Gene description for HLA-DQB1
Gene name major histocompatibility complex, class II, DQ beta 1
Gene symbol HLA-DQB1
Other names/aliases CELIAC1
HLA-DQB
IDDM1
Species Homo sapiens
 Database cross references - HLA-DQB1
ExoCarta ExoCarta_3119
Vesiclepedia VP_3119
Entrez Gene 3119
HGNC 4944
MIM 604305
UniProt P01920  
 HLA-DQB1 identified in exosomes derived from the following tissue/cell type
B cells 12519789    
B cells 20458337    
B cells 20458337    
B cells 20458337    
Thymus 23844026    
 Gene ontology annotations for HLA-DQB1
Molecular Function
    protein binding GO:0005515 IPI
    MHC class II protein complex binding GO:0023026 IBA
    MHC class II receptor activity GO:0032395 NAS
    peptide antigen binding GO:0042605 IBA
    peptide antigen binding GO:0042605 ISS
Biological Process
    adaptive immune response GO:0002250 IEA
    peptide antigen assembly with MHC class II protein complex GO:0002503 IBA
    immune response GO:0006955 NAS
    humoral immune response GO:0006959 IMP
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 IBA
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 IDA
    positive regulation of immune response GO:0050778 IBA
    T cell receptor signaling pathway GO:0050852 IDA
    positive regulation of T cell activation GO:0050870 IBA
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    lysosomal membrane GO:0005765 IBA
    lysosomal membrane GO:0005765 TAS
    plasma membrane GO:0005886 TAS
    ER to Golgi transport vesicle membrane GO:0012507 TAS
    membrane GO:0016020 HDA
    membrane GO:0016020 NAS
    transport vesicle membrane GO:0030658 TAS
    endocytic vesicle membrane GO:0030666 TAS
    clathrin-coated endocytic vesicle membrane GO:0030669 TAS
    late endosome membrane GO:0031902 IBA
    trans-Golgi network membrane GO:0032588 TAS
    MHC class II protein complex GO:0042613 IBA
    MHC class II protein complex GO:0042613 ISS
    lumenal side of endoplasmic reticulum membrane GO:0098553 TAS
 Experiment description of studies that identified HLA-DQB1 in exosomes
1
Experiment ID 11
MISEV standards
Biophysical techniques
HSC70|HSP90|MHCII|CD63|CD81
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
Western blotting
PubMed ID 12519789    
Organism Homo sapiens
Experiment description Proteomic and biochemical analyses of human B cell-derived exosomes. Potential implications for their function and multivesicular body formation.
Authors "Wubbolts R, Leckie RS, Veenhuizen PT, Schwarzmann G, Mobius W, Hoernschemeyer J, Slot JW, Geuze HJ, Stoorvogel W"
Journal name JBC
Publication year 2003
Sample B cells
Sample name RN (HLA-DR15+)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
Thin layer chromatography
HPLC
2
Experiment ID 79
MISEV standards
Biophysical techniques
CD81|MHCII
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 80
MISEV standards
Biophysical techniques
CD81|MHCII
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 81
MISEV standards
Biophysical techniques
CD81|MHCII
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
5
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HLA-DQB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SGCB 6443
Affinity Capture-MS Homo sapiens
2 INTS4  
Affinity Capture-MS Homo sapiens
3 HEATR6  
Affinity Capture-MS Homo sapiens
4 NDUFB8 4714
Affinity Capture-MS Homo sapiens
