Gene description for CLCN3
Gene name chloride channel, voltage-sensitive 3
Gene symbol CLCN3
Other names/aliases CLC3
ClC-3
Species Homo sapiens
 Database cross references - CLCN3
ExoCarta ExoCarta_1182
Vesiclepedia VP_1182
Entrez Gene 1182
HGNC 2021
MIM 600580
UniProt P51790  
 CLCN3 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for CLCN3
Molecular Function
    voltage-gated chloride channel activity GO:0005247 IBA
    chloride channel activity GO:0005254 IDA
    chloride channel activity GO:0005254 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    antiporter activity GO:0015297 ISS
    PDZ domain binding GO:0030165 IDA
    volume-sensitive chloride channel activity GO:0072320 IMP
Biological Process
    regulation of pH GO:0006885 TAS
    phagocytosis, engulfment GO:0006911 IEA
    adult locomotory behavior GO:0008344 IEA
    synaptic transmission, glutamatergic GO:0035249 IEA
    photoreceptor cell maintenance GO:0045494 IEA
    negative regulation of cell volume GO:0045794 IMP
    endosomal lumen acidification GO:0048388 TAS
    synaptic transmission, GABAergic GO:0051932 IEA
    synaptic vesicle lumen acidification GO:0097401 IEA
    chloride transmembrane transport GO:1902476 IDA
    positive regulation of reactive oxygen species biosynthetic process GO:1903428 IEA
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    lysosomal membrane GO:0005765 ISS
    early endosome GO:0005769 IBA
    early endosome GO:0005769 IDA
    late endosome GO:0005770 IDA
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    synaptic vesicle GO:0008021 IBA
    synaptic vesicle GO:0008021 IDA
    external side of plasma membrane GO:0009897 IDA
    cell surface GO:0009986 NAS
    endosome membrane GO:0010008 ISS
    vesicle membrane GO:0012506 IDA
    membrane GO:0016020 HDA
    membrane GO:0016020 TAS
    secretory granule GO:0030141 IDA
    synaptic vesicle membrane GO:0030672 IDA
    synaptic vesicle membrane GO:0030672 IMP
    cytoplasmic vesicle GO:0031410 IDA
    early endosome membrane GO:0031901 IEA
    late endosome membrane GO:0031902 IEA
    ruffle membrane GO:0032587 IEA
    specific granule GO:0042581 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    axon terminus GO:0043679 IEA
    phagocytic vesicle GO:0045335 IDA
    recycling endosome GO:0055037 ISS
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    GABA-ergic synapse GO:0098982 IDA
    GABA-ergic synapse GO:0098982 IMP
 Experiment description of studies that identified CLCN3 in exosomes
1
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CLCN3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CYP26B1  
Affinity Capture-MS Homo sapiens
2 LGALS9 3965
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 LYPD3 27076
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 CNGA4  
Affinity Capture-MS Homo sapiens
5 ICOSLG  
Affinity Capture-MS Homo sapiens
6 TMEM9 252839
Affinity Capture-MS Homo sapiens
7 FEM1A  
Affinity Capture-MS Homo sapiens
8 PDZK1 5174
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
9 CIB2  
Affinity Capture-MS Homo sapiens
10 OR10H2  
Affinity Capture-MS Homo sapiens
11 C3orf52  
Affinity Capture-MS Homo sapiens
12 SCAMP1 9522
Affinity Capture-MS Homo sapiens
13 LGALS3 3958
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 CCDC107  
Affinity Capture-MS Homo sapiens
15 MTAP 4507
Cross-Linking-MS (XL-MS) Homo sapiens
16 TMEM9B 56674
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 HSPB1 3315
Two-hybrid Homo sapiens
18 TNFSF12 8742
Affinity Capture-MS Homo sapiens
19 MOGAT2  
Affinity Capture-MS Homo sapiens
20 CLCN3 1182
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 OR4N2  
Affinity Capture-MS Homo sapiens
22 SCAP  
Affinity Capture-MS Homo sapiens
23 APEX1 328
Affinity Capture-RNA Homo sapiens
24 KCNE3  
Affinity Capture-MS Homo sapiens
25 ASB14  
Affinity Capture-MS Homo sapiens
26 LGALS9B 284194
Affinity Capture-MS Homo sapiens
27 ATP2B2 491
Affinity Capture-MS Homo sapiens
28 SLC9A3R1 9368
Reconstituted Complex Homo sapiens
29 CYP19A1  
Affinity Capture-MS Homo sapiens
30 TMEM116  
Affinity Capture-MS Homo sapiens
31 PCDHB7  
Affinity Capture-MS Homo sapiens
32 TPCN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 FAM96A  
Affinity Capture-MS Homo sapiens
34 LGALS8 3964
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 CFTR 1080
Reconstituted Complex Homo sapiens
36 CHRND  
Affinity Capture-MS Homo sapiens
37 PIGH  
Affinity Capture-MS Homo sapiens
38 ACVR2B  
Affinity Capture-MS Homo sapiens
39 CLEC4E  
Affinity Capture-MS Homo sapiens
40 GGA1 26088
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 CLCN5 1184
Affinity Capture-MS Homo sapiens
42 GOPC 57120
Reconstituted Complex Homo sapiens
43 PCDHB3  
Affinity Capture-MS Homo sapiens
44 AVPR2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CLCN3 is involved
PathwayEvidenceSource
Ion channel transport IEA Reactome
Stimuli-sensing channels IEA Reactome
Transport of small molecules IEA Reactome





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