Gene description for MAN2C1
Gene name mannosidase, alpha, class 2C, member 1
Gene symbol MAN2C1
Other names/aliases MAN6A8
MANA
MANA1
Species Homo sapiens
 Database cross references - MAN2C1
ExoCarta ExoCarta_4123
Vesiclepedia VP_4123
Entrez Gene 4123
HGNC 6827
MIM 154580
UniProt Q9NTJ4  
 MAN2C1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for MAN2C1
Molecular Function
    alpha-mannosidase activity GO:0004559 IBA
    carbohydrate binding GO:0030246 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    mannose metabolic process GO:0006013 IEA
    oligosaccharide catabolic process GO:0009313 IBA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified MAN2C1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MAN2C1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CLLU1OS  
Affinity Capture-MS Homo sapiens
2 EXOSC6 118460
Two-hybrid Homo sapiens
3 SNCA 6622
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 PRRC2A 7916
Two-hybrid Homo sapiens
5 HSPA8 3312
Affinity Capture-MS Homo sapiens
6 HOXA7  
Affinity Capture-MS Homo sapiens
7 PCMT1 5110
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 C9orf142  
Affinity Capture-MS Homo sapiens
9 HIST1H1D 3007
Affinity Capture-MS Homo sapiens
10 ASPSCR1 79058
Affinity Capture-MS Homo sapiens
11 NLGN3  
Two-hybrid Homo sapiens
12 BRCA1 672
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
13 HOXD11  
Affinity Capture-MS Homo sapiens
14 CTTN 2017
Affinity Capture-MS Homo sapiens
15 SRA1 10011
Affinity Capture-MS Homo sapiens
16 VWA5A 4013
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 SRSF2 6427
Affinity Capture-MS Homo sapiens
18 EGFLAM  
Affinity Capture-MS Homo sapiens
19 TPPP 11076
Affinity Capture-MS Homo sapiens
20 DDX58 23586
Affinity Capture-RNA Homo sapiens
21 XRN1 54464
Two-hybrid Homo sapiens
22 SGTB  
Affinity Capture-MS Homo sapiens
23 DHFR 1719
Affinity Capture-MS Homo sapiens
24 RIPPLY3  
Affinity Capture-MS Homo sapiens
25 NUP210P1  
Affinity Capture-MS Homo sapiens
26 KRT2 3849
Affinity Capture-MS Homo sapiens
27 CDC20B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 TEX33  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 ZC2HC1C  
Affinity Capture-MS Homo sapiens
30 DAP  
Affinity Capture-MS Homo sapiens
31 KAAG1  
Affinity Capture-MS Homo sapiens
32 Sart1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which MAN2C1 is involved
PathwayEvidenceSource
Lysosomal oligosaccharide catabolism TAS Reactome
Metabolism TAS Reactome
Metabolism of carbohydrates TAS Reactome





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