Gene description for Lgals3bp
Gene name lectin, galactoside-binding, soluble, 3 binding protein
Gene symbol Lgals3bp
Other names/aliases 90K
CyCAP
MAC-2BP
Ppicap
Tango10b
Species Mus musculus
 Database cross references - Lgals3bp
ExoCarta ExoCarta_19039
Vesiclepedia VP_19039
Entrez Gene 19039
UniProt Q07797  
 Lgals3bp identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Faeces 33431899    
Faeces 33431899    
Fibroblasts 23260141    
Melanoma cells 34957415    
Melanoma cells 34957415    
Melanoma cells 34957415    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Lgals3bp
Molecular Function
    scavenger receptor activity GO:0005044 ISS
    scavenger receptor activity GO:0005044 TAS
    protein binding GO:0005515 IPI
Biological Process
    cell adhesion GO:0007155 IEA
    vesicle-mediated transport GO:0016192 IEA
Subcellular Localization
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 ISO
    membrane GO:0016020 IEA
 Experiment description of studies that identified Lgals3bp in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 303
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
3
Experiment ID 304
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
4
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 1101
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 1102
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
7
Experiment ID 1103
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
8
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Lgals3bp
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PCBP2 5094
Affinity Capture-MS Homo sapiens
2 NUP93 9688
Affinity Capture-MS Homo sapiens
3 Parp14  
Co-fractionation Mus musculus
4 CS 1431
Affinity Capture-MS Homo sapiens
5 OGT 8473
Affinity Capture-MS Homo sapiens
6 TUBGCP2 10844
Affinity Capture-MS Homo sapiens
7 ARHGAP39  
Affinity Capture-MS Homo sapiens
8 MVP 9961
Affinity Capture-MS Homo sapiens
9 MYCBP2 23077
Affinity Capture-MS Homo sapiens
10 H3F3A 3020
Affinity Capture-MS Homo sapiens
11 MYOF 26509
Affinity Capture-MS Homo sapiens
12 MZT1  
Affinity Capture-MS Homo sapiens
13 Crtc2  
Co-fractionation Mus musculus
14 UGGT1 56886
Affinity Capture-MS Homo sapiens
15 TUBG1 7283
Affinity Capture-MS Homo sapiens
16 IMPDH1 3614
Affinity Capture-MS Homo sapiens
17 SIRT7  
Affinity Capture-MS Homo sapiens
18 Adar 56417
Co-fractionation Mus musculus
19 KIN  
Affinity Capture-MS Homo sapiens
20 DOCK5 80005
Affinity Capture-MS Homo sapiens
21 Siglece  
Reconstituted Complex Mus musculus
22 RPL37A 6168
Affinity Capture-MS Homo sapiens
23 SAP18 10284
Affinity Capture-MS Homo sapiens
24 ALDH1B1 219
Affinity Capture-MS Homo sapiens
25 TUBGCP3 10426
Affinity Capture-MS Homo sapiens
26 SKP1 6500
Affinity Capture-MS Homo sapiens
27 Helz2  
Co-fractionation Mus musculus
28 SLC26A2 1836
Affinity Capture-MS Homo sapiens
29 RICTOR 253260
Affinity Capture-MS Homo sapiens
30 FBXO45 200933
Affinity Capture-MS Homo sapiens
31 C4BPA 722
Affinity Capture-MS Homo sapiens
32 Rsad2  
Co-fractionation Mus musculus
33 GADD45GIP1  
Affinity Capture-MS Homo sapiens
34 SDCCAG3  
Affinity Capture-MS Homo sapiens
35 DERA 51071
Affinity Capture-MS Homo sapiens
36 ALDH16A1 126133
Affinity Capture-MS Homo sapiens
37 Ifit3  
Co-fractionation Mus musculus
38 CPS1 1373
Affinity Capture-MS Homo sapiens
39 ACIN1 22985
Affinity Capture-MS Homo sapiens
40 PPP1R9A  
Affinity Capture-MS Homo sapiens
41 Lgals9 16859
Co-fractionation Mus musculus
42 GANAB 23193
Affinity Capture-MS Homo sapiens
43 SDF2L1 23753
Affinity Capture-MS Homo sapiens
44 PC 5091
Affinity Capture-MS Homo sapiens
45 Cd248 70445
Co-fractionation Mus musculus
46 CAD 790
Affinity Capture-MS Homo sapiens
47 COL4A5  
Affinity Capture-MS Homo sapiens
48 HELZ 9931
Affinity Capture-MS Homo sapiens
49 SPRYD3 84926
Affinity Capture-MS Homo sapiens
50 ZNF281  
Affinity Capture-MS Homo sapiens
51 PRSS56  
Affinity Capture-MS Homo sapiens
52 PARP4 143
Affinity Capture-MS Homo sapiens
53 COLGALT1 79709
Affinity Capture-MS Homo sapiens
54 MZT2A  
Affinity Capture-MS Homo sapiens
55 Oas2  
Co-fractionation Mus musculus
56 MYL12A 10627
Affinity Capture-MS Homo sapiens
57 MZT2B 80097
Affinity Capture-MS Homo sapiens
58 RAE1 8480
Affinity Capture-MS Homo sapiens
59 Kif3c  
Co-fractionation Mus musculus
60 ABHD14A  
Affinity Capture-MS Homo sapiens
61 TUBGCP6 85378
Affinity Capture-MS Homo sapiens
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