Gene description for Hspa5
Gene name heat shock protein 5
Gene symbol Hspa5
Other names/aliases AL022860
AU019543
Bip
D2Wsu141e
D2Wsu17e
Grp78
Hsce70
SEZ-7
Sez7
baffled
mBiP
Species Mus musculus
 Database cross references - Hspa5
ExoCarta ExoCarta_14828
Entrez Gene 14828
UniProt P20029  
 Hspa5 identified in exosomes derived from the following tissue/cell type
Fibroblasts 23260141    
Mast cells 17486113    
Oligodendrocytes 21136642    
Pancreatic cells 19351151    
 Gene ontology annotations for Hspa5
Molecular Function
    enzyme binding GO:0019899 ISO
    misfolded protein binding GO:0051787 ISO
    ubiquitin protein ligase binding GO:0031625 ISO
    ATP binding GO:0005524 IEA
    glycoprotein binding GO:0001948 ISO
    unfolded protein binding GO:0051082 ISO
    ribosome binding GO:0043022 IDA
    protein binding GO:0005515 IPI
    nucleotide binding GO:0000166 IEA
    ATPase activity GO:0016887 ISO
    protein domain specific binding GO:0019904 ISO
Biological Process
    maintenance of protein localization in endoplasmic reticulum GO:0035437 ISO
    proteolysis involved in cellular protein catabolic process GO:0051603 IDA
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 IGI
    cerebellar Purkinje cell layer development GO:0021680 IMP
    toxin transport GO:1901998 IMP
    positive regulation of protein ubiquitination GO:0031398 IMP
    positive regulation of embryonic development GO:0040019 TAS
    positive regulation of cell migration GO:0030335 ISO
    cellular response to glucose starvation GO:0042149 ISO
    cerebellum structural organization GO:0021589 IMP
    response to endoplasmic reticulum stress GO:0034976 ISO
    activation of signaling protein activity involved in unfolded protein response GO:0006987 IMP
    cellular response to interleukin-4 GO:0071353 IDA
    negative regulation of apoptotic process GO:0043066 ISO
    ER overload response GO:0006983 IDA
Subcellular Localization
    focal adhesion GO:0005925 ISO
    cell surface GO:0009986 IDA
    plasma membrane GO:0005886 IDA
    nucleus GO:0005634 ISO
    smooth endoplasmic reticulum GO:0005790 ISO
    cytoplasm GO:0005737 IEA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 ISO
    endoplasmic reticulum chaperone complex GO:0034663 ISO
    midbody GO:0030496 ISO
    mitochondrion GO:0005739 ISO
    endoplasmic reticulum lumen GO:0005788 IDA
    endoplasmic reticulum GO:0005783 ISO
    COP9 signalosome GO:0008180 ISO
    extracellular exosome GO:0070062 ISO
    membrane GO:0016020 ISO
    myelin sheath GO:0043209 IDA
    endoplasmic reticulum membrane GO:0005789 IDA
    integral component of endoplasmic reticulum membrane GO:0030176 ISO
 Experiment description of studies that identified Hspa5 in exosomes
1
Experiment ID 210
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD81|FLOT1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 33
ISEV standards
EM|IEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
4
Experiment ID 188
ISEV standards
EM
EV Biophysical techniques
GAPDH|UCHL1|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Hspa5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Dnajc1  
Reconstituted Complex Mus musculus
2 STMN1 3925
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which Hspa5 is involved
PathwayEvidenceSource
Platelet degranulation IEA Reactome





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