Gene description for BLOC1S4
Gene name biogenesis of lysosomal organelles complex-1, subunit 4, cappuccino
Gene symbol BLOC1S4
Other names/aliases BCAS4L
BLOS4
CNO
Species Homo sapiens
 Database cross references - BLOC1S4
ExoCarta ExoCarta_55330
Vesiclepedia VP_55330
Entrez Gene 55330
HGNC 24206
MIM 605695
UniProt Q9NUP1  
 BLOC1S4 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for BLOC1S4
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    anterograde axonal transport GO:0008089 ISS
    neuron projection development GO:0031175 ISS
    melanosome organization GO:0032438 NAS
    anterograde synaptic vesicle transport GO:0048490 ISS
    neuromuscular process controlling balance GO:0050885 IEA
    platelet aggregation GO:0070527 IEA
Subcellular Localization
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 TAS
    BLOC-1 complex GO:0031083 IBA
    BLOC-1 complex GO:0031083 IDA
    BLOC-1 complex GO:0031083 IPI
    axon cytoplasm GO:1904115 IEA
 Experiment description of studies that identified BLOC1S4 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for BLOC1S4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BRK1 55845
Affinity Capture-MS Homo sapiens
2 POC1B 282809
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 C10orf32  
Affinity Capture-MS Homo sapiens
4 POC1A  
Affinity Capture-MS Homo sapiens
5 CCDC53 51019
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 DTNBP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 BLOC1S5 63915
Affinity Capture-MS Homo sapiens
8 CNGA4  
Affinity Capture-MS Homo sapiens
9 SNAPIN 23557
Two-hybrid Homo sapiens
10 BLOC1S6  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
11 BLOC1S1  
Affinity Capture-MS Homo sapiens
12 BLOC1S2 282991
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
13 EXOC1 55763
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 AIMP2 7965
Two-hybrid Homo sapiens
15 XPO1 7514
Affinity Capture-MS Homo sapiens
16 DDX58 23586
Affinity Capture-RNA Homo sapiens
17 FAM9C  
Affinity Capture-MS Homo sapiens
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