Experiment description of studies that identified CPA4 in sEVs

1 Experiment ID 497 MISEV standards ✘ Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

2 Experiment ID 498 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

Protein-protein interactions for CPA4

Protein Interactor ExoCarta ID Identification method PubMed Species 1 PRSS16 10279 Affinity Capture-MS Homo sapiens 2 ENDOG   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 3 ERCC3   Affinity Capture-MS Homo sapiens 4 IFNA4   Affinity Capture-MS Homo sapiens 5 RHBDD1 84236 Affinity Capture-MS Homo sapiens 6 KLHL11   Affinity Capture-MS Homo sapiens 7 PPP2R2B 5521 Affinity Capture-MS Homo sapiens 8 SLC25A6 293 Affinity Capture-MS Homo sapiens 9 SEP15 9403 Affinity Capture-MS Homo sapiens 10 NDUFS5 4725 Affinity Capture-MS Homo sapiens 11 PIGT 51604 Affinity Capture-MS Homo sapiens 12 CCNC   Affinity Capture-MS Homo sapiens 13 CD22 933 Affinity Capture-MS Homo sapiens 14 LXN 56925 Co-crystal Structure Homo sapiens 15 SLCO6A1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 16 NHLRC2 374354 Affinity Capture-MS Homo sapiens 17 ASCC1 51008 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 18 TCTN3   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 19 TBC1D22B   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 20 CPA2   Affinity Capture-MS Homo sapiens 21 CDK15   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 22 PRG2   Affinity Capture-MS Homo sapiens 23 PCP4   Affinity Capture-MS Homo sapiens 24 ZSCAN12   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 25 RAB9B 51209 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 26 NPPA   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 27 C1orf35   Affinity Capture-MS Homo sapiens 28 OVOL2   Affinity Capture-MS Homo sapiens 29 EBF2   Affinity Capture-MS Homo sapiens 30 ZIC1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 31 PTDSS1 9791 Affinity Capture-MS Homo sapiens 32 GSKIP   Affinity Capture-MS Homo sapiens 33 PLK2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 34 FTH1 2495 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 35 PDPK1 5170 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 36 MYCBPAP 84073 Affinity Capture-MS Homo sapiens 37 ST6GALNAC6 30815 Affinity Capture-MS Homo sapiens 38 ADH1C 126 Affinity Capture-MS Homo sapiens 39 APTX   Affinity Capture-MS Homo sapiens 40 C21orf59 56683 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 41 MOGAT3   Affinity Capture-MS Homo sapiens 42 SH2D3A   Affinity Capture-MS Homo sapiens 43 RPS19BP1   Affinity Capture-MS Homo sapiens 44 MBNL1 4154 Affinity Capture-MS Homo sapiens 45 UBAC2 337867 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 46 KIR3DS1   Affinity Capture-MS Homo sapiens 47 CCDC51 79714 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 48 SMAD9   Affinity Capture-MS Homo sapiens 49 TEFM   Affinity Capture-MS Homo sapiens 50 GALNT9   Affinity Capture-MS Homo sapiens 51 AIRE   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 52 TSPAN6 7105 Affinity Capture-MS Homo sapiens 53 GPR18   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 54 GOT1 2805 Affinity Capture-MS Homo sapiens 55 SARAF   Affinity Capture-MS Homo sapiens 56 E2F6 1876 Affinity Capture-MS Homo sapiens 57 POLR2L 5441 Affinity Capture-MS Homo sapiens 58 ST7   Affinity Capture-MS Homo sapiens 59 OR2A4 79541 Affinity Capture-MS Homo sapiens 60 BEX5   Affinity Capture-MS Homo sapiens 61 CTNNA1 1495 Co-fractionation Homo sapiens 62 GMCL1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 63 GABPA 2551 Affinity Capture-MS Homo sapiens 64 CCR1   Affinity Capture-MS Homo sapiens 65 ATP6AP2 10159 Affinity Capture-MS Homo sapiens 66 STX17 55014 Affinity Capture-MS Homo sapiens 67 B3GALNT1 8706 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 68 RIPPLY3   Affinity Capture-MS Homo sapiens 69 CYLD   Affinity Capture-MS Homo sapiens 70 POC5   Affinity Capture-MS Homo sapiens 71 PHF8   Affinity Capture-MS Homo sapiens 72 HTRA2 27429 Co-fractionation Homo sapiens View the network

Pathways in which CPA4 is involved

No pathways found