Gene description for Top2a
Gene name topoisomerase (DNA) II alpha
Gene symbol Top2a
Other names/aliases Top-2
Species Mus musculus
 Database cross references - Top2a
ExoCarta ExoCarta_21973
Vesiclepedia VP_21973
Entrez Gene 21973
UniProt Q01320  
 Top2a identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Top2a
Molecular Function
    magnesium ion binding GO:0000287 ISO
    magnesium ion binding GO:0000287 ISS
    DNA binding GO:0003677 ISO
    chromatin binding GO:0003682 IEA
    chromatin binding GO:0003682 ISO
    DNA topoisomerase activity GO:0003916 ISO
    DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity GO:0003918 IDA
    DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity GO:0003918 IGI
    DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity GO:0003918 ISO
    protein kinase C binding GO:0005080 IEA
    protein kinase C binding GO:0005080 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    ATP-dependent activity, acting on DNA GO:0008094 ISO
    DNA binding, bending GO:0008301 ISO
    DNA binding, bending GO:0008301 ISS
    DNA negative supercoiling activity GO:0034335 IEA
    protein homodimerization activity GO:0042803 IEA
    protein homodimerization activity GO:0042803 ISO
    ubiquitin binding GO:0043130 IEA
    ubiquitin binding GO:0043130 ISO
    sequence-specific DNA binding GO:0043565 ISO
    protein heterodimerization activity GO:0046982 IEA
    protein heterodimerization activity GO:0046982 ISO
Biological Process
    resolution of meiotic recombination intermediates GO:0000712 IBA
    sister chromatid segregation GO:0000819 IBA
    hematopoietic progenitor cell differentiation GO:0002244 IMP
    DNA topological change GO:0006265 IDA
    DNA topological change GO:0006265 ISO
    DNA ligation GO:0006266 IEA
    DNA ligation GO:0006266 ISO
    DNA damage response GO:0006974 IEA
    DNA damage response GO:0006974 ISO
    chromosome segregation GO:0007059 IDA
    chromosome segregation GO:0007059 IMP
    chromosome segregation GO:0007059 ISO
    female meiotic nuclear division GO:0007143 IMP
    chromosome condensation GO:0030261 IMP
    apoptotic chromosome condensation GO:0030263 IBA
    apoptotic chromosome condensation GO:0030263 IEA
    apoptotic chromosome condensation GO:0030263 ISO
    embryonic cleavage GO:0040016 IMP
    regulation of circadian rhythm GO:0042752 IMP
    positive regulation of apoptotic process GO:0043065 IEA
    positive regulation of apoptotic process GO:0043065 ISO
    positive regulation of single stranded viral RNA replication via double stranded DNA intermediate GO:0045870 IEA
    positive regulation of single stranded viral RNA replication via double stranded DNA intermediate GO:0045870 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    rhythmic process GO:0048511 IEA
    female meiosis chromosome separation GO:0051309 TAS
    negative regulation of DNA duplex unwinding GO:1905463 ISO
    negative regulation of DNA duplex unwinding GO:1905463 ISS
Subcellular Localization
    nuclear chromosome GO:0000228 IEA
    nuclear chromosome GO:0000228 ISO
    chromosome, centromeric region GO:0000775 IDA
    condensed chromosome GO:0000793 IDA
    condensed chromosome GO:0000793 IMP
    male germ cell nucleus GO:0001673 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    nucleoplasm GO:0005654 ISS
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IDA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    centriole GO:0005814 IEA
    centriole GO:0005814 ISO
    DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex GO:0009330 IEA
    DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex GO:0009330 ISO
    protein-containing complex GO:0032991 ISO
    ribonucleoprotein complex GO:1990904 ISO
    ribonucleoprotein complex GO:1990904 ISS
 Experiment description of studies that identified Top2a in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Top2a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Vezf1  
Affinity Capture-MS Mus musculus
2 Lmna 16905
Affinity Capture-MS Mus musculus
3 Ercc6l  
Co-fractionation Mus musculus
4 Pou5f1  
Affinity Capture-MS Mus musculus
5 Prim1  
Co-fractionation Mus musculus
6 MAPK1 5594
Reconstituted Complex Homo sapiens
7 Prim2  
Co-fractionation Mus musculus
8 Cebpa  
Affinity Capture-MS Mus musculus
9 Cul3  
Affinity Capture-MS Mus musculus
10 Atg5  
Affinity Capture-MS Mus musculus
11 Zbtb16  
Affinity Capture-MS Mus musculus
12 Pole  
Co-fractionation Mus musculus
13 Unk  
Affinity Capture-RNA Mus musculus
14 Nanog  
Affinity Capture-MS Mus musculus
15 Parp1 11545
Co-fractionation Mus musculus
16 Arid1a  
Co-fractionation Mus musculus
17 Mapk1 26413
Affinity Capture-Western Mus musculus
18 Pole2  
Co-fractionation Mus musculus
19 Top1 21969
Co-fractionation Mus musculus
20 Atxn1  
Affinity Capture-MS Mus musculus
21 Atg16l1  
Affinity Capture-MS Mus musculus
22 Uhrf1  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
Co-localization Mus musculus
23 Gsg2  
Co-fractionation Mus musculus
24 Fancd2  
Affinity Capture-MS Mus musculus
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