Gene description for Hspa8
Gene name heat shock protein 8
Gene symbol Hspa8
Other names/aliases 2410008N15Rik
Hsc70
Hsc71
Hsc73
Hsp73
Hspa10
Species Mus musculus
 Database cross references - Hspa8
ExoCarta ExoCarta_15481
Vesiclepedia VP_15481
Entrez Gene 15481
UniProt P63017  
 Hspa8 identified in sEVs derived from the following tissue/cell type
B cells 12626558    
Basophilic leukemia cells 20424270    
Brain cancer cells 19109410    
Colon cancer cells 37309723    
Dendritic cells 10545503    
Embryonic fibroblasts 18494037    
Fibroblasts 23260141    
Hippocampal neuronal cells 30967557    
Hippocampal neuronal cells 30967557    
Macrophages 12626558    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 12626558    
Mast cells 17486113    
Microglia 16081791    
Mov neuroglial cells 15210972    
Oligodendrocytes 21136642    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Hspa8
Molecular Function
    G protein-coupled receptor binding GO:0001664 ISO
    phosphatidylserine binding GO:0001786 IDA
    RNA binding GO:0003723 IEA
    RNA binding GO:0003723 ISO
    signaling receptor binding GO:0005102 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    ATP hydrolysis activity GO:0016887 IBA
    ATP hydrolysis activity GO:0016887 IDA
    ATP hydrolysis activity GO:0016887 ISO
    enzyme binding GO:0019899 IEA
    enzyme binding GO:0019899 ISO
    protein-macromolecule adaptor activity GO:0030674 ISO
    protein-macromolecule adaptor activity GO:0030674 ISS
    heat shock protein binding GO:0031072 IBA
    heat shock protein binding GO:0031072 ISO
    ubiquitin protein ligase binding GO:0031625 ISO
    A1 adenosine receptor binding GO:0031686 IEA
    A1 adenosine receptor binding GO:0031686 ISO
    peptide binding GO:0042277 IEA
    peptide binding GO:0042277 ISO
    ADP binding GO:0043531 IEA
    ADP binding GO:0043531 ISO
    protein folding chaperone GO:0044183 IBA
    unfolded protein binding GO:0051082 IPI
    unfolded protein binding GO:0051082 ISO
    protein-folding chaperone binding GO:0051087 ISO
    C3HC4-type RING finger domain binding GO:0055131 ISO
    ATP-dependent protein disaggregase activity GO:0140545 ISO
    ATP-dependent protein folding chaperone GO:0140662 IEA
    prostaglandin binding GO:1904593 IEA
    prostaglandin binding GO:1904593 ISO
    clathrin-uncoating ATPase activity GO:1990833 IEA
    clathrin-uncoating ATPase activity GO:1990833 ISO
Biological Process
    G1/S transition of mitotic cell cycle GO:0000082 IEA
    mRNA splicing, via spliceosome GO:0000398 NAS
    kidney development GO:0001822 IEA
    positive regulation of T cell mediated cytotoxicity GO:0001916 IEA
    protein folding GO:0006457 IDA
    protein import into nucleus GO:0006606 IEA
    skeletal muscle tissue development GO:0007519 IEA
    response to xenobiotic stimulus GO:0009410 IEA
    response to nickel cation GO:0010045 IEA
    negative regulation of cardiac muscle cell apoptotic process GO:0010667 IEA
    response to activity GO:0014823 IEA
    cerebellum development GO:0021549 IEA
    forebrain development GO:0030900 IEA
    regulation of protein stability GO:0031647 ISO
    response to estradiol GO:0032355 IEA
    response to progesterone GO:0032570 IEA
    protein-containing complex disassembly GO:0032984 ISO
    cellular response to heat GO:0034605 IEA
    protein refolding GO:0042026 IBA
    protein refolding GO:0042026 ISO
    protein refolding GO:0042026 ISS
    response to starvation GO:0042594 IEA
    protein transmembrane import into intracellular organelle GO:0044743 IEA
    modulation by host of viral process GO:0044788 IMP
    positive regulation by host of viral genome replication GO:0044829 IMP
