Gene description for PHLDA3
Gene name pleckstrin homology-like domain, family A, member 3
Gene symbol PHLDA3
Other names/aliases TIH1
Species Homo sapiens
 Database cross references - PHLDA3
ExoCarta ExoCarta_23612
Vesiclepedia VP_23612
Entrez Gene 23612
HGNC 8934
MIM 607054
UniProt Q9Y5J5  
 PHLDA3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for PHLDA3
Molecular Function
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 IDA
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 IDA
    phosphatidylinositol-5-phosphate binding GO:0010314 IDA
    phosphatidylinositol-3-phosphate binding GO:0032266 IDA
    phosphatidylinositol-3,4-bisphosphate binding GO:0043325 IDA
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 IDA
Biological Process
    anatomical structure morphogenesis GO:0009653 TAS
    intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:0042771 IBA
    intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:0042771 IDA
    positive regulation of apoptotic process GO:0043065 IBA
    positive regulation of apoptotic process GO:0043065 IDA
    negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051898 IBA
    negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051898 IDA
Subcellular Localization
    cytoplasm GO:0005737 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified PHLDA3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PHLDA3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SSR4 6748
Affinity Capture-MS Homo sapiens
2 AHSA1 10598
Affinity Capture-MS Homo sapiens
3 SNX27 81609
Affinity Capture-MS Homo sapiens
4 RARS2  
Affinity Capture-MS Homo sapiens
5 NUP205 23165
Affinity Capture-MS Homo sapiens
6 SLC25A12 8604
Affinity Capture-MS Homo sapiens
7 HNRNPM 4670
Affinity Capture-MS Homo sapiens
8 ZW10 9183
Affinity Capture-MS Homo sapiens
9 TTC27 55622
Affinity Capture-MS Homo sapiens
10 HSPA8 3312
Affinity Capture-MS Homo sapiens
11 PDCD6IP 10015
Affinity Capture-MS Homo sapiens
12 APEX1 328
Affinity Capture-RNA Homo sapiens
13 DNAAF5 54919
Affinity Capture-MS Homo sapiens
14 TNPO3 23534
Affinity Capture-MS Homo sapiens
15 FBXO44  
Affinity Capture-MS Homo sapiens
16 APP 351
Reconstituted Complex Homo sapiens
17 XPO4 64328
Affinity Capture-MS Homo sapiens
18 ATP5L 10632
Affinity Capture-MS Homo sapiens
19 LRPPRC 10128
Affinity Capture-MS Homo sapiens
20 EFTUD2 9343
Affinity Capture-MS Homo sapiens
21 SMAP1 60682
Affinity Capture-MS Homo sapiens
22 CDK2 1017
Two-hybrid Homo sapiens
23 PDXDC1 23042
Affinity Capture-MS Homo sapiens
24 XPO5 57510
Affinity Capture-MS Homo sapiens
25 MMS19 64210
Affinity Capture-MS Homo sapiens
26 IPO4 79711
Affinity Capture-MS Homo sapiens
27 NUP93 9688
Affinity Capture-MS Homo sapiens
28 CCDC47 57003
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PHLDA3 is involved
No pathways found





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