodeler activity GO:0140658 ISO
    ATP-dependent chromatin remodeler activity GO:0140658 ISS
    nucleosome array spacer activity GO:0140750 IBA
    histone octamer slider activity GO:0140751 IEA
Biological Process
    rDNA heterochromatin formation GO:0000183 IDA
    regulation of DNA replication GO:0006275 ISO
    DNA repair GO:0006281 ISO
    DNA repair GO:0006281 ISS
    chromatin organization GO:0006325 IDA
    chromatin organization GO:0006325 ISO
    nucleosome assembly GO:0006334 ISO
    chromatin remodeling GO:0006338 IDA
    chromatin remodeling GO:0006338 IDA
    chromatin remodeling GO:0006338 IGI
    chromatin remodeling GO:0006338 ISO
    chromatin remodeling GO:0006338 NAS
    DNA methylation-dependent constitutive heterochromatin formation GO:0006346 IDA
    DNA-templated transcription initiation GO:0006352 IEA
    DNA-templated transcription initiation GO:0006352 ISO
    regulation of DNA-templated transcription GO:0006355 ISO
    DNA damage response GO:0006974 NAS
    embryo development GO:0009790 IMP
    negative regulation of transcription by RNA polymerase I GO:0016479 IDA
    heterochromatin formation GO:0031507 IBA
    heterochromatin formation GO:0031507 IDA
    positive regulation of DNA replication GO:0045740 IMP
    positive regulation of DNA replication GO:0045740 ISO
    positive regulation of DNA-templated transcription GO:0045893 IMP
    positive regulation of DNA-templated transcription GO:0045893 TAS
    positive regulation of transcription by RNA polymerase I GO:0045943 NAS
    positive regulation of transcription by RNA polymerase II GO:0045944 IBA
    positive regulation of transcription by RNA polymerase II GO:0045944 NAS
    positive regulation of transcription by RNA polymerase III GO:0045945 ISO
    antiviral innate immune response GO:0140374 IEA
    antiviral innate immune response GO:0140374 ISO
    negative regulation of mitotic chromosome condensation GO:1905213 ISO
    cellular response to leukemia inhibitory factor GO:1990830 IEP
Subcellular Localization
    chromatin GO:0000785 IBA
    condensed chromosome GO:0000793 IEA
    condensed chromosome GO:0000793 ISO
    fibrillar center GO:0001650 IEA
    fibrillar center GO:0001650 ISO
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    nucleoplasm GO:0005654 TAS
    chromatin silencing complex GO:0005677 IDA
    pericentric heterochromatin GO:0005721 IDA
    pericentric heterochromatin GO:0005721 ISO
    nucleolus GO:0005730 NAS
    CHRAC GO:0008623 NAS
    NURF complex GO:0016589 IEA
    NURF complex GO:0016589 ISO
    ACF complex GO:0016590 ISO
    ISWI-type complex GO:0031010 IPI
    RSF complex GO:0031213 ISO
    site of double-strand break GO:0035861 ISO
    site of double-strand break GO:0035861 ISS
    nuclear replication fork GO:0043596 IEA
    nuclear replication fork GO:0043596 ISO
    WICH complex GO:0090535 ISO
    NoRC complex GO:0090536 IPI
    CERF complex GO:0090537 IDA
    B-WICH complex GO:0110016 ISO
 Experiment description of studies that identified Smarca5 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Smarca5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Elf5  
Affinity Capture-MS Mus musculus
2 Esrrb  
Affinity Capture-MS Mus musculus
3 Pou5f1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
4 Cenpk  
Co-fractionation Mus musculus
5 Sin3a  
Co-fractionation Mus musculus
6 Baz2a  
Co-fractionation Mus musculus
Affinity Capture-MS Mus musculus
7 Gata1  
Affinity Capture-Western Mus musculus
8 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
9 Foxp3  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
10 Tfe3  
Affinity Capture-MS Mus musculus
11 Mxi1  
Two-hybrid Mus musculus
12 Chaf1a  
Affinity Capture-MS Mus musculus
13 Nde1  
Affinity Capture-Western Mus musculus
14 Kif4  
Co-fractionation Mus musculus
15 Nanog  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
16 Tfcp2l1  
Affinity Capture-MS Mus musculus
17 Rnf2  
Affinity Capture-MS Mus musculus
18 Zbtb7b  
Affinity Capture-MS Mus musculus
19 Eed  
Affinity Capture-MS Mus musculus
20 Baz1b 22385
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
21 Smc2 14211
Co-fractionation Mus musculus
22 Smc4 70099
Co-fractionation Mus musculus
23 Atg16l1  
Affinity Capture-MS Mus musculus
24 Baz1a  
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
Affinity Capture-Western Mus musculus
25 Satb1  
Co-fractionation Mus musculus
26 Fancd2  
Affinity Capture-MS Mus musculus
27 Kif23 71819
Co-fractionation Mus musculus
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