Experiment description of studies that identified GNPTG in sEVs

1 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 66 MISEV standards ✔ IEM Biophysical techniques ✔ TSG101|Alix|CD63|CD81 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19199708     Organism Homo sapiens Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT). Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR" Journal name JPR Publication year 2009 Sample Saliva Sample name Saliva Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting Immunoelectron Microscopy

Protein-protein interactions for GNPTG

Protein Interactor ExoCarta ID Identification method PubMed Species 1 B3GALT2   Affinity Capture-MS Homo sapiens 2 GPIHBP1   Affinity Capture-MS Homo sapiens 3 Anapc2   Affinity Capture-MS Mus musculus 4 PICK1   Affinity Capture-MS Homo sapiens 5 ELSPBP1   Affinity Capture-MS Homo sapiens 6 GNPTAB 79158 Affinity Capture-MS Homo sapiens 7 PDYN   Affinity Capture-MS Homo sapiens 8 Cdc23   Affinity Capture-MS Mus musculus 9 TAZ   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 10 TMEM25   Affinity Capture-MS Homo sapiens 11 GGH 8836 Affinity Capture-MS Homo sapiens 12 ANAPC15   Affinity Capture-MS Homo sapiens 13 PRG2   Affinity Capture-MS Homo sapiens 14 LGALS1 3956 Affinity Capture-MS Homo sapiens 15 CBLN4   Affinity Capture-MS Homo sapiens 16 PLOD1 5351 Affinity Capture-MS Homo sapiens 17 OLFM4 10562 Affinity Capture-MS Homo sapiens 18 C5AR1   Affinity Capture-MS Homo sapiens 19 C1orf101   Affinity Capture-MS Homo sapiens 20 Cdc16   Affinity Capture-MS Mus musculus 21 SPSB3   Affinity Capture-MS Homo sapiens 22 FAM134C 162427 Affinity Capture-MS Homo sapiens 23 C22orf42   Affinity Capture-MS Homo sapiens 24 WDYHV1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 25 UPF1 5976 Two-hybrid Homo sapiens 26 PXYLP1   Affinity Capture-MS Homo sapiens 27 PTPRK 5796 Affinity Capture-MS Homo sapiens 28 SGCZ   Affinity Capture-MS Homo sapiens 29 ISLR 3671 Affinity Capture-MS Homo sapiens 30 ZPLD1   Affinity Capture-MS Homo sapiens 31 Cdc26   Affinity Capture-MS Mus musculus View the network

Pathways in which GNPTG is involved

No pathways found