    estrous cycle GO:0044849 IEA
    response to ethanol GO:0045471 IEA
    positive regulation of proteolysis GO:0045862 IEA
    negative regulation of DNA-templated transcription GO:0045892 ISO
    negative regulation of DNA-templated transcription GO:0045892 ISS
    ATP metabolic process GO:0046034 ISO
    positive regulation of mRNA splicing, via spliceosome GO:0048026 IMP
    positive regulation of phagocytosis GO:0050766 IEA
    chaperone cofactor-dependent protein refolding GO:0051085 IBA
    chaperone cofactor-dependent protein refolding GO:0051085 IGI
    regulation of cell cycle GO:0051726 IDA
    chaperone-mediated protein folding GO:0061077 IDA
    chaperone-mediated protein folding GO:0061077 IEP
    chaperone-mediated protein folding GO:0061077 IMP
    regulation of protein complex stability GO:0061635 ISS
    chaperone-mediated autophagy GO:0061684 ISO
    chaperone-mediated autophagy GO:0061684 ISS
    late endosomal microautophagy GO:0061738 IMP
    protein targeting to lysosome involved in chaperone-mediated autophagy GO:0061740 ISO
    protein targeting to lysosome involved in chaperone-mediated autophagy GO:0061740 ISS
    cellular response to hydrogen peroxide GO:0070301 IEA
    cellular response to cadmium ion GO:0071276 IEA
    clathrin coat disassembly GO:0072318 IBA
    clathrin coat disassembly GO:0072318 IDA
    clathrin coat disassembly GO:0072318 IGI
    clathrin coat disassembly GO:0072318 ISO
    positive regulation of lysosomal membrane permeability GO:0097214 IEA
    maintenance of postsynaptic specialization structure GO:0098880 IEA
    regulation of postsynapse organization GO:0099175 EXP
    regulation of postsynapse organization GO:0099175 IDA
    negative regulation of NLRP3 inflammasome complex assembly GO:1900226 ISO
    negative regulation of supramolecular fiber organization GO:1902904 ISO
    positive regulation of protein refolding GO:1904592 IEA
    chaperone-mediated autophagy translocation complex disassembly GO:1904764 ISS
    slow axonal transport GO:1990832 IEA
    response to odorant GO:1990834 IEA
Subcellular Localization
    Prp19 complex GO:0000974 ISO
    Prp19 complex GO:0000974 ISS
    photoreceptor inner segment GO:0001917 IEA
    photoreceptor inner segment GO:0001917 ISO
    intracellular anatomical structure GO:0005622 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    spliceosomal complex GO:0005681 ISO
    nucleolus GO:0005730 IEA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    lysosomal membrane GO:0005765 ISO
    lysosomal membrane GO:0005765 ISO
    lysosomal membrane GO:0005765 ISS
    lysosomal membrane GO:0005765 ISS
    late endosome GO:0005770 IDA
    autophagosome GO:0005776 IEA
    autophagosome GO:0005776 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    microtubule GO:0005874 IEA
    microtubule GO:0005874 ISO
    intermediate filament GO:0005882 IEA
    intermediate filament GO:0005882 ISO
    plasma membrane GO:0005886 IBA
    synaptic vesicle GO:0008021 IEA
    synaptic vesicle GO:0008021 ISO
    cell surface GO:0009986 IEA
    cell surface GO:0009986 ISO
    postsynaptic density GO:0014069 IEA
    postsynaptic density GO:0014069 ISO
    postsynaptic density GO:0014069 ISO
    axon GO:0030424 ISO
    dendrite GO:0030425 ISO
    late endosome lumen GO:0031906 TAS
    asymmetric synapse GO:0032279 ISO
    protein-containing complex GO:0032991 ISO
    melanosome GO:0042470 IEA
    neuronal cell body GO:0043025 ISO
    terminal bouton GO:0043195 IEA
    terminal bouton GO:0043195 ISO
    dendritic spine GO:0043197 IEA
    dendritic spine GO:0043197 ISO
    dendritic shaft GO:0043198 IEA
    dendritic shaft GO:0043198 ISO
