Gene description for TAPBP
Gene name TAP binding protein (tapasin)
Gene symbol TAPBP
Other names/aliases NGS17
TAPA
TPN
TPSN
Species Homo sapiens
 Database cross references - TAPBP
ExoCarta ExoCarta_6892
Vesiclepedia VP_6892
Entrez Gene 6892
HGNC 11566
MIM 601962
UniProt O15533  
 TAPBP identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for TAPBP
Molecular Function
    protein binding GO:0005515 IPI
    MHC class I protein complex binding GO:0023024 IBA
    MHC class I protein binding GO:0042288 TAS
    peptide antigen binding GO:0042605 TAS
    protein folding chaperone GO:0044183 IDA
    TAP1 binding GO:0046978 IDA
    TAP1 binding GO:0046978 IPI
    TAP1 binding GO:0046978 TAS
    TAP2 binding GO:0046979 IDA
    TAP2 binding GO:0046979 IPI
    TAP2 binding GO:0046979 TAS
    unfolded protein binding GO:0051082 TAS
    molecular adaptor activity GO:0060090 IDA
    TAP complex binding GO:0062061 IBA
    TAP complex binding GO:0062061 IDA
Biological Process
    MHC class Ib protein complex assembly GO:0002398 IMP
    peptide antigen assembly with MHC class I protein complex GO:0002502 IBA
    peptide antigen assembly with MHC class I protein complex GO:0002502 IDA
    protein folding GO:0006457 IEA
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 NAS
    regulation of gene expression GO:0010468 IMP
    antigen processing and presentation of endogenous peptide antigen via MHC class I GO:0019885 IEA
    peptide antigen stabilization GO:0050823 ISS
    regulation of protein complex stability GO:0061635 IDA
    protein-containing complex assembly GO:0065003 IDA
Subcellular Localization
    Golgi membrane GO:0000139 IDA
    endoplasmic reticulum GO:0005783 IC
    endoplasmic reticulum membrane GO:0005789 IDA
    phagocytic vesicle membrane GO:0030670 TAS
    endoplasmic reticulum-Golgi intermediate compartment membrane GO:0033116 TAS
    MHC class I peptide loading complex GO:0042824 IBA
    MHC class I peptide loading complex GO:0042824 IDA
    MHC class I peptide loading complex GO:0042824 NAS
    Tapasin-ERp57 complex GO:0061779 IPI
    lumenal side of endoplasmic reticulum membrane GO:0098553 TAS
 Experiment description of studies that identified TAPBP in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TAPBP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATG16L1 55054
Affinity Capture-MS Homo sapiens
2 HFE  
Affinity Capture-MS Homo sapiens
3 CALR 811
Affinity Capture-Western Homo sapiens
4 PRG2  
Affinity Capture-MS Homo sapiens
5 ISG15 9636
Affinity Capture-MS Homo sapiens
6 HLA-DPB1 3115
Affinity Capture-MS Homo sapiens
7 BTNL2  
Affinity Capture-MS Homo sapiens
8 TMEM106A  
Affinity Capture-MS Homo sapiens
9 KLK15  
Affinity Capture-MS Homo sapiens
10 HLA-B 3106
Affinity Capture-MS Homo sapiens
11 FAM20C 56975
Affinity Capture-MS Homo sapiens
12 HLA-DMB 3109
Affinity Capture-MS Homo sapiens
13 HCST  
Affinity Capture-MS Homo sapiens
14 CHST8 64377
Affinity Capture-MS Homo sapiens
15 GGH 8836
Affinity Capture-MS Homo sapiens
16 BRICD5  
Affinity Capture-MS Homo sapiens
17 Tap2  
Affinity Capture-Western Rattus norvegicus
18 PDIA3 2923
Affinity Capture-MS Homo sapiens
19 ECEL1  
Affinity Capture-MS Homo sapiens
20 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
21 GCGR  
Affinity Capture-MS Homo sapiens
22 UBC 7316
Reconstituted Complex Homo sapiens
23 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
24 HTR2C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 DDX58 23586
Affinity Capture-RNA Homo sapiens
26 COPG1 22820
Affinity Capture-Western Homo sapiens
27 LPAR1 1902
Affinity Capture-MS Homo sapiens
28 LYZL2  
Affinity Capture-MS Homo sapiens
29 NXF1 10482
Affinity Capture-RNA Homo sapiens
30 SFTPC  
Affinity Capture-MS Homo sapiens
31 COPB1 1315
Affinity Capture-Western Homo sapiens
32 PILRB 29990
Affinity Capture-MS Homo sapiens
33 ATP6V0C 527
Affinity Capture-MS Homo sapiens
34 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
35 CHRNB4  
Affinity Capture-MS Homo sapiens
36 MOV10 4343
Affinity Capture-RNA Homo sapiens
37 SDF2L1 23753
Affinity Capture-MS Homo sapiens
38 FBXO2 26232
Affinity Capture-MS Homo sapiens
39 B2M 567
Affinity Capture-Western Homo sapiens
40 LPAR2 9170
Affinity Capture-MS Homo sapiens
41 IDS  
Affinity Capture-MS Homo sapiens
42 GXYLT1 283464
Affinity Capture-MS Homo sapiens
43 CDHR4  
Affinity Capture-MS Homo sapiens
44 ASIC4  
Affinity Capture-MS Homo sapiens
45 VIPR1  
Affinity Capture-MS Homo sapiens
46 TAP1 6890
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
47 HLA-DQB1 3119
Affinity Capture-MS Homo sapiens
48 IL5RA  
Affinity Capture-MS Homo sapiens
49 HLA-A 3105
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
50 CLEC4E  
Affinity Capture-MS Homo sapiens
51 TAP2 6891
Reconstituted Complex Homo sapiens
52 HLA-C 3107
Affinity Capture-MS Homo sapiens
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