Gene description for HLA-DQB1
Gene name major histocompatibility complex, class II, DQ beta 1
Gene symbol HLA-DQB1
Other names/aliases CELIAC1
HLA-DQB
IDDM1
Species Homo sapiens
 Database cross references - HLA-DQB1
ExoCarta ExoCarta_3119
Entrez Gene 3119
HGNC 4944
MIM 604305
UniProt P01920  
 HLA-DQB1 identified in exosomes derived from the following tissue/cell type
B cells 12519789    
B cells 20458337    
B cells 20458337    
B cells 20458337    
Thymus 23844026    
 Gene ontology annotations for HLA-DQB1
Molecular Function
    MHC class II receptor activity GO:0032395 NAS
    peptide antigen binding GO:0042605 ISS
Biological Process
    cytokine-mediated signaling pathway GO:0019221 TAS
    humoral immune response mediated by circulating immunoglobulin GO:0002455 IDA
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 TAS
    T cell costimulation GO:0031295 TAS
    immune response GO:0006955 NAS
    interferon-gamma-mediated signaling pathway GO:0060333 TAS
    T cell receptor signaling pathway GO:0050852 TAS
    immunoglobulin production involved in immunoglobulin mediated immune response GO:0002381 IDA
Subcellular Localization
    lysosomal membrane GO:0005765 TAS
    transport vesicle membrane GO:0030658 TAS
    clathrin-coated endocytic vesicle membrane GO:0030669 TAS
    endosome membrane GO:0010008 IEA
    plasma membrane GO:0005886 TAS
    MHC class II protein complex GO:0042613 ISS
    Golgi membrane GO:0000139 TAS
    ER to Golgi transport vesicle membrane GO:0012507 TAS
    membrane GO:0016020 NAS
    integral component of lumenal side of endoplasmic reticulum membrane GO:0071556 TAS
    endocytic vesicle membrane GO:0030666 TAS
    trans-Golgi network membrane GO:0032588 TAS
 Experiment description of studies that identified HLA-DQB1 in exosomes
1
Experiment ID 11
ISEV standards
✘ EV Biophysical techniques
✔
HSC70|HSP90
EV Cytosolic markers
✔
MHCII|CD63|CD81
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
Western blotting
PubMed ID 12519789    
Organism Homo sapiens
Experiment description Proteomic and biochemical analyses of human B cell-derived exosomes. Potential implications for their function and multivesicular body formation.
Authors Wubbolts R, Leckie RS, Veenhuizen PT, Schwarzmann G, Mobius W, Hoernschemeyer J, Slot JW, Geuze HJ, Stoorvogel W
Journal name JBC
Publication year 2003
Sample B cells
Sample name RN (HLA-DR15+)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
Thin layer chromatography
HPLC
2
Experiment ID 79
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 80
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 81
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
5
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HLA-DQB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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