    perikaryon GO:0043204 IEA
    perikaryon GO:0043204 ISO
    myelin sheath GO:0043209 HDA
    neuron spine GO:0044309 ISO
    perinuclear region of cytoplasm GO:0048471 IDA
    perinuclear region of cytoplasm GO:0048471 ISO
    extracellular exosome GO:0070062 IDA
    extracellular exosome GO:0070062 ISO
    photoreceptor ribbon synapse GO:0098684 IDA
    glycinergic synapse GO:0098690 IDA
    glycinergic synapse GO:0098690 ISO
    presynapse GO:0098793 IDA
    presynapse GO:0098793 IEP
    presynapse GO:0098793 IMP
    presynapse GO:0098793 ISO
    postsynapse GO:0098794 ISO
    glutamatergic synapse GO:0098978 EXP
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 ISO
    presynaptic cytosol GO:0099523 ISO
    postsynaptic cytosol GO:0099524 ISO
    postsynaptic specialization membrane GO:0099634 IDA
    protein folding chaperone complex GO:0101031 ISO
    lysosomal matrix GO:1990836 ISO
    ribonucleoprotein complex GO:1990904 ISO
    ribonucleoprotein complex GO:1990904 ISS
 Experiment description of studies that identified Hspa8 in sEVs
1
Experiment ID 8
MISEV standards
✘ Biophysical techniques
✔
HSP60|HSC70|MHCII|CD80|CD86
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 12626558    
Organism Mus musculus
Experiment description Mast cell-derived exosomes induce phenotypic and functional maturation of dendritic cells and elicit specific immune responses in vivo.
Authors "Skokos D, Botros HG, Demeure C, Morin J, Peronet R, Birkenmeier G, Boudaly S, Mecheri S."
Journal name JIMMU
Publication year 2003
Sample B cells
Sample name B cells
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
2
Experiment ID 89
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20424270    
Organism Rattus norvegicus
Experiment description Exosomes account for vesicle-mediated transcellular transport of activatable phospholipases and prostaglandins.
Authors "Subra C, Grand D, Laulagnier K, Stella A, Lambeau G, Paillasse M, De Medina P, Monsarrat B, Perret B, Silvente-Poirot S, Poirot M, Record M."
Journal name JLR
Publication year 2010
Sample Basophilic leukemia cells
Sample name RBL-2H3
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Gas chromatography
Thin layer chromatography
Mass spectrometry [Qstar XL]
FACS
3
Experiment ID 89
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20424270    
Organism Rattus norvegicus
Experiment description Exosomes account for vesicle-mediated transcellular transport of activatable phospholipases and prostaglandins.
Authors "Subra C, Grand D, Laulagnier K, Stella A, Lambeau G, Paillasse M, De Medina P, Monsarrat B, Perret B, Silvente-Poirot S, Poirot M, Record M."
Journal name JLR
Publication year 2010
Sample Basophilic leukemia cells
Sample name RBL-2H3
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Gas chromatography
Thin layer chromatography
Mass spectrometry [Qstar XL]
FACS
4
Experiment ID 26
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|GAPDH|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19109410    
Organism Mus musculus
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors "Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD"
Journal name FASEB
Publication year 2008
Sample Brain cancer cells
Sample name SMA560vIII
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
5
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 3
MISEV standards
✔
IEM
Biophysical techniques
✔
HSP84|HSC73|MHCII|CD9|mac-1
Enriched markers
✔
CANX|HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 10545503    
Organism Mus musculus
Experiment description Molecular characterization of dendritic cell-derived exosomes. Selective accumulation of the heat shock protein hsc73.
