Gene description for Plec
Gene name plectin
Gene symbol Plec
Other names/aliases AA591047
AU042537
EBS1
PCN
PLTN
Plec1
Species Mus musculus
 Database cross references - Plec
ExoCarta ExoCarta_18810
Vesiclepedia VP_18810
Entrez Gene 18810
UniProt Q9QXS1  
 Plec identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Plec
Molecular Function
    dystroglycan binding GO:0002162 IDA
    actin binding GO:0003779 IDA
    structural constituent of cytoskeleton GO:0005200 IBA
    structural constituent of cytoskeleton GO:0005200 ISO
    protein binding GO:0005515 IPI
    cytoskeletal protein binding GO:0008092 ISO
    structural constituent of muscle GO:0008307 ISO
    ankyrin binding GO:0030506 IBA
    ankyrin binding GO:0030506 IEA
    ankyrin binding GO:0030506 ISO
    identical protein binding GO:0042802 IDA
    actin filament binding GO:0051015 IDA
Biological Process
    cell morphogenesis GO:0000902 IMP
    leukocyte migration involved in immune response GO:0002522 IMP
    keratinocyte development GO:0003334 IMP
    negative regulation of protein kinase activity GO:0006469 IMP
    nucleus organization GO:0006997 IMP
    mitochondrion organization GO:0007005 IMP
    actin filament organization GO:0007015 IMP
    skeletal muscle tissue development GO:0007519 IMP
    response to nutrient GO:0007584 ISO
    protein localization GO:0008104 IMP
    gene expression GO:0010467 IMP
    fibroblast migration GO:0010761 IMP
    T cell chemotaxis GO:0010818 IMP
    skeletal myofibril assembly GO:0014866 IMP
    transmission of nerve impulse GO:0019226 IMP
    myelination in peripheral nervous system GO:0022011 IMP
    respiratory electron transport chain GO:0022904 IMP
    actin cytoskeleton organization GO:0030036 IMP
    keratinocyte differentiation GO:0030216 IMP
    hemidesmosome assembly GO:0031581 IBA
    hemidesmosome assembly GO:0031581 ISO
    hemidesmosome assembly GO:0031581 ISS
    response to food GO:0032094 IMP
    peripheral nervous system myelin maintenance GO:0032287 IMP
    adherens junction organization GO:0034332 IMP
    multicellular organism growth GO:0035264 IMP
    wound healing GO:0042060 IBA
    regulation of vascular permeability GO:0043114 IMP
    skin development GO:0043588 IMP
    protein-containing complex organization GO:0043933 IMP
    intermediate filament cytoskeleton organization GO:0045104 IBA
    intermediate filament cytoskeleton organization GO:0045104 IMP
    intermediate filament organization GO:0045109 IMP
    sarcomere organization GO:0045214 IMP
    myoblast differentiation GO:0045445 IMP
    skeletal muscle fiber development GO:0048741 IMP
    cell motility GO:0048870 IMP
    cellular response to stimulus GO:0051716 IMP
    cardiac muscle cell development GO:0055013 IMP
    establishment of skin barrier GO:0061436 IMP
    cellular response to mechanical stimulus GO:0071260 IMP
    cellular response to hydrostatic pressure GO:0071464 IMP
    cellular response to fluid shear stress GO:0071498 IMP
    tight junction organization GO:0120193 IMP
    actomyosin contractile ring assembly actin filament organization GO:2000689 IMP
    regulation of ATP citrate synthase activity GO:2000983 IMP
Subcellular Localization
    podosome GO:0002102 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    mitochondrial outer membrane GO:0005741 IMP
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    intermediate filament GO:0005882 IBA
    brush border GO:0005903 IDA
    focal adhesion GO:0005925 IBA
    focal adhesion GO:0005925 IEA
    focal adhesion GO:0005925 ISO
    basal plasma membrane GO:0009925 ISO
    apical plasma membrane GO:0016324 ISO
    sarcoplasm GO:0016528 ISO
    myofibril GO:0030016 IDA
    Z disc GO:0030018 IDA
    hemidesmosome GO:0030056 IBA
    hemidesmosome GO:0030056 IDA
    hemidesmosome GO:0030056 ISO
    hemidesmosome GO:0030056 ISS
    axon GO:0030424 IDA
    dendrite GO:0030425 IDA
    sarcolemma GO:0042383 IBA
    sarcolemma GO:0042383 IDA
    sarcolemma GO:0042383 ISO
    sarcolemma GO:0042383 ISS
    myelin sheath GO:0043209 IDA
    contractile muscle fiber GO:0043292 IDA
    intermediate filament cytoskeleton GO:0045111 IEA
    intermediate filament cytoskeleton GO:0045111 ISO
    perinuclear region of cytoplasm GO:0048471 IBA
    perinuclear region of cytoplasm GO:0048471 ISO
    cell periphery GO:0071944 IDA
 Experiment description of studies that identified Plec in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Plec
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Invs  
Affinity Capture-MS Mus musculus
2 Crk 12928
Affinity Capture-MS Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Mettl21e  
Affinity Capture-MS Mus musculus
5 Ubc  
Reconstituted Complex Mus musculus
6 Nphp4  
Affinity Capture-MS Mus musculus
7 Tmem173  
Proximity Label-MS Mus musculus
8 Foxp3  
Affinity Capture-MS Mus musculus
9 Eed  
Affinity Capture-MS Mus musculus
10 Thrb  
Affinity Capture-MS Mus musculus
11 Actg2 11468
Reconstituted Complex Mus musculus
12 Atg16l1  
Affinity Capture-MS Mus musculus
13 Nphp1  
Affinity Capture-MS Mus musculus
14 Iqcb1  
Affinity Capture-MS Mus musculus
15 Plec 18810
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
16 Fancd2  
Affinity Capture-MS Mus musculus
17 Ywhaz 22631
Affinity Capture-MS Mus musculus
18 HACE1 57531
Affinity Capture-MS Homo sapiens
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