Gene description for Rbbp4
Gene name retinoblastoma binding protein 4
Gene symbol Rbbp4
Other names/aliases RBAP48
mRbAp48
Species Mus musculus
 Database cross references - Rbbp4
ExoCarta ExoCarta_19646
Vesiclepedia VP_19646
Entrez Gene 19646
UniProt Q60972  
 Rbbp4 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rbbp4
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IDA
    protein binding GO:0005515 IPI
    ATP-dependent activity, acting on DNA GO:0008094 ISO
    ATP-dependent activity, acting on DNA GO:0008094 ISS
    histone binding GO:0042393 IBA
    histone binding GO:0042393 IEA
    histone binding GO:0042393 ISO
    histone deacetylase binding GO:0042826 IEA
    histone deacetylase binding GO:0042826 ISO
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 NAS
    DNA replication GO:0006260 IEA
    nucleosome assembly GO:0006334 IEA
    DNA replication-dependent chromatin assembly GO:0006335 ISO
    DNA replication-dependent chromatin assembly GO:0006335 ISS
    chromatin remodeling GO:0006338 IBA
    chromatin remodeling GO:0006338 ISO
    chromatin remodeling GO:0006338 ISS
    chromatin remodeling GO:0006338 TAS
    regulation of DNA-templated transcription GO:0006355 IBA
    regulation of DNA-templated transcription GO:0006355 ISO
    brain development GO:0007420 NAS
    negative regulation of cell migration GO:0030336 NAS
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 NAS
    regulation of cell fate specification GO:0042659 NAS
    negative regulation of DNA-templated transcription GO:0045892 NAS
    positive regulation of DNA-templated transcription GO:0045893 NAS
    negative regulation of stem cell population maintenance GO:1902455 NAS
    positive regulation of stem cell population maintenance GO:1902459 NAS
    regulation of stem cell differentiation GO:2000736 NAS
Subcellular Localization
    histone deacetylase complex GO:0000118 ISO
    chromosome, telomeric region GO:0000781 IEA
    chromosome, telomeric region GO:0000781 ISO
    chromatin GO:0000785 ISO
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 ISO
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    NuRD complex GO:0016581 IBA
    NuRD complex GO:0016581 IDA
    NuRD complex GO:0016581 ISO
    NuRD complex GO:0016581 NAS
    NURF complex GO:0016589 ISO
    protein-containing complex GO:0032991 ISO
    CAF-1 complex GO:0033186 ISO
    CAF-1 complex GO:0033186 ISS
    ESC/E(Z) complex GO:0035098 IBA
    ESC/E(Z) complex GO:0035098 IDA
    ESC/E(Z) complex GO:0035098 ISO
    Sin3-type complex GO:0070822 NAS
    ATPase complex GO:1904949 ISO
 Experiment description of studies that identified Rbbp4 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rbbp4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Jarid2  
Affinity Capture-MS Mus musculus
2 Esrrb  
Affinity Capture-MS Mus musculus
3 Hspa8 15481
Co-fractionation Mus musculus
4 HIST1H3A 8350
Affinity Capture-MS Homo sapiens
5 Hcfc1  
Co-fractionation Mus musculus
6 Cbx5  
Affinity Capture-MS Mus musculus
7 Kdm2b  
Affinity Capture-MS Mus musculus
8 Gata1  
Affinity Capture-Western Mus musculus
9 Atxn1  
Affinity Capture-MS Mus musculus
10 Runx1 12394
Affinity Capture-MS Mus musculus
11 Tfe3  
Affinity Capture-MS Mus musculus
12 Rbbp7 245688
Co-fractionation Mus musculus
13 Suz12  
Affinity Capture-MS Mus musculus
14 Chaf1a  
Affinity Capture-MS Mus musculus
15 Mecp2  
Affinity Capture-MS Mus musculus
16 Ezh2  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
17 Hdac1 433759
Affinity Capture-Western Mus musculus
18 Mettl21e  
Affinity Capture-MS Mus musculus
19 Mtf2  
Affinity Capture-MS Mus musculus
20 Ezh1  
Affinity Capture-MS Mus musculus
21 Ran 19384
Co-fractionation Mus musculus
22 Nanog  
Affinity Capture-MS Mus musculus
23 Rnf2  
Affinity Capture-MS Mus musculus
24 Eftud2 20624
Co-fractionation Mus musculus
25 Eed  
Affinity Capture-MS Mus musculus
26 Atg16l1  
Affinity Capture-MS Mus musculus
27 Sall4  
Affinity Capture-MS Mus musculus
28 Otx2  
Affinity Capture-MS Mus musculus
29 Mta2 23942
Affinity Capture-Western Mus musculus
30 Fancd2  
Affinity Capture-MS Mus musculus
31 Tcf3  
Affinity Capture-MS Mus musculus
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