Authors "Thery C, Regnault A, Garin J, Wolfers J, Zitvogel L, Ricciardi-Castagnoli P, Raposo G, Amigorena S."
Journal name JCB
Publication year 1999
Sample Dendritic cells
Sample name D1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
7
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
8
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 1065
MISEV standards
✘ Biophysical techniques
✔
Tsg101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Hippocampal neuronal cells
Sample name HT22
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
10
Experiment ID 1066
MISEV standards
✘ Biophysical techniques
✔
Tsg101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Hippocampal neuronal cells
Sample name HT22
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
11
Experiment ID 9
MISEV standards
✘ Biophysical techniques
✔
HSP60|HSC70|MHCII|CD80|CD87
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 12626558    
Organism Mus musculus
Experiment description Mast cell-derived exosomes induce phenotypic and functional maturation of dendritic cells and elicit specific immune responses in vivo.
Authors "Skokos D, Botros HG, Demeure C, Morin J, Peronet R, Birkenmeier G, Boudaly S, Mecheri S."
Journal name JIMMU
Publication year 2003
Sample Macrophages
Sample name Macrophages
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
12
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
14
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
15
Experiment ID 7
MISEV standards
✘ Biophysical techniques
✔
HSP60|HSC70|MHCII|CD80|CD86
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 12626558    
Organism Mus musculus
Experiment description Mast cell-derived exosomes induce phenotypic and functional maturation of dendritic cells and elicit specific immune responses in vivo.
Authors "Skokos D, Botros HG, Demeure C, Morin J, Peronet R, Birkenmeier G, Boudaly S, Mecheri S."
Journal name JIMMU
Publication year 2003
Sample Mast cells
Sample name Bone marrow-derived mast cells
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
16
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
17
Experiment ID 14
MISEV standards
✔
EM
Biophysical techniques
✔
RAB7|RAB11|CD9|CD63|LAMP1|LAMP2
Enriched markers
✔
DNM
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors "Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L"
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
18
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
19
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
20
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
21
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hspa8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Nphp3  
Affinity Capture-MS Mus musculus
2 Cep290  
Affinity Capture-MS Mus musculus
3 Kcna5  
Affinity Capture-Western Mus musculus
4 Cbx1  
Affinity Capture-MS Mus musculus
5 Fmr1  
Co-fractionation Mus musculus
6 Dnajc5 13002
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
7 Usp25 30940
Affinity Capture-MS Mus musculus
8 Tmem173  
Proximity Label-MS Mus musculus
9 Tubgcp2  
Co-fractionation Mus musculus
10 Hspa8 15481
Reconstituted Complex Mus musculus
Reconstituted Complex Mus musculus
11 HIST1H3A 8350
Affinity Capture-MS Homo sapiens
12 SOD1 6647
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
13 Ube2v1 66589
Co-fractionation Mus musculus
14 Inadl  
Affinity Capture-MS Mus musculus
15 Htt  
Affinity Capture-Western Mus musculus
Co-localization Mus musculus
16 Kcnma1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
17 Stip1 20867
Co-fractionation Mus musculus
18 Sf3b1 81898
Co-fractionation Mus musculus
19 Sugt1 67955
Co-fractionation Mus musculus
20 Tsc1  
Affinity Capture-MS Mus musculus
21 Erg  
Affinity Capture-Western Mus musculus
22 Ahi1  
Affinity Capture-MS Mus musculus
