Experiment description of studies that identified GDF10 in sEVs

1 Experiment ID 1333 MISEV standards Biophysical techniques ✔ CD81|EPCAM|CD9 Enriched markers ✘ Negative markers Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35918900     Organism Homo sapiens Experiment description Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL) Authors "Bauzá-Martinez J, Armony G, Pronker MF, Wu W. " Journal name J Extracell Vesicles Publication year 2022 Sample Breast cancer cells Sample name MDA-MB-231 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectometry Western blotting

2 Experiment ID 1333 MISEV standards Biophysical techniques ✔ CD81|EPCAM|CD9 Enriched markers ✘ Negative markers Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35918900     Organism Homo sapiens Experiment description Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL) Authors "Bauzá-Martinez J, Armony G, Pronker MF, Wu W. " Journal name J Extracell Vesicles Publication year 2022 Sample Breast cancer cells Sample name MDA-MB-231 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectometry Western blotting

3 Experiment ID 1333 MISEV standards Biophysical techniques ✔ CD81|EPCAM|CD9 Enriched markers ✘ Negative markers Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35918900     Organism Homo sapiens Experiment description Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL) Authors "Bauzá-Martinez J, Armony G, Pronker MF, Wu W. " Journal name J Extracell Vesicles Publication year 2022 Sample Breast cancer cells Sample name MDA-MB-231 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectometry Western blotting

4 Experiment ID 1334 MISEV standards Biophysical techniques ✔ CD9 Enriched markers ✔ ACTB Negative markers Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35918900     Organism Homo sapiens Experiment description Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL) Authors "Bauzá-Martinez J, Armony G, Pronker MF, Wu W. " Journal name J Extracell Vesicles Publication year 2022 Sample Breast cancer cells Sample name LM2 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectometry

5 Experiment ID 1335 MISEV standards Biophysical techniques ✔ EPCAM|CD9 Enriched markers ✘ Negative markers Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35918900     Organism Homo sapiens Experiment description Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL) Authors "Bauzá-Martinez J, Armony G, Pronker MF, Wu W. " Journal name J Extracell Vesicles Publication year 2022 Sample Breast cancer cells Sample name MDA-MB-231 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectometry

6 Experiment ID 1335 MISEV standards Biophysical techniques ✔ EPCAM|CD9 Enriched markers ✘ Negative markers Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35918900     Organism Homo sapiens Experiment description Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL) Authors "Bauzá-Martinez J, Armony G, Pronker MF, Wu W. " Journal name J Extracell Vesicles Publication year 2022 Sample Breast cancer cells Sample name MDA-MB-231 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectometry

7 Experiment ID 1335 MISEV standards Biophysical techniques ✔ EPCAM|CD9 Enriched markers ✘ Negative markers Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35918900     Organism Homo sapiens Experiment description Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL) Authors "Bauzá-Martinez J, Armony G, Pronker MF, Wu W. " Journal name J Extracell Vesicles Publication year 2022 Sample Breast cancer cells Sample name MDA-MB-231 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectometry

8 Experiment ID 1336 MISEV standards Biophysical techniques ✔ CD9 Enriched markers ✔ ACTB Negative markers Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35918900     Organism Homo sapiens Experiment description Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL) Authors "Bauzá-Martinez J, Armony G, Pronker MF, Wu W. " Journal name J Extracell Vesicles Publication year 2022 Sample Breast cancer cells Sample name LM2 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectometry

Protein-protein interactions for GDF10

Protein Interactor ExoCarta ID Identification method PubMed Species 1 BANP   Affinity Capture-MS Homo sapiens 2 FBXO45 200933 Affinity Capture-MS Homo sapiens 3 MTHFR 4524 Affinity Capture-MS Homo sapiens 4 MYCBP2 23077 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 5 LRP4 4038 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 6 ZNF507   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens View the network

Pathways in which GDF10 is involved

No pathways found