5 BTNL2  
Affinity Capture-MS Homo sapiens
6 TMEM106A  
Affinity Capture-MS Homo sapiens
7 NAAA  
Affinity Capture-MS Homo sapiens
8 TMEM30A 55754
Affinity Capture-MS Homo sapiens
9 NCSTN 23385
Affinity Capture-MS Homo sapiens
10 FANCD2  
Affinity Capture-MS Homo sapiens
11 ECEL1  
Affinity Capture-MS Homo sapiens
12 TMEM87A 25963
Affinity Capture-MS Homo sapiens
13 HLA-DMB 3109
Affinity Capture-MS Homo sapiens
14 HLA-G 3135
Affinity Capture-MS Homo sapiens
15 NDUFA9 4704
Affinity Capture-MS Homo sapiens
16 CLSTN3 9746
Affinity Capture-MS Homo sapiens
17 HLA-DRA 3122
Affinity Capture-MS Homo sapiens
18 GGH 8836
Affinity Capture-MS Homo sapiens
19 PON2 5445
Affinity Capture-MS Homo sapiens
20 CLEC2D  
Affinity Capture-MS Homo sapiens
21 MAN1B1 11253
Affinity Capture-MS Homo sapiens
22 NOMO1 23420
Affinity Capture-MS Homo sapiens
23 CD1A 909
Affinity Capture-MS Homo sapiens
24 CNNM4 26504
Affinity Capture-MS Homo sapiens
25 PI15  
Affinity Capture-MS Homo sapiens
26 ITM2C 81618
Affinity Capture-MS Homo sapiens
27 NDUFA12 55967
Affinity Capture-MS Homo sapiens
28 SSBP2  
Affinity Capture-MS Homo sapiens
29 ITGA6 3655
Affinity Capture-MS Homo sapiens
30 GBF1 8729
Affinity Capture-MS Homo sapiens
31 APOM 55937
Affinity Capture-MS Homo sapiens
32 TUBA1A 7846
Affinity Capture-MS Homo sapiens
33 SFTPC  
Affinity Capture-MS Homo sapiens
34 INTS8  
Affinity Capture-MS Homo sapiens
35 CNNM3 26505
Affinity Capture-MS Homo sapiens
36 TAPBP 6892
Affinity Capture-MS Homo sapiens
37 NDUFB9 4715
Affinity Capture-MS Homo sapiens
38 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
39 SIRPD  
Affinity Capture-MS Homo sapiens
40 IDS  
Affinity Capture-MS Homo sapiens
41 HLA-DRB3 3125
Affinity Capture-MS Homo sapiens
42 HLA-DPA1  
Affinity Capture-MS Homo sapiens
43 ATP2A1 487
Affinity Capture-MS Homo sapiens
44 KCNJ8  
Affinity Capture-MS Homo sapiens
45 SARAF  
Affinity Capture-MS Homo sapiens
46 UGT1A7 54577
Affinity Capture-MS Homo sapiens
47 POLD3  
Affinity Capture-MS Homo sapiens
48 HLA-F 3134
Affinity Capture-MS Homo sapiens
49 HLA-B 3106
Affinity Capture-MS Homo sapiens
50 MOGS 7841
Affinity Capture-MS Homo sapiens
51 DNAJB9 4189
Affinity Capture-MS Homo sapiens
52 MROH1 727957
Affinity Capture-MS Homo sapiens
53 NR2F2  
Affinity Capture-MS Homo sapiens
54 GXYLT1 283464
Affinity Capture-MS Homo sapiens
55 ASIC4  
Affinity Capture-MS Homo sapiens
56 SEMA4F  
Affinity Capture-MS Homo sapiens
57 DNASE1L1 1774
Affinity Capture-MS Homo sapiens
58 C9orf72  
Affinity Capture-MS Homo sapiens
59 NCAPG2 54892
Affinity Capture-MS Homo sapiens
60 TMEM106C 79022
Affinity Capture-MS Homo sapiens
61 SLC25A4 291
Affinity Capture-MS Homo sapiens
62 HLA-DRB1 3123
Affinity Capture-MS Homo sapiens
63 SLC1A1 6505
Affinity Capture-MS Homo sapiens
64 HEATR1 55127
Affinity Capture-MS Homo sapiens
65 HLA-A 3105
Affinity Capture-MS Homo sapiens
66 NXPH1  
Affinity Capture-MS Homo sapiens
67 SDF2L1 23753
Affinity Capture-MS Homo sapiens
68 FANCI 55215
Affinity Capture-MS Homo sapiens
69 MON2 23041
Affinity Capture-MS Homo sapiens
70 CPT1A 1374
Affinity Capture-MS Homo sapiens
71 PIPSL 266971
Affinity Capture-MS Homo sapiens
72 HLA-C 3107
Affinity Capture-MS Homo sapiens
73 KIAA0922  
Affinity Capture-MS Homo sapiens
74 MAN1A1 4121
Affinity Capture-MS Homo sapiens
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