Gene description for Dnajc5
Gene name DnaJ (Hsp40) homolog, subfamily C, member 5
Gene symbol Dnajc5
Other names/aliases 2610314I24Rik
AU018536
Csp
Species Mus musculus
 Database cross references - Dnajc5
ExoCarta ExoCarta_13002
Vesiclepedia VP_13002
Entrez Gene 13002
UniProt P60904  
 Dnajc5 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Dnajc5
Molecular Function
    protein binding GO:0005515 IPI
    ATP-dependent protein binding GO:0043008 IEA
    ATP-dependent protein binding GO:0043008 ISO
Biological Process
    negative regulation of neuron apoptotic process GO:0043524 IGI
    neuron apoptotic process GO:0051402 IGI
    chaperone-mediated protein folding GO:0061077 IBA
    chaperone-mediated protein folding GO:0061077 IDA
    chaperone-mediated protein folding GO:0061077 IEP
    chaperone-mediated protein folding GO:0061077 IMP
    regulation of synaptic vesicle cycle GO:0098693 IBA
    regulation of synaptic vesicle cycle GO:0098693 IDA
    regulation of synaptic vesicle cycle GO:0098693 IEP
    regulation of synaptic vesicle cycle GO:0098693 IMP
Subcellular Localization
    Golgi apparatus GO:0005794 IEA
    Golgi apparatus GO:0005794 ISO
    cytosol GO:0005829 ISS
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    synaptic vesicle GO:0008021 IDA
    membrane GO:0016020 IBA
    membrane GO:0016020 ISS
    synaptic vesicle membrane GO:0030672 IEA
    synaptic vesicle membrane GO:0030672 ISO
    neuromuscular junction GO:0031594 IDA
    neuromuscular junction GO:0031594 IEP
    neuromuscular junction GO:0031594 IMP
    melanosome GO:0042470 IEA
    chromaffin granule membrane GO:0042584 IEA
    intracellular membrane-bounded organelle GO:0043231 IBA
    intracellular membrane-bounded organelle GO:0043231 ISO
    clathrin-sculpted glutamate transport vesicle membrane GO:0060203 TAS
    presynapse GO:0098793 IBA
    presynapse GO:0098793 IDA
    presynapse GO:0098793 IEP
    presynapse GO:0098793 IMP
 Experiment description of studies that identified Dnajc5 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dnajc5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Hspa8 15481
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
2 Dnajc5 13002
Reconstituted Complex Mus musculus
Reconstituted Complex Mus musculus
3 Snap25 20614
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
4 Sgta 52551
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
5 SNCA 6622
Phenotypic Suppression Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here