Gene description for ARAP3
Gene name ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3
Gene symbol ARAP3
Other names/aliases CENTD3
DRAG1
Species Homo sapiens
 Database cross references - ARAP3
ExoCarta ExoCarta_64411
Vesiclepedia VP_64411
Entrez Gene 64411
HGNC 24097
MIM 606647
UniProt Q8WWN8  
 ARAP3 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for ARAP3
Molecular Function
    GTPase activator activity GO:0005096 ISS
    protein binding GO:0005515 IPI
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 IBA
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 IDA
    phosphatidylinositol-3,4-bisphosphate binding GO:0043325 IDA
    metal ion binding GO:0046872 IEA
Biological Process
    cytoskeleton organization GO:0007010 TAS
    signal transduction GO:0007165 IEA
    vesicle-mediated transport GO:0016192 TAS
    regulation of actin cytoskeleton organization GO:0032956 IBA
Subcellular Localization
    ruffle GO:0001726 IEA
    cytoplasm GO:0005737 IBA
    cytoskeleton GO:0005856 IEA
    plasma membrane GO:0005886 IEA
    lamellipodium GO:0030027 IEA
 Experiment description of studies that identified ARAP3 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ARAP3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZNF252P-AS1  
Affinity Capture-MS Homo sapiens
2 SENP3 26168
Affinity Capture-MS Homo sapiens
3 NPAS1  
Affinity Capture-MS Homo sapiens
4 NPRL2  
Affinity Capture-MS Homo sapiens
5 SWSAP1  
Affinity Capture-MS Homo sapiens
6 PSG11  
Affinity Capture-MS Homo sapiens
7 CIB2  
Affinity Capture-MS Homo sapiens
8 XPO1 7514
Affinity Capture-MS Homo sapiens
9 D2HGDH 728294
Affinity Capture-MS Homo sapiens
10 C12orf74  
Affinity Capture-MS Homo sapiens
11 FAM96A  
Affinity Capture-MS Homo sapiens
12 RFXANK  
Affinity Capture-MS Homo sapiens
13 SULT1C4  
Affinity Capture-MS Homo sapiens
14 ASB6 140459
Affinity Capture-MS Homo sapiens
15 PPARD  
Affinity Capture-MS Homo sapiens
16 PLBD1 79887
Affinity Capture-MS Homo sapiens
17 DEF8  
Affinity Capture-MS Homo sapiens
18 METTL21B  
Affinity Capture-MS Homo sapiens
19 HES6  
Affinity Capture-MS Homo sapiens
20 HDHD1  
Affinity Capture-MS Homo sapiens
21 Myh10 77579
Affinity Capture-MS Mus musculus
22 ALG1L  
Affinity Capture-MS Homo sapiens
23 CRYBB3  
Affinity Capture-MS Homo sapiens
24 DHFR 1719
Affinity Capture-MS Homo sapiens
25 INF2 64423
Affinity Capture-MS Homo sapiens
26 LRRC29  
Affinity Capture-MS Homo sapiens
27 TUBB 203068
Affinity Capture-MS Homo sapiens
28 NT5C 30833
Affinity Capture-MS Homo sapiens
29 NTRK1 4914
Affinity Capture-MS Homo sapiens
30 CIB3  
Affinity Capture-MS Homo sapiens
31 TRIM35 23087
Affinity Capture-MS Homo sapiens
32 SSSCA1 10534
Affinity Capture-MS Homo sapiens
33 CLASRP  
Affinity Capture-MS Homo sapiens
34 ASCL4  
Affinity Capture-MS Homo sapiens
35 B4GALT2  
Affinity Capture-MS Homo sapiens
36 SH3KBP1 30011
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
37 NFKBIB  
Affinity Capture-MS Homo sapiens
38 ASAP1 50807
Affinity Capture-Western Homo sapiens
39 DNAI2  
Affinity Capture-MS Homo sapiens
40 SF3B3 23450
Affinity Capture-MS Homo sapiens
41 HSPB8 26353
Affinity Capture-MS Homo sapiens
42 UBXN6 80700
Affinity Capture-MS Homo sapiens
43 ATG7 10533
Affinity Capture-MS Homo sapiens
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