Gene description for Crk
Gene name v-crk sarcoma virus CT10 oncogene homolog (avian)
Gene symbol Crk
Other names/aliases Crk-I
Crk-II
Crk-III
Crk3
CrkIII
Crko
c-Crk
p38
Species Mus musculus
 Database cross references - Crk
ExoCarta ExoCarta_12928
Vesiclepedia VP_12928
Entrez Gene 12928
UniProt Q64010  
 Crk identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Crk
Molecular Function
    phosphotyrosine residue binding GO:0001784 IEA
    phosphotyrosine residue binding GO:0001784 ISO
    insulin-like growth factor receptor binding GO:0005159 IEA
    insulin-like growth factor receptor binding GO:0005159 ISO
    protein binding GO:0005515 IPI
    cytoskeletal protein binding GO:0008092 IEA
    cytoskeletal protein binding GO:0008092 ISO
    SH3 domain binding GO:0017124 IEA
    SH3 domain binding GO:0017124 ISO
    enzyme binding GO:0019899 ISO
    kinase binding GO:0019900 ISO
    protein domain specific binding GO:0019904 ISO
    signaling receptor complex adaptor activity GO:0030159 IPI
    protein-macromolecule adaptor activity GO:0030674 ISO
    receptor tyrosine kinase binding GO:0030971 IBA
    ubiquitin protein ligase binding GO:0031625 IPI
    signaling adaptor activity GO:0035591 IBA
    signaling adaptor activity GO:0035591 IDA
    signaling adaptor activity GO:0035591 ISO
    SH2 domain binding GO:0042169 IEA
    SH2 domain binding GO:0042169 ISO
    protein phosphorylated amino acid binding GO:0045309 IDA
    protein phosphorylated amino acid binding GO:0045309 ISO
    ephrin receptor binding GO:0046875 IPI
    ephrin receptor binding GO:0046875 ISO
    scaffold protein binding GO:0097110 IEA
    scaffold protein binding GO:0097110 ISO
    protein tyrosine kinase binding GO:1990782 IEA
    protein tyrosine kinase binding GO:1990782 ISO
Biological Process
    neuron migration GO:0001764 IGI
    response to yeast GO:0001878 IEA
    regulation of leukocyte migration GO:0002685 IGI
    lipid metabolic process GO:0006629 IGI
    enzyme-linked receptor protein signaling pathway GO:0007167 IBA
    enzyme-linked receptor protein signaling pathway GO:0007167 IDA
    enzyme-linked receptor protein signaling pathway GO:0007167 IGI
    enzyme-linked receptor protein signaling pathway GO:0007167 IMP
    cell population proliferation GO:0008283 IGI
    regulation of cell shape GO:0008360 ISO
    regulation of cell shape GO:0008360 ISS
    regulation of signal transduction GO:0009966 ISO
    positive regulation of smooth muscle cell migration GO:0014911 IEA
    positive regulation of smooth muscle cell migration GO:0014911 ISO
    dendrite development GO:0016358 IGI
    cell migration GO:0016477 IBA
    hippocampus development GO:0021766 IGI
    cerebral cortex development GO:0021987 IGI
    establishment of cell polarity GO:0030010 IGI
    actin cytoskeleton organization GO:0030036 ISO
    actin cytoskeleton organization GO:0030036 ISS
    positive regulation of cell growth GO:0030307 ISO
    regulation of actin cytoskeleton organization GO:0032956 IGI
    regulation of actin cytoskeleton organization GO:0032956 ISO
    regulation of actin cytoskeleton organization GO:0032956 ISS
    regulation of cell adhesion mediated by integrin GO:0033628 IGI
    regulation of Rac protein signal transduction GO:0035020 ISO
    positive regulation of Rac protein signal transduction GO:0035022 IMP
    helper T cell diapedesis GO:0035685 IGI
    response to hepatocyte growth factor GO:0035728 IEA
    response to hepatocyte growth factor GO:0035728 ISO
    reelin-mediated signaling pathway GO:0038026 IGI
    response to hydrogen peroxide GO:0042542 IEA
    response to hydrogen peroxide GO:0042542 ISO
    regulation of GTPase activity GO:0043087 ISO
    regulation of GTPase activity GO:0043087 ISS
    negative regulation of natural killer cell mediated cytotoxicity GO:0045953 IEA
    negative regulation of natural killer cell mediated cytotoxicity GO:0045953 ISO
