Gene description for Hspa5
Gene name heat shock protein 5
Gene symbol Hspa5
Other names/aliases AL022860
AU019543
Bip
D2Wsu141e
D2Wsu17e
Grp78
Hsce70
SEZ-7
Sez7
baffled
mBiP
Species Mus musculus
 Database cross references - Hspa5
ExoCarta ExoCarta_14828
Vesiclepedia VP_14828
Entrez Gene 14828
UniProt P20029  
 Hspa5 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Mast cells 17486113    
Oligodendrocytes 21136642    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Hspa5
Molecular Function
    DNA clamp loader activity GO:0003689 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    ATP hydrolysis activity GO:0016887 IBA
    ATP hydrolysis activity GO:0016887 ISO
    ATP hydrolysis activity GO:0016887 ISS
    enzyme binding GO:0019899 ISO
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 ISO
    protein serine/threonine kinase inhibitor activity GO:0030291 IEA
    heat shock protein binding GO:0031072 IBA
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    ribosome binding GO:0043022 IDA
    protein folding chaperone GO:0044183 IBA
    protein folding chaperone GO:0044183 IDA
    unfolded protein binding GO:0051082 ISO
    misfolded protein binding GO:0051787 IDA
    misfolded protein binding GO:0051787 ISO
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent protein folding chaperone GO:0140662 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    protein folding GO:0006457 IEA
    ER overload response GO:0006983 IDA
    positive regulation of neuron projection development GO:0010976 ISO
    cerebellum structural organization GO:0021589 IMP
    cerebellar Purkinje cell layer development GO:0021680 IMP
    positive regulation of cell migration GO:0030335 ISO
    positive regulation of cell migration GO:0030335 ISS
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 IGI
    endoplasmic reticulum unfolded protein response GO:0030968 IBA
    endoplasmic reticulum unfolded protein response GO:0030968 IMP
    post-translational protein targeting to membrane, translocation GO:0031204 ISO
    post-translational protein targeting to membrane, translocation GO:0031204 ISS
    negative regulation of protein-containing complex assembly GO:0031333 ISS
    positive regulation of protein ubiquitination GO:0031398 IMP
    response to endoplasmic reticulum stress GO:0034976 ISO
    maintenance of protein localization in endoplasmic reticulum GO:0035437 ISO
    maintenance of protein localization in endoplasmic reticulum GO:0035437 ISS
    IRE1-mediated unfolded protein response GO:0036498 IEP
    ERAD pathway GO:0036503 IBA
    positive regulation of embryonic development GO:0040019 TAS
    protein refolding GO:0042026 IBA
    cellular response to glucose starvation GO:0042149 IEA
    cellular response to glucose starvation GO:0042149 ISO
    negative regulation of apoptotic process GO:0043066 IEA
    negative regulation of apoptotic process GO:0043066 ISO
    chaperone cofactor-dependent protein refolding GO:0051085 IBA
    proteolysis involved in protein catabolic process GO:0051603 IDA
    cellular response to interleukin-4 GO:0071353 IDA
    chromatin looping GO:0140588 IEA
    negative regulation of IRE1-mediated unfolded protein response GO:1903895 ISS
    negative regulation of PERK-mediated unfolded protein response GO:1903898 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 ISO
    mitochondrion GO:0005739 ISS
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum lumen GO:0005788 IBA
    endoplasmic reticulum lumen GO:0005788 IDA
    endoplasmic reticulum lumen GO:0005788 ISO
    endoplasmic reticulum lumen GO:0005788 TAS
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 ISO
    smooth endoplasmic reticulum GO:0005790 ISO
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 IDA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 ISO
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    plasma membrane GO:0005886 IDA
    COP9 signalosome GO:0008180 IEA
    COP9 signalosome GO:0008180 ISO
    cell surface GO:0009986 IDA
    membrane GO:0016020 IBA
    membrane GO:0016020 ISO
    midbody GO:0030496 IEA
    midbody GO:0030496 ISO
    protein-containing complex GO:0032991 ISO
    endoplasmic reticulum chaperone complex GO:0034663 IBA
    endoplasmic reticulum chaperone complex GO:0034663 IDA
    endoplasmic reticulum chaperone complex GO:0034663 ISO
    melanosome GO:0042470 IEA
    neuronal cell body GO:0043025 ISO
    dendritic shaft GO:0043198 ISO
    myelin sheath GO:0043209 HDA
    intracellular membrane-bounded organelle GO:0043231 ISO
 Experiment description of studies that identified Hspa5 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
5
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hspa5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Erp29 67397
Co-purification Mus musculus
2 Kctd13  
Affinity Capture-MS Mus musculus
3 Elf5  
Affinity Capture-MS Mus musculus
4 SNCA 6622
Affinity Capture-MS Homo sapiens
5 Ybx3  
Co-fractionation Mus musculus
6 Tubgcp2  
Co-fractionation Mus musculus
7 Prnp 19122
Affinity Capture-MS Mus musculus
8 Dnajc10  
Affinity Capture-Western Mus musculus
9 SOD1 6647
Affinity Capture-MS Homo sapiens
10 Pdia2 69191
Co-purification Mus musculus
11 Kcnma1  
Affinity Capture-MS Mus musculus
12 Tmem173  
Proximity Label-MS Mus musculus
13 Ahi1  
Affinity Capture-MS Mus musculus
14 Poglut1  
Co-purification Mus musculus
15 Cebpa  
Affinity Capture-MS Mus musculus
16 Dnajb11  
Co-purification Mus musculus
17 Hsp90b1 22027
Co-purification Mus musculus
18 Tfe3  
Affinity Capture-MS Mus musculus
19 SNCAIP  
Affinity Capture-Western Homo sapiens
20 Mks1  
Affinity Capture-MS Mus musculus
21 Iqcb1  
Affinity Capture-MS Mus musculus
22 Ywhaz 22631
Affinity Capture-MS Mus musculus
23 Myd88  
Affinity Capture-MS Mus musculus
24 Nphp4  
Affinity Capture-MS Mus musculus
25 Fzr1  
Affinity Capture-MS Mus musculus
26 Pdia4 12304
Co-purification Mus musculus
27 Sigmar1  
Affinity Capture-Western Mus musculus
28 Sdf2l1  
Co-purification Mus musculus
Co-fractionation Mus musculus
29 Traf6  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
30 Os9  
Co-fractionation Mus musculus
31 Dnajc1  
Affinity Capture-MS Mus musculus
Reconstituted Complex Mus musculus
32 Snw1  
Co-fractionation Mus musculus
33 Uchl5 56207
Co-fractionation Mus musculus
34 Invs  
Affinity Capture-MS Mus musculus
35 Sfpq 71514
Co-fractionation Mus musculus
36 STMN1 3925
Two-hybrid Homo sapiens
37 Ighm 16019
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
38 Sec23b  
Co-fractionation Mus musculus
39 Eed  
Affinity Capture-MS Mus musculus
40 Hspa5 14828
Co-purification Mus musculus
Co-purification Mus musculus
41 Nlrp3  
Affinity Capture-MS Mus musculus
42 Hyou1 12282
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
43 Set 56086
Affinity Capture-MS Mus musculus
44 Nphp1  
Affinity Capture-MS Mus musculus
45 App 11820
Affinity Capture-MS Mus musculus
46 Fancd2  
Affinity Capture-MS Mus musculus
47 ZC3HAV1 56829
Two-hybrid Homo sapiens
48 Lipc  
Affinity Capture-MS Mus musculus
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