Gene description for Anxa1
Gene name annexin A1
Gene symbol Anxa1
Other names/aliases Anx-1
Anx-A1
C430014K04Rik
Lpc-1
Lpc1
Species Mus musculus
 Database cross references - Anxa1
ExoCarta ExoCarta_16952
Entrez Gene 16952
UniProt P10107  
 Anxa1 identified in exosomes derived from the following tissue/cell type
Dendritic cells 11390481    
Embryonic fibroblasts 18494037    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mov neuroglial cells 15210972    
 Gene ontology annotations for Anxa1
Molecular Function
    phospholipase inhibitor activity GO:0004859 IEA
    protein binding GO:0005515 IPI
    single-stranded DNA binding GO:0003697 ISO
    double-stranded DNA-dependent ATPase activity GO:0033676 ISO
    single-stranded RNA binding GO:0003727 ISO
    annealing helicase activity GO:0036310 ISO
    phospholipase A2 inhibitor activity GO:0019834 ISO
    protein homodimerization activity GO:0042803 ISO
    phospholipid binding GO:0005543 ISO
    calcium-dependent protein binding GO:0048306 ISO
    helicase activity GO:0004386 ISO
    calcium ion binding GO:0005509 IEA
    calcium-dependent phospholipid binding GO:0005544 ISO
    protein binding, bridging GO:0030674 ISO
    structural molecule activity GO:0005198 ISO
Biological Process
    positive regulation of vesicle fusion GO:0031340 ISO
    negative regulation of phospholipase A2 activity GO:1900138 ISO
    peptide cross-linking GO:0018149 ISO
    cellular response to hydrogen peroxide GO:0070301 ISO
    negative regulation of protein secretion GO:0050709 ISO
    cellular response to glucocorticoid stimulus GO:0071385 ISO
    cell surface receptor signaling pathway GO:0007166 ISO
    signal transduction GO:0007165 IMP
    DNA strand renaturation GO:0000733 ISO
    regulation of cell proliferation GO:0042127 IMP
    insulin secretion GO:0030073 ISO
    neutrophil clearance GO:0097350 ISO
    keratinocyte differentiation GO:0030216 ISO
    DNA duplex unwinding GO:0032508 ISO
    neutrophil homeostasis GO:0001780 ISO
    alpha-beta T cell differentiation GO:0046632 IMP
    positive regulation of G1/S transition of mitotic cell cycle GO:1900087 IMP
    prolactin secretion GO:0070459 ISO
    positive regulation of prostaglandin biosynthetic process GO:0031394 ISO
    positive regulation of neutrophil apoptotic process GO:0033031 IMP
    DNA rewinding GO:0036292 ISO
    negative regulation of interleukin-8 secretion GO:2000483 ISO
    positive regulation of apoptotic process GO:0043065 ISO
    arachidonic acid secretion GO:0050482 IMP
Subcellular Localization
    focal adhesion GO:0005925 ISO
    sarcolemma GO:0042383 IDA
    endosome GO:0005768 ISO
    vesicle GO:0031982 ISO
    plasma membrane GO:0005886 ISO
    mitochondrial membrane GO:0031966 ISO
    nucleus GO:0005634 ISO
    protein complex GO:0043234 ISO
    cell projection GO:0042995 IEA
    extracellular exosome GO:0070062 ISO
    membrane GO:0016020 IEA
    cornified envelope GO:0001533 ISO
    mast cell granule GO:0042629 ISO
    cytoplasm GO:0005737 ISO
    extracellular space GO:0005615 ISO
    cell surface GO:0009986 ISO
    cilium GO:0005929 IEA
 Experiment description of studies that identified Anxa1 in exosomes
1
Experiment ID 10
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD9|MHCII|MHCI|CD86|LAMP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
PubMed ID 11390481    
Organism Mus musculus
Experiment description Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles.
Authors Thery C, Boussac M, Veron P, Ricciardi-Castagnoli P, Raposo G, Garin J, Amigorena S.
Journal name JIMMU
Publication year 2001
Sample Dendritic cells
Sample name D1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 73
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 214
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 215
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 216
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 39
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|HSC70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Anxa1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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