    positive regulation of JNK cascade GO:0046330 IEA
    positive regulation of JNK cascade GO:0046330 ISO
    ephrin receptor signaling pathway GO:0048013 ISO
    ephrin receptor signaling pathway GO:0048013 ISS
    regulation of dendrite development GO:0050773 IGI
    cell chemotaxis GO:0060326 IGI
    negative regulation of wound healing GO:0061045 IEA
    negative regulation of wound healing GO:0061045 ISO
    response to cholecystokinin GO:0061847 IEA
    response to cholecystokinin GO:0061847 ISO
    cellular response to transforming growth factor beta stimulus GO:0071560 IEA
    cellular response to transforming growth factor beta stimulus GO:0071560 ISO
    cellular response to nitric oxide GO:0071732 IEA
    cellular response to nitric oxide GO:0071732 ISO
    protein localization to membrane GO:0072657 IEA
    protein localization to membrane GO:0072657 ISO
    activation of GTPase activity GO:0090630 IGI
    postsynaptic specialization assembly GO:0098698 IDA
    postsynaptic specialization assembly GO:0098698 IMP
    cerebellar neuron development GO:0098749 IGI
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 ISO
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 ISS
    response to peptide GO:1901652 IEA
    response to peptide GO:1901652 ISO
    regulation of intracellular signal transduction GO:1902531 ISO
    positive regulation of skeletal muscle acetylcholine-gated channel clustering GO:1904395 IDA
    positive regulation of skeletal muscle acetylcholine-gated channel clustering GO:1904395 IGI
    positive regulation of skeletal muscle acetylcholine-gated channel clustering GO:1904395 IMP
    cellular response to nerve growth factor stimulus GO:1990090 IEA
    cellular response to nerve growth factor stimulus GO:1990090 ISO
    cellular response to insulin-like growth factor stimulus GO:1990314 IEA
    cellular response to insulin-like growth factor stimulus GO:1990314 ISO
    cellular response to endothelin GO:1990859 IEA
    cellular response to endothelin GO:1990859 ISO
    negative regulation of cell motility GO:2000146 IEA
    negative regulation of cell motility GO:2000146 ISO
    regulation of T cell migration GO:2000404 IGI
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    plasma membrane GO:0005886 IEA
    actin cytoskeleton GO:0015629 IDA
    membrane GO:0016020 IMP
    neuromuscular junction GO:0031594 IDA
    neuromuscular junction GO:0031594 IMP
    protein-containing complex GO:0032991 IEA
    protein-containing complex GO:0032991 ISO
 Experiment description of studies that identified Crk in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Crk
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Pik3r2  
Affinity Capture-MS Mus musculus
2 Arhgap33  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
3 Stam2  
Affinity Capture-MS Mus musculus
4 Pik3r1  
Affinity Capture-MS Mus musculus
5 D8Ertd82e  
Affinity Capture-MS Mus musculus
6 Pik3cb  
Affinity Capture-MS Mus musculus
7 Atxn1  
Proximity Label-MS Mus musculus
8 Dvl2  
Affinity Capture-Western Mus musculus
9 Ywhaz 22631
Affinity Capture-MS Mus musculus
10 Ywhaq 22630
Affinity Capture-MS Mus musculus
11 Nedd9  
Affinity Capture-MS Mus musculus
12 Ywhab 54401
Affinity Capture-MS Mus musculus
13 Avil 11567
Affinity Capture-MS Mus musculus
14 Ywhah 22629
Affinity Capture-MS Mus musculus
15 Abl1  
Affinity Capture-MS Mus musculus
16 Ywhag 22628
Affinity Capture-MS Mus musculus
17 Cblb 208650
Affinity Capture-Western Mus musculus
18 Pxn  
Affinity Capture-Western Mus musculus
19 Bcr  
Affinity Capture-MS Mus musculus
20 Ywhae 22627
Affinity Capture-MS Mus musculus
21 Rapgef1  
Affinity Capture-MS Mus musculus
22 Cbl 12402
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
23 Plec 18810
Affinity Capture-MS Mus musculus
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