Gene description for WDR33
Gene name WD repeat domain 33
Gene symbol WDR33
Other names/aliases NET14
WDC146
Species Homo sapiens
 Database cross references - WDR33
ExoCarta ExoCarta_55339
Vesiclepedia VP_55339
Entrez Gene 55339
HGNC 25651
UniProt Q9C0J8  
 WDR33 identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for WDR33
Molecular Function
    RNA binding GO:0003723 HDA
Biological Process
    postreplication repair GO:0006301 NAS
    spermatogenesis GO:0007283 NAS
    mRNA 3'-end processing GO:0031124 IEA
Subcellular Localization
    fibrillar center GO:0001650 IDA
    collagen trimer GO:0005581 IEA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    mRNA cleavage and polyadenylation specificity factor complex GO:0005847 IBA
 Experiment description of studies that identified WDR33 in sEVs
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for WDR33
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 ISG15 9636
Affinity Capture-MS Homo sapiens
3 MED17  
Co-fractionation Homo sapiens
4 EEF1G 1937
Two-hybrid Homo sapiens
5 PCF11 51585
Co-fractionation Homo sapiens
6 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
7 RPA2 6118
Affinity Capture-MS Homo sapiens
8 P4HA2 8974
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 DHX8 1659
Proximity Label-MS Homo sapiens
10 KIF20A 10112
Affinity Capture-MS Homo sapiens
11 UBC 7316
Reconstituted Complex Homo sapiens
12 CPSF4L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 GSK3A 2931
Affinity Capture-MS Homo sapiens
14 KAT7  
Two-hybrid Homo sapiens
15 CPSF2 53981
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
16 SNRNP70 6625
Affinity Capture-MS Homo sapiens
17 RNPS1 10921
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 KIF23 9493
Affinity Capture-MS Homo sapiens
19 S100P 6286
Affinity Capture-MS Homo sapiens
20 CPSF4  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 Htatsf1  
Two-hybrid Mus musculus
22 C8orf82  
Proximity Label-MS Homo sapiens
23 NPM1 4869
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
24 UFL1 23376
Affinity Capture-MS Homo sapiens
25 Tagap  
Affinity Capture-MS Mus musculus
26 TIAM1  
Affinity Capture-MS Homo sapiens
27 MDC1  
Affinity Capture-MS Homo sapiens
28 ATG16L1 55054
Affinity Capture-MS Homo sapiens
29 PFN2 5217
Two-hybrid Homo sapiens
30 MECP2 4204
Affinity Capture-MS Homo sapiens
31 WASF3 10810
Affinity Capture-MS Homo sapiens
32 RBM14 10432
Affinity Capture-MS Homo sapiens
33 KIF14 9928
Affinity Capture-MS Homo sapiens
34 DYRK4  
Affinity Capture-MS Homo sapiens
35 CCT2 10576
Affinity Capture-MS Homo sapiens
36 VTN 7448
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 CLK2 1196
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 JMJD6 23210
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 SYMPK 8189
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 P4HA1 5033
Affinity Capture-MS Homo sapiens
41 UTP14A 10813
Two-hybrid Homo sapiens
42 Anapc5 59008
Affinity Capture-MS Mus musculus
43 ZBTB16  
Two-hybrid Homo sapiens
44 CSTF2 1478
Affinity Capture-MS Homo sapiens
45 TRA2A 29896
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 SRSF6 6431
Affinity Capture-MS Homo sapiens
47 SH3GL3  
Two-hybrid Homo sapiens
48 Vav2  
Affinity Capture-MS Mus musculus
49 GIT1 28964
Two-hybrid Homo sapiens
50 BARD1 580
Affinity Capture-MS Homo sapiens
51 SERPINB8 5271
Affinity Capture-MS Homo sapiens
52 DTX2 113878
Proximity Label-MS Homo sapiens
53 ARRDC3 57561
Affinity Capture-MS Homo sapiens
54 CAPN1 823
Affinity Capture-MS Homo sapiens
55 KDF1  
Affinity Capture-MS Homo sapiens
56 CPSF1 29894
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
57 SMARCAD1  
Affinity Capture-MS Homo sapiens
58 AHCYL1 10768
Affinity Capture-MS Homo sapiens
59 ZHX1  
Two-hybrid Homo sapiens
60 ECT2 1894
Affinity Capture-MS Homo sapiens
61 ZNF622 90441
Co-fractionation Homo sapiens
62 EGFR 1956
Negative Genetic Homo sapiens
63 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
64 FUS 2521
Affinity Capture-MS Homo sapiens
65 SYDE1 85360
Affinity Capture-MS Homo sapiens
66 LZTS2 84445
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 PCBP1 5093
Affinity Capture-MS Homo sapiens
68 MYO6 4646
Proximity Label-MS Homo sapiens
69 RPA3 6119
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
70 ARHGAP4 393
Affinity Capture-MS Homo sapiens
71 ZC3H18  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 SEC16A 9919
Affinity Capture-MS Homo sapiens
73 TOP1 7150
Affinity Capture-MS Homo sapiens
74 FIP1L1 81608
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 BMI1  
Affinity Capture-MS Homo sapiens
76 P4HA3 283208
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 MRPS17 51373
Affinity Capture-MS Homo sapiens
78 RBBP5 5929
Co-fractionation Homo sapiens
79 PAPOLA 10914
Co-fractionation Homo sapiens
80 RBMS2 5939
Affinity Capture-MS Homo sapiens
81 MALL  
Affinity Capture-MS Homo sapiens
82 Dtl  
Affinity Capture-MS Mus musculus
83 MAPKAPK2 9261
Affinity Capture-MS Homo sapiens
84 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
85 CEP170P1  
Affinity Capture-MS Homo sapiens
86 CPSF6 11052
Affinity Capture-MS Homo sapiens
87 TP53 7157
Two-hybrid Homo sapiens
88 WWOX 51741
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
89 SAA1 6288
Affinity Capture-MS Homo sapiens
90 RPA1 6117
Affinity Capture-MS Homo sapiens
91 SAP18 10284
Affinity Capture-MS Homo sapiens
92 AIF1  
Affinity Capture-MS Homo sapiens
93 SNRPA 6626
Affinity Capture-MS Homo sapiens
94 PRMT1 3276
Two-hybrid Homo sapiens
95 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
96 CDK6 1021
Affinity Capture-MS Homo sapiens
97 NUP35 129401
Proximity Label-MS Homo sapiens
98 RBM48  
Two-hybrid Homo sapiens
99 DHX40  
Proximity Label-MS Homo sapiens
100 AHCYL2 23382
Affinity Capture-MS Homo sapiens
101 CDK4 1019
Biochemical Activity Homo sapiens
102 HNRNPLL 92906
Affinity Capture-MS Homo sapiens
103 SRSF7 6432
Affinity Capture-MS Homo sapiens
104 SNW1 22938
Affinity Capture-MS Homo sapiens
105 AR 367
Proximity Label-MS Homo sapiens
106 MEN1 4221
Affinity Capture-MS Homo sapiens
107 SP7  
Proximity Label-MS Homo sapiens
108 CPSF3 51692
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
109 CDC5L 988
Affinity Capture-MS Homo sapiens
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