Gene description for Ywhaq
Gene name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
Gene symbol Ywhaq
Other names/aliases 2700028P07Rik
AA409740
AU021156
R74690
Species Mus musculus
 Database cross references - Ywhaq
ExoCarta ExoCarta_22630
Vesiclepedia VP_22630
Entrez Gene 22630
UniProt P68254  
 Ywhaq identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
Oligodendrocytes 21136642    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ywhaq
Molecular Function
    protein binding GO:0005515 IPI
    protein domain specific binding GO:0019904 IDA
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    transmembrane transporter binding GO:0044325 IEA
    transmembrane transporter binding GO:0044325 ISO
    14-3-3 protein binding GO:0071889 IEA
    14-3-3 protein binding GO:0071889 ISO
Biological Process
    protein targeting GO:0006605 IDA
    signal transduction GO:0007165 IBA
    signal transduction GO:0007165 TAS
    small GTPase-mediated signal transduction GO:0007264 IPI
    protein localization GO:0008104 IBA
    negative regulation of monoatomic ion transmembrane transport GO:0034766 IEA
    negative regulation of monoatomic ion transmembrane transport GO:0034766 ISO
    negative regulation of DNA-templated transcription GO:0045892 IEA
    negative regulation of DNA-templated transcription GO:0045892 ISO
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 TAS
    protein-containing complex GO:0032991 ISO
    synapse GO:0045202 IEA
    synapse GO:0045202 ISO
 Experiment description of studies that identified Ywhaq in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
5
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ywhaq
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Pfkfb2  
Co-fractionation Mus musculus
2 Rmdn3  
Co-fractionation Mus musculus
3 Kcnma1  
Affinity Capture-MS Mus musculus
4 Hsp90ab1 15516
Co-fractionation Mus musculus
5 Sipa1l3  
Co-fractionation Mus musculus
6 Trim32  
Affinity Capture-Western Mus musculus
7 Ywhah 22629
Co-fractionation Mus musculus
8 HIST1H3A 8350
Affinity Capture-MS Homo sapiens
9 Dlgap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
10 Tsc1  
Affinity Capture-MS Mus musculus
11 Atxn1  
Proximity Label-MS Mus musculus
12 Hdgf  
Co-fractionation Mus musculus
13 Kctd13  
Affinity Capture-MS Mus musculus
14 Tfe3  
Affinity Capture-MS Mus musculus
15 Rpgrip1l  
Affinity Capture-MS Mus musculus
16 Lrrk2  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
17 Api5  
Co-fractionation Mus musculus
18 Ywhae 22627
Co-fractionation Mus musculus
19 Emd  
Co-fractionation Mus musculus
20 Hsp90aa1 15519
Co-fractionation Mus musculus
21 Slc8a2  
Co-fractionation Mus musculus
22 Pfdn2  
Co-fractionation Mus musculus
23 RGS3 5998
Two-hybrid Homo sapiens
24 Braf 109880
Two-hybrid Mus musculus
25 Anxa1 16952
Co-fractionation Mus musculus
26 Ywhab 54401
Co-fractionation Mus musculus
27 Nedd4l 83814
Affinity Capture-Western Mus musculus
28 Crk 12928
Affinity Capture-MS Mus musculus
29 Lnx1  
Affinity Capture-MS Mus musculus
30 Hnrnpk 15387
Co-fractionation Mus musculus
31 Ywhaz 22631
Co-fractionation Mus musculus
32 Atg16l1  
Affinity Capture-MS Mus musculus
33 Ywhag 22628
Co-fractionation Mus musculus
34 Fancd2  
Affinity Capture-MS Mus musculus
35 Tcf3  
Affinity Capture-MS Mus musculus
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