23 Atxn1  
Proximity Label-MS Mus musculus
24 Sfpq 71514
Co-fractionation Mus musculus
25 Kctd13  
Affinity Capture-MS Mus musculus
26 Kdm2b  
Affinity Capture-MS Mus musculus
27 Tfe3  
Affinity Capture-MS Mus musculus
28 Rbbp7 245688
Co-fractionation Mus musculus
29 MTNR1A  
Reconstituted Complex Homo sapiens
30 Snap25 20614
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
31 Sgta 52551
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
32 Bag3  
Affinity Capture-Western Mus musculus
33 Mks1  
Affinity Capture-MS Mus musculus
34 Iqcb1  
Affinity Capture-MS Mus musculus
35 Ago1 236511
Co-fractionation Mus musculus
36 Ywhae 22627
Affinity Capture-MS Mus musculus
37 Stub1 56424
Co-fractionation Mus musculus
38 Myd88  
Affinity Capture-MS Mus musculus
39 Atxn3  
Affinity Capture-Western Mus musculus
40 Nphp4  
Affinity Capture-MS Mus musculus
41 Fzr1  
Affinity Capture-MS Mus musculus
42 Map3k14  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
43 Traf6  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
44 MAPT  
Reconstituted Complex Homo sapiens
45 Cidec  
Affinity Capture-MS Mus musculus
46 Zfand2a  
Affinity Capture-MS Mus musculus
47 Skp1a 21402
Co-fractionation Mus musculus
48 Syn2  
Co-fractionation Mus musculus
49 Chaf1a  
Affinity Capture-MS Mus musculus
50 Sra1  
Co-fractionation Mus musculus
51 Hspb2 69253
Affinity Capture-MS Mus musculus
52 Twist1  
Proximity Label-MS Mus musculus
53 Grin1  
Affinity Capture-MS Mus musculus
54 Rps3a1 20091
Co-fractionation Mus musculus
55 Cbx3 12417
Affinity Capture-MS Mus musculus
56 HACE1 57531
Affinity Capture-MS Homo sapiens
57 Invs  
Affinity Capture-MS Mus musculus
58 Rnf2  
Affinity Capture-MS Mus musculus
59 Eif4g1 208643
Co-fractionation Mus musculus
60 Rbbp4 19646
Co-fractionation Mus musculus
61 Rock2  
Co-fractionation Mus musculus
62 Rps9 76846
Co-fractionation Mus musculus
63 Vcp 269523
Co-fractionation Mus musculus
64 Eed  
Affinity Capture-MS Mus musculus
65 Syncrip 56403
Co-fractionation Mus musculus
66 Mapt  
Affinity Capture-Western Mus musculus
67 PRKACB  
Affinity Capture-MS Bos taurus
68 Nphp1  
Affinity Capture-MS Mus musculus
69 Srrt 83701
Co-fractionation Mus musculus
70 BAG4  
Two-hybrid Homo sapiens
71 Fancd2  
Affinity Capture-MS Mus musculus
72 Lipc  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Hspa8 is involved
PathwayEvidenceSource
Antiviral mechanism by IFN-stimulated genes IEA Reactome
Attenuation phase IEA Reactome
AUF1 (hnRNP D0) binds and destabilizes mRNA IEA Reactome
Cellular response to heat stress IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Clathrin-mediated endocytosis IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
GABA synthesis, release, reuptake and degradation IEA Reactome
Golgi Associated Vesicle Biogenesis IEA Reactome
HSF1-dependent transactivation IEA Reactome
HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
Interferon Signaling IEA Reactome
Lysosome Vesicle Biogenesis IEA Reactome
Membrane Trafficking IEA Reactome
Metabolism of proteins IEA Reactome
Metabolism of RNA IEA Reactome
mRNA Splicing IEA Reactome
mRNA Splicing - Major Pathway IEA Reactome
Neuronal System IEA Reactome
Neurotransmitter release cycle IEA Reactome
Neutrophil degranulation IEA Reactome
PKR-mediated signaling IEA Reactome
Post-translational protein modification IEA Reactome
Processing of Capped Intron-Containing Pre-mRNA IEA Reactome
Protein methylation IEA Reactome
Regulation of HSF1-mediated heat shock response IEA Reactome
Regulation of mRNA stability by proteins that bind AU-rich elements IEA Reactome
trans-Golgi Network Vesicle Budding IEA Reactome
Transmission across Chemical Synapses IEA Reactome
Vesicle-mediated transport IEA Reactome





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