Gene description for Lgals9
Gene name lectin, galactose binding, soluble 9
Gene symbol Lgals9
Other names/aliases AA407335
AI194909
AI265545
LGALS35
Lgals5
gal-9
galectin-9
Species Mus musculus
 Database cross references - Lgals9
ExoCarta ExoCarta_16859
Vesiclepedia VP_16859
Entrez Gene 16859
UniProt O08573  
 Lgals9 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Lgals9
Molecular Function
    signaling receptor binding GO:0005102 IPI
    protein binding GO:0005515 IPI
    galactoside binding GO:0016936 IBA
    galactoside binding GO:0016936 IMP
    galactoside binding GO:0016936 ISA
    enzyme binding GO:0019899 IPI
    enzyme binding GO:0019899 ISO
    carbohydrate binding GO:0030246 IBA
    carbohydrate binding GO:0030246 IDA
    carbohydrate binding GO:0030246 IMP
    carbohydrate binding GO:0030246 ISO
    carbohydrate binding GO:0030246 ISS
    protein serine/threonine kinase activator activity GO:0043539 IDA
    disaccharide binding GO:0048030 ISO
Biological Process
    positive regulation of cytokine production GO:0001819 IMP
    immune system process GO:0002376 IEA
    chemotaxis GO:0006935 IEA
    heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules GO:0007157 ISA
    transforming growth factor beta receptor signaling pathway GO:0007179 IMP
    female pregnancy GO:0007565 IDA
    positive regulation of gene expression GO:0010628 IBA
    negative regulation of gene expression GO:0010629 IMP
    regulation of T cell chemotaxis GO:0010819 IDA
    response to lipopolysaccharide GO:0032496 IMP
    negative regulation of type II interferon production GO:0032689 IBA
    negative regulation of type II interferon production GO:0032689 IDA
    negative regulation of type II interferon production GO:0032689 IMP
    positive regulation of chemokine production GO:0032722 IMP
    positive regulation of interleukin-1 production GO:0032732 IMP
    positive regulation of interleukin-10 production GO:0032733 IMP
    positive regulation of interleukin-6 production GO:0032755 IMP
    positive regulation of tumor necrosis factor production GO:0032760 IMP
    negative regulation of natural killer cell activation GO:0032815 IMP
    regulation of natural killer cell differentiation GO:0032823 IMP
    regulation of T cell differentiation in thymus GO:0033081 IMP
    positive regulation of macrophage activation GO:0043032 IMP
    receptor clustering GO:0043113 IDA
    negative regulation of natural killer cell degranulation GO:0043322 IMP
    positive regulation of innate immune response GO:0045089 IMP
    maintenance of protein location GO:0045185 IDA
    positive regulation of regulatory T cell differentiation GO:0045591 IMP
    negative regulation of inflammatory response GO:0050728 IMP
    positive regulation of oxidoreductase activity GO:0051353 IDA
    positive regulation of SMAD protein signal transduction GO:0060391 IMP
    cellular response to virus GO:0098586 IMP
    positive regulation of defense response to bacterium GO:1900426 IMP
    regulation of T-helper 17 type immune response GO:2000316 IMP
    positive regulation of T cell migration GO:2000406 IDA
    negative regulation of CD4-positive, alpha-beta T cell proliferation GO:2000562 IBA
    negative regulation of CD4-positive, alpha-beta T cell proliferation GO:2000562 IMP
    positive regulation of cysteine-type endopeptidase activity GO:2001056 ISO
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 ISO
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    collagen-containing extracellular matrix GO:0062023 HDA
 Experiment description of studies that identified Lgals9 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Lgals9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ifit3  
Co-fractionation Mus musculus
2 Atg16l1  
Affinity Capture-MS Mus musculus
3 Rsad2  
Co-fractionation Mus musculus
4 Lgals3bp 19039
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Lgals9 is involved
No pathways found





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