Gene description for Rab11a
Gene name RAB11A, member RAS oncogene family
Gene symbol Rab11a
Other names/aliases -
Species Mus musculus
 Database cross references - Rab11a
ExoCarta ExoCarta_53869
Vesiclepedia VP_53869
Entrez Gene 53869
UniProt P62492  
 Rab11a identified in sEVs derived from the following tissue/cell type
Embryonic fibroblasts 18494037    
Microglia 16081791    
Pancreatic cells 19351151    
 Gene ontology annotations for Rab11a
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 ISO
    GTPase activity GO:0003924 ISS
    G protein activity GO:0003925 IEA
    G protein activity GO:0003925 ISO
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IEA
    GTP binding GO:0005525 ISO
    microtubule binding GO:0008017 IEA
    microtubule binding GO:0008017 ISO
    myosin V binding GO:0031489 IEA
    myosin V binding GO:0031489 ISO
    dynein light intermediate chain binding GO:0051959 ISO
    dynein light intermediate chain binding GO:0051959 ISS
Biological Process
    exocytosis GO:0006887 IBA
    mitotic metaphase chromosome alignment GO:0007080 IEA
    mitotic metaphase chromosome alignment GO:0007080 ISO
    positive regulation of epithelial cell migration GO:0010634 IEA
    positive regulation of epithelial cell migration GO:0010634 ISO
    positive regulation of G2/M transition of mitotic cell cycle GO:0010971 IEA
    positive regulation of G2/M transition of mitotic cell cycle GO:0010971 ISO
    vesicle-mediated transport GO:0016192 ISO
    astral microtubule organization GO:0030953 IEA
    astral microtubule organization GO:0030953 ISO
    neuron projection development GO:0031175 ISO
    neuron projection development GO:0031175 ISS
    melanosome transport GO:0032402 IBA
    melanosome transport GO:0032402 IMP
    regulation of cytokinesis GO:0032465 ISO
    protein localization to cell surface GO:0034394 IMP
    multivesicular body assembly GO:0036258 IEA
    multivesicular body assembly GO:0036258 ISO
    regulation of long-term neuronal synaptic plasticity GO:0048169 ISO
    regulation of protein transport GO:0051223 ISO
    establishment of vesicle localization GO:0051650 ISO
    regulation of vesicle-mediated transport GO:0060627 ISO
    early endosome to recycling endosome transport GO:0061502 IEA
    early endosome to recycling endosome transport GO:0061502 ISO
    protein localization to cilium GO:0061512 ISO
    protein localization to cilium GO:0061512 ISS
    protein transmembrane transport GO:0071806 IMP
    establishment of protein localization to organelle GO:0072594 IEA
    establishment of protein localization to organelle GO:0072594 ISO
    protein localization to plasma membrane GO:0072659 ISO
    protein localization to plasma membrane GO:0072659 ISS
    establishment of protein localization to membrane GO:0090150 IEA
    establishment of protein localization to membrane GO:0090150 ISO
    mitotic spindle assembly GO:0090307 IEA
    mitotic spindle assembly GO:0090307 ISO
    neurotransmitter receptor transport, endosome to postsynaptic membrane GO:0098887 IBA
    neurotransmitter receptor transport, endosome to postsynaptic membrane GO:0098887 ISO
    vesicle-mediated transport in synapse GO:0099003 ISO
    amyloid-beta clearance by transcytosis GO:0150093 IEA
    amyloid-beta clearance by transcytosis GO:0150093 ISO
    regulation of cilium assembly GO:1902017 ISO
    regulation of cilium assembly GO:1902017 ISS
    regulation of early endosome to recycling endosome transport GO:1902954 ISO
    regulation of early endosome to recycling endosome transport GO:1902954 ISS
    positive regulation of protein localization to plasma membrane GO:1903078 ISO
    positive regulation of mitotic cytokinetic process GO:1903438 IEA
    positive regulation of mitotic cytokinetic process GO:1903438 ISO
    regulation of protein localization to centrosome GO:1904779 ISO
    regulation of protein localization to centrosome GO:1904779 ISS
    exosomal secretion GO:1990182 IEA
    exosomal secretion GO:1990182 ISO
    regulation of endocytic recycling GO:2001135 ISO
    regulation of endocytic recycling GO:2001135 ISS
Subcellular Localization
    Golgi membrane GO:0000139 ISO
    Golgi membrane GO:0000139 ISS
    spindle pole GO:0000922 IEA
    spindle pole GO:0000922 ISO
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 HDA
    multivesicular body GO:0005771 IEA
    multivesicular body GO:0005771 ISO
    Golgi apparatus GO:0005794 IBA
    trans-Golgi network GO:0005802 ISO
    centrosome GO:0005813 ISO
    centrosome GO:0005813 ISO
    centrosome GO:0005813 ISS
    centriole GO:0005814 IDA
    kinetochore microtubule GO:0005828 IEA
    kinetochore microtubule GO:0005828 ISO
    membrane GO:0016020 IDA
    transport vesicle GO:0030133 IBA
    endocytic vesicle membrane GO:0030666 ISO
    endocytic vesicle membrane GO:0030666 ISS
    cytoplasmic vesicle GO:0031410 ISO
    vesicle GO:0031982 ISO
    cleavage furrow GO:0032154 ISO
    cleavage furrow GO:0032154 ISS
    trans-Golgi network membrane GO:0032588 ISO
    trans-Golgi network membrane GO:0032588 ISS
    protein-containing complex GO:0032991 IEA
    protein-containing complex GO:0032991 ISO
    centriolar satellite GO:0034451 IEA
    centriolar satellite GO:0034451 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    phagocytic vesicle GO:0045335 ISO
    phagocytic vesicle GO:0045335 ISS
    recycling endosome GO:0055037 IDA
    recycling endosome GO:0055037 ISO
    recycling endosome membrane GO:0055038 IEA
    extracellular exosome GO:0070062 IEA
    extracellular exosome GO:0070062 ISO
    Schaffer collateral - CA1 synapse GO:0098685 ISO
    postsynaptic recycling endosome GO:0098837 IBA
    postsynaptic recycling endosome GO:0098837 IEA
    postsynaptic recycling endosome GO:0098837 ISO
    postsynaptic recycling endosome GO:0098837 ISO
    glutamatergic synapse GO:0098978 IEA
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Rab11a in sEVs
1
Experiment ID 73
MISEV standards
EM
Biophysical techniques
HSP70|HSP90
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
2
Experiment ID 14
MISEV standards
EM
Biophysical techniques
RAB7|RAB11|CD9|CD63|LAMP1|LAMP2
Enriched markers
DNM
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors "Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L"
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
3
Experiment ID 188
MISEV standards
EM
Biophysical techniques
GAPDH|UCHL1|HSP90
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Rab11a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAB11FIP1 80223
Affinity Capture-MS Homo sapiens
2 PHB 5245
Affinity Capture-MS Homo sapiens
3 RAB11B 9230
Affinity Capture-MS Homo sapiens
4 EVI5  
Affinity Capture-MS Homo sapiens
5 Sema4a  
Affinity Capture-Western Mus musculus
6 RAB11FIP2  
Affinity Capture-MS Homo sapiens
7 WDR44 54521
Affinity Capture-MS Homo sapiens
8 PHB2 11331
Affinity Capture-MS Homo sapiens
9 Rab11a 53869
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
10 CHEK2  
Affinity Capture-MS Homo sapiens
11 Optn  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
12 Fancd2  
Affinity Capture-MS Mus musculus
13 SLC25A5 292
Affinity Capture-MS Homo sapiens
14 EVI5L  
Affinity Capture-MS Homo sapiens
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