Gene description for CDK4
Gene name cyclin-dependent kinase 4
Gene symbol CDK4
Other names/aliases CMM3
PSK-J3
Species Homo sapiens
 Database cross references - CDK4
ExoCarta ExoCarta_1019
Vesiclepedia VP_1019
Entrez Gene 1019
HGNC 1773
MIM 123829
UniProt P11802  
 CDK4 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for CDK4
Molecular Function
    cyclin-dependent protein serine/threonine kinase activity GO:0004693 IBA
    cyclin-dependent protein serine/threonine kinase activity GO:0004693 IDA
    cyclin-dependent protein serine/threonine kinase activity GO:0004693 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    cyclin-dependent protein serine/threonine kinase regulator activity GO:0016538 TAS
    cyclin binding GO:0030332 IBA
    cyclin binding GO:0030332 IPI
    protein serine kinase activity GO:0106310 IEA
Biological Process
    G1/S transition of mitotic cell cycle GO:0000082 IBA
    G1/S transition of mitotic cell cycle GO:0000082 IDA
    G1/S transition of mitotic cell cycle GO:0000082 IMP
    G1/S transition of mitotic cell cycle GO:0000082 NAS
    protein phosphorylation GO:0006468 IDA
    signal transduction GO:0007165 IBA
    positive regulation of cell population proliferation GO:0008284 IMP
    response to xenobiotic stimulus GO:0009410 IEP
    regulation of G2/M transition of mitotic cell cycle GO:0010389 IBA
    regulation of gene expression GO:0010468 IBA
    regulation of gene expression GO:0010468 IMP
    positive regulation of G2/M transition of mitotic cell cycle GO:0010971 IDA
    positive regulation of fibroblast proliferation GO:0048146 IMP
    cell division GO:0051301 IEA
    regulation of cell cycle GO:0051726 IDA
    regulation of transcription initiation by RNA polymerase II GO:0060260 TAS
    regulation of type B pancreatic cell proliferation GO:0061469 IEA
    cellular response to lipopolysaccharide GO:0071222 IEA
    cellular response to interleukin-4 GO:0071353 IEA
    cellular response to phorbol 13-acetate 12-myristate GO:1904628 IEA
    cellular response to ionomycin GO:1904637 IEA
Subcellular Localization
    cyclin-dependent protein kinase holoenzyme complex GO:0000307 IBA
    cyclin-dependent protein kinase holoenzyme complex GO:0000307 IDA
    chromatin GO:0000785 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    transcription regulator complex GO:0005667 IEA
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    bicellular tight junction GO:0005923 IEA
    nuclear membrane GO:0031965 IDA
    cyclin D1-CDK4 complex GO:0097128 IPI
    cyclin D2-CDK4 complex GO:0097129 IPI
    cyclin D3-CDK4 complex GO:0097130 IPI
 Experiment description of studies that identified CDK4 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CDK4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CNOT7 29883
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
2 PSMA6 5687
Affinity Capture-Western Homo sapiens
3 RPRM  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 UBE2H 7328
Affinity Capture-MS Homo sapiens
5 SMC3 9126
Affinity Capture-MS Homo sapiens
6 SENP3 26168
Biochemical Activity Homo sapiens
7 HOOK1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
8 QARS 5859
Two-hybrid Homo sapiens
9 GEMIN4 50628
Affinity Capture-MS Homo sapiens
10 TK1 7083
Two-hybrid Homo sapiens
11 CDK2 1017
Co-fractionation Homo sapiens
12 MARCKS 4082
Biochemical Activity Homo sapiens
13 PTMA 5757
Co-purification Homo sapiens
14 RIN3  
Affinity Capture-MS Homo sapiens
15 UBXN10  
Affinity Capture-MS Homo sapiens
16 RIT1 6016
Negative Genetic Homo sapiens
17 TARDBP 23435
Affinity Capture-MS Homo sapiens
18 HIST1H1D 3007
Biochemical Activity Homo sapiens
19 SP1  
Affinity Capture-Western Homo sapiens
20 ZC3H14  
Affinity Capture-MS Homo sapiens
21 APC  
Negative Genetic Homo sapiens
22 BRCA1 672
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
23 APLP1 333
Two-hybrid Homo sapiens
24 MTMR14 64419
Affinity Capture-MS Homo sapiens
25 SLC25A1 6576
Affinity Capture-MS Homo sapiens
26 CHEK2  
Negative Genetic Homo sapiens
27 VHL  
Negative Genetic Homo sapiens
28 PSMD10 5716
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
29 PTPN1 5770
Affinity Capture-MS Homo sapiens
30 SETDB1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
31 PRKDC 5591
Negative Genetic Homo sapiens
32 APP 351
Reconstituted Complex Homo sapiens
33 MYOD1  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
34 CUL4B 8450
Affinity Capture-MS Homo sapiens
35 CAPNS1 826
Two-hybrid Homo sapiens
36 IGF2BP3 10643
Affinity Capture-RNA Homo sapiens
37 PPP2R1B 5519
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
38 MRPS34 65993
Affinity Capture-MS Homo sapiens
39 USP17L2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
40 MCM2 4171
Reconstituted Complex Homo sapiens
41 COPS6 10980
Affinity Capture-MS Homo sapiens
42 COPS5 10987
Affinity Capture-MS Homo sapiens
43 SMTN 6525
Affinity Capture-MS Homo sapiens
44 IQGAP1 8826
Affinity Capture-MS Homo sapiens
45 PSMD9 5715
Affinity Capture-Western Homo sapiens
46 SSR1 6745
Affinity Capture-MS Homo sapiens
47 SLBP  
Biochemical Activity Homo sapiens
48 PGD 5226
Two-hybrid Homo sapiens
49 CDKN1C  
Affinity Capture-MS Homo sapiens
50 DSG1 1828
Affinity Capture-MS Homo sapiens
51 CDT1  
Affinity Capture-Western Homo sapiens
52 ZNF219  
Biochemical Activity Homo sapiens
53 OGDHL  
Two-hybrid Homo sapiens
54 RPL34 6164
Reconstituted Complex Homo sapiens
55 CUL1 8454
Affinity Capture-MS Homo sapiens
56 NPAS1  
Affinity Capture-MS Homo sapiens
57 ERBB2 2064
Negative Genetic Homo sapiens
58 CDK13 8621
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 TRAP1 10131
Affinity Capture-MS Homo sapiens
60 HSPA8 3312
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
61 ANKRD12  
Biochemical Activity Homo sapiens
62 VTN 7448
Affinity Capture-MS Homo sapiens
63 RB1 5925
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
64 E2F4  
Affinity Capture-MS Homo sapiens
65 PPP1CA 5499
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
66 ANXA7 310
Two-hybrid Homo sapiens
67 POLD1 5424
Biochemical Activity Homo sapiens
68 MTMR8  
Affinity Capture-MS Homo sapiens
69 DBNL 28988
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 PKM 5315
Two-hybrid Homo sapiens
71 VSIG8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 TMEM126A 84233
Affinity Capture-MS Homo sapiens
73 FKBP6  
Affinity Capture-MS Homo sapiens
74 CDKN1A  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
75 ZBTB16  
Two-hybrid Homo sapiens
76 LYN 4067
Affinity Capture-MS Homo sapiens
77 BAG6 7917
Two-hybrid Homo sapiens
78 DAZAP2  
Two-hybrid Homo sapiens
79 PTPN21 11099
Affinity Capture-MS Homo sapiens
80 ACTR3B 57180
Affinity Capture-MS Homo sapiens
81 SERTAD1  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
82 VCP 7415
Affinity Capture-MS Homo sapiens
83 NTRK1 4914
Affinity Capture-MS Homo sapiens
84 SMAD3 4088
Biochemical Activity Homo sapiens
Co-localization Homo sapiens
85 CDH1 999
Affinity Capture-Western Homo sapiens
86 LUC7L2 51631
Two-hybrid Homo sapiens
87 SMAD2 4087
Biochemical Activity Homo sapiens
88 CIB1 10519
Two-hybrid Homo sapiens
89 UBTF 7343
Biochemical Activity Homo sapiens
90 SMC1A 8243
Affinity Capture-MS Homo sapiens
91 RCAN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 CEBPA  
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
93 ATP5B 506
Two-hybrid Homo sapiens
94 GUSB 2990
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 ARID4A  
Biochemical Activity Homo sapiens
96 SHOX2  
Biochemical Activity Homo sapiens
97 DDAH2 23564
Two-hybrid Homo sapiens
98 RBL1  
Biochemical Activity Homo sapiens
99 FBXO8  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
100 FBXO4 26272
Affinity Capture-Western Homo sapiens
101 BCL11A 53335
Biochemical Activity Homo sapiens
102 FNIP1  
Affinity Capture-Western Homo sapiens
103 ZNF101  
Biochemical Activity Homo sapiens
104 IL15RA 3601
Two-hybrid Homo sapiens
105 CCNA2 890
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
106 GFER  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 AK2 204
Affinity Capture-MS Homo sapiens
108 PAQR4  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
109 SKP2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
110 IKZF3  
Two-hybrid Homo sapiens
111 TSPYL2  
Biochemical Activity Homo sapiens
112 ASPM 259266
Affinity Capture-MS Homo sapiens
113 WDR33 55339
Biochemical Activity Homo sapiens
114 OGFRL1 79627
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
115 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
116 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
117 PTEN 5728
Positive Genetic Homo sapiens
118 CCNE1  
Affinity Capture-Western Homo sapiens
119 UHRF2  
Far Western Homo sapiens
120 GLI1  
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
121 HSP90AB3P 3327
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
122 FARP2 9855
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
123 PTK2 5747
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
124 HSP90AA5P 730211
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
125 MYC  
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Negative Genetic Homo sapiens
126 RFC1 5981
Affinity Capture-Western Homo sapiens
127 CDK5R1  
Affinity Capture-Western Homo sapiens
128 PRMT5 10419
Affinity Capture-Western Homo sapiens
129 FKBP5 2289
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
130 H1F0 3005
Biochemical Activity Homo sapiens
131 ANKRD52 283373
Affinity Capture-MS Homo sapiens
132 PIAS1 8554
Two-hybrid Homo sapiens
133 CDKN2B 1030
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
134 RPA3 6119
Proximity Label-MS Homo sapiens
135 IRF8  
Affinity Capture-MS Homo sapiens
136 EIF4EBP2 1979
Two-hybrid Homo sapiens
137 DNAJC9 23234
Affinity Capture-MS Homo sapiens
138 RBL2 5934
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
139 TOP1 7150
Negative Genetic Homo sapiens
140 HMGXB3  
Two-hybrid Homo sapiens
141 HIST1H1A 3024
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
142 DUSP9  
Two-hybrid Homo sapiens
143 SAP25  
Affinity Capture-MS Homo sapiens
144 CDKN2D  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
145 CDKN1B 1027
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
146 CCND1 595
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
147 CDC7  
Reconstituted Complex Homo sapiens
148 MZF1  
Biochemical Activity Homo sapiens
149 UBXN1 51035
Affinity Capture-MS Homo sapiens
150 CDKN2C  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
151 HUWE1 10075
Affinity Capture-MS Homo sapiens
152 BIRC5  
Affinity Capture-Western Homo sapiens
153 STUB1 10273
Biochemical Activity Homo sapiens
154 PRR14L 253143
Affinity Capture-MS Homo sapiens
155 RELA 5970
Affinity Capture-Western Homo sapiens
156 SNCA 6622
Two-hybrid Homo sapiens
157 FGR 2268
Affinity Capture-MS Homo sapiens
158 ARRB2 409
Affinity Capture-MS Homo sapiens
159 FASN 2194
Positive Genetic Homo sapiens
160 SPOP  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
161 ZNFX1 57169
Affinity Capture-MS Homo sapiens
162 CCND2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
163 NUP133 55746
Affinity Capture-MS Homo sapiens
164 WDR77 79084
Affinity Capture-Western Homo sapiens
165 SORBS1 10580
Affinity Capture-MS Homo sapiens
166 TP53 7157
Negative Genetic Homo sapiens
167 EIF1AX 1964
Affinity Capture-MS Homo sapiens
168 TRMT2A 27037
Two-hybrid Homo sapiens
169 INCA1  
Two-hybrid Homo sapiens
170 CDC37 11140
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
PCA Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
171 SKP1 6500
Two-hybrid Homo sapiens
172 NCOA2 10499
Reconstituted Complex Homo sapiens
173 MAPRE2 10982
Two-hybrid Homo sapiens
174 CDC6  
Biochemical Activity Homo sapiens
175 ZNF335 63925
Biochemical Activity Homo sapiens
176 HDAC1 3065
Negative Genetic Homo sapiens
177 VTA1 51534
Two-hybrid Homo sapiens
178 FNIP2  
Affinity Capture-Western Homo sapiens
179 HSP90AA1 3320
Affinity Capture-Western Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
180 CDK6 1021
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
181 DDX58 23586
Affinity Capture-RNA Homo sapiens
182 CDK1 983
Co-fractionation Homo sapiens
183 MAPK1 5594
Affinity Capture-MS Homo sapiens
184 DCAF6 55827
Affinity Capture-MS Homo sapiens
185 PCNA 5111
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
186 UFL1 23376
Affinity Capture-MS Homo sapiens
187 JAK3 3718
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
188 ZNF655  
Two-hybrid Homo sapiens
189 OGFR 11054
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
190 BCL2  
Negative Genetic Homo sapiens
191 EGLN2  
Affinity Capture-Western Homo sapiens
192 C16orf72 29035
Affinity Capture-MS Homo sapiens
193 YBX3 8531
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
194 FOXM1  
Biochemical Activity Homo sapiens
195 SERBP1 26135
Affinity Capture-MS Homo sapiens
196 C9orf72  
Affinity Capture-MS Homo sapiens
197 HSP90AB4P 664618
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
198 PPP2R1A 5518
Affinity Capture-Western Homo sapiens
199 LIMK2 3985
Affinity Capture-MS Homo sapiens
200 CDKN2A 1029
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
201 OTX2  
Biochemical Activity Homo sapiens
202 CCND3  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which CDK4 is involved
PathwayEvidenceSource
Aberrant regulation of mitotic cell cycle due to RB1 defects TAS Reactome
Aberrant regulation of mitotic G1/S transition in cancer due to RB1 defects TAS Reactome
Adipogenesis TAS Reactome
Cell Cycle TAS Reactome
Cell Cycle IEA Reactome
Cell Cycle, Mitotic TAS Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Cellular Senescence TAS Reactome
Chromatin modifying enzymes TAS Reactome
Chromatin organization TAS Reactome
Cyclin A:Cdk2-associated events at S phase entry TAS Reactome
Cyclin D associated events in G1 TAS Reactome
Cyclin E associated events during G1/S transition TAS Reactome
Defective binding of RB1 mutants to E2F1,(E2F2, E2F3) TAS Reactome
Developmental Biology TAS Reactome
Disease TAS Reactome
Diseases of cellular response to stress TAS Reactome
Diseases of Cellular Senescence TAS Reactome
Diseases of mitotic cell cycle TAS Reactome
Drug-mediated inhibition of CDK4/CDK6 activity TAS Reactome
Evasion of Oncogene Induced Senescence Due to Defective p16INK4A binding to CDK4 TAS Reactome
Evasion of Oncogene Induced Senescence Due to Defective p16INK4A binding to CDK4 and CDK6 TAS Reactome
Evasion of Oncogene Induced Senescence Due to p16INK4A Defects TAS Reactome
Evasion of Oxidative Stress Induced Senescence Due to Defective p16INK4A binding to CDK4 TAS Reactome
Evasion of Oxidative Stress Induced Senescence Due to Defective p16INK4A binding to CDK4 and CDK6 TAS Reactome
Evasion of Oxidative Stress Induced Senescence Due to p16INK4A Defects TAS Reactome
G1 Phase TAS Reactome
G1/S Transition TAS Reactome
Gene expression (Transcription) TAS Reactome
Generic Transcription Pathway TAS Reactome
Meiosis IEA Reactome
Meiotic recombination IEA Reactome
Mitotic G1 phase and G1/S transition TAS Reactome
Oncogene Induced Senescence TAS Reactome
Oxidative Stress Induced Senescence TAS Reactome
PTK6 Regulates Cell Cycle TAS Reactome
Reproduction IEA Reactome
RMTs methylate histone arginines TAS Reactome
RNA Polymerase II Transcription TAS Reactome
S Phase IEA Reactome
S Phase TAS Reactome
SCF(Skp2)-mediated degradation of p27/p21 TAS Reactome
Senescence-Associated Secretory Phenotype (SASP) TAS Reactome
Signal Transduction TAS Reactome
Signaling by Non-Receptor Tyrosine Kinases TAS Reactome
Signaling by PTK6 TAS Reactome
Transcriptional regulation by RUNX2 TAS Reactome
Transcriptional regulation of granulopoiesis TAS Reactome
Transcriptional regulation of white adipocyte differentiation TAS Reactome
Ubiquitin-dependent degradation of Cyclin D IEA Reactome
Ubiquitin-dependent degradation of Cyclin D TAS